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2005 Anatomic Pathology ExamMicroscopic Slide Review
Presented at the57th Annual Meeting of the ACVP
December 3, 2006Tucson, AZ
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
1. Nov/Dec- initial committee meeting @ annual ACVP
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
1. Nov/Dec- initial committee meeting @ annual ACVP
2. Mid-Apr- contributions to section leader
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
1. Nov/Dec- initial committee meeting @ annual ACVP
2. Mid-Apr- contributions to section leader
3. Section leader prepares draft exam-matrix: process/tissue/species
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
4. May/Jun- 2nd committee meeting,
review of draft exam, keys prepared
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
4. May/Jun- 2nd committee meeting,
review of draft exam, keys prepared
5. Mid-Aug- slides, exam questions completed
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
4. May/Jun- 2nd committee meeting,
review of draft exam
5. Mid-Aug- slides, exam questions completed
6. Sep- exam time
2005 Microscopic Slide Exam Reviewpreparing the microscopic exam
1. rat, brain, astrocytoma
2. horse, artery, strongylosis
3. dog, skin, ceruminous gland carcinoma
& dirofilariasis
4. dove, liver, amyloidosis
5. sea lion, kidney, leptospirosis
2005 Microscopic Slide Exam Review
6. ox, lung, atypical interstitial pneumonia
7. cat, intestine, panleukopenia & FIP
8. marmoset, bone, fibrous osteodystrophy
9. dog, salivary gland, infarct
10.ox, spinal cord, fibrocartilaginous embolus
2005 Microscopic Slide Exam Review
11. cat, kidney, lily toxicosis
12. dog, eye, phthisis bulbi
13. rhesus, lung, SIV & CMV
14. walleye, skin, fibrosarcoma
15. ox, heart, ionophore toxicosis
2005 Microscopic Slide Exam Review
16. dog, bone, metastatic carcinoma
17. horse, placenta, aspergillosis
18. dog, liver, autoimmune hemolytic anemia
19. rat (panel), thoracic mass, hibernoma
20. ox (TEM), oral mucosa, MCF
2005 Microscopic Slide Exam Review
Case 7: Tissue from a cat.
• Histopathologic description (12 points)
• Morphologic diagnosis(es) (4 points)
• Cause(s) (4 points)
Case 7: Tissue from a cat.
• Histopathologic description (12 points)– Intestine
• Mucosa– Blunting of villi– Collapse of lamina propria– Dilated crypts with necrotic debris
• Muscularis, serosa, mesentery– Perivascular accumulations of PMNs, macrophages
and fibrin
– O/C• Logical and clear presentation of features
Case 7: Tissue from a cat.
• Morphologic diagnosis(es) (4 points)– Necrotizing enteritis– Pyogranulomatous perivasculitis/peritonitis
• Cause(s) (4 points)– Feline parvovirus (Feline panleukopenia virus)– Feline infectious peritonitis virus
Case 9: Tissue from a dog.
• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)
Case 9: Tissue from a dog.
• Histopathologic description (14 points)– Salivary gland (ID)
• Vasculature– Thrombosis
• Acini– Coagulative necrosis– Inflammation– Granulation tissue
Case 9: Tissue from a dog.
• Histopathologic description (14 points)– Salivary gland (ID)
• Septa– Inflammation– Hemorrhage– Fibrosis
• Ducts– Inflammation– Necrosis– Squamous metaplasia
– O/C• No points allotted
Case 9: Tissue from a dog.
• Morphologic diagnosis(es) (6 points)
– Necrotizing sialoadenitis with thrombosis (infarction) and squamous metaplasia
Case 11: Tissue from a cat.
• Histopathologic description (13 points)
• Morphologic diagnosis(es) (4 points)
• Most likely cause (3 points)
Case 11: Tissue from a cat.
• Histopathologic description (13 points)– Distribution
– Tubular epithelium• Degeneration • Necrosis• Regeneration
Case 11: Tissue from a cat.
• Histopathologic description (13 points)– Casts
• Granular• Protein
– Mineralization
– O/C • Logical and clear presentation of features
Case 11: Tissue from a cat.
• Morphologic diagnosis(es) (4 points)– Diffuse tubular necrosis with casts
• Most likely cause (3 points)– Lily toxicosis– Aminoglycoside antibiotic toxicosis
Case 16: Tissue from a dog.
• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)
Case 16: Tissue from a dog.
• Histopathologic description (14 points)– Bone
• Neoplasm: location, pattern, cell morphology, cell borders, cytoplasm, nuclear morphology, nucleoli, mitoses, anisokaryosis, necrosis
• Proliferation of new bone • Resorption of trabecular bone
Case 16: Tissue from a dog.
• Histopathologic description (14 points)– Muscle degeneration and atrophy
– O/C• Logical and clear presentation of features• Primary vs. secondary processes
Case 16: Tissue from a dog.
• Morphologic diagnosis(es) (6 points)– Metastatic transitional cell carcinoma in bone– Hyperostosis– Muscular atrophy and degeneration
Case 19: Tissue from a rat.
• Microscopic description (16 points)
• Morphologic diagnosis (4 points)
Case 19 (panel): Tissue from a rat.
• Microscopic description (16 points)– A. H&E
• Description: pattern, cell morphology, cell borders, cytoplasmic vacuoles (fat), nuclear morphology, multinucleated cells, nucleoli, no mitotic figures
– B. Oil-Red-O• Positive cytoplasmic staining = lipid
Case 19 (panel): Tissue from a rat.
• Microscopic description (16 points)
– C. Mitochondrial uncoupling protein-1 immunohistochemistry
• Reactivity indicates presence of numerous mitochondria
Case 19 (panel): Tissue from a rat.
• Microscopic description (16 points)– D. TEM
• Many mitochondria• Some mitochondrial swelling• Fat droplets• Morphology consistent with brown fat
Case 19 (panel): Tissue from a rat.
• Morphologic diagnosis (4 points)– Hibernoma
2005 Microscopic Slide Exam Reviewscoring
Proportion (%) Passed Mean Score Range of Scores
2003 2004 2005 2006 2003 2004 2005 2006 2003 2004 2005 2006
45 72 47 61 57 63 57 62 71-37 82-11 75-31 83-7
