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Sperm Analysis

human

CASA SystemsISAS v1INTEGRATED SPERM ANALYSIS SYSTEM

ISASv1 has been designed using the most advanced image

analysis technology. ISASv1 applications include veterinarian cl inic,

scientific research, l ivestock production and pharmaceutical and

reproductive toxicology. ISASv1 is the most complete and easiest-to-

use system which, furthermore, works in different hardware and

operating-system conditions, in order to adapt as much as possible to

the needs of our users.

ISASv1 is a multi-specie system (stal l ion, bul l , boar, goat, rabbit,

dog, rat, mice, rooster, ram, cod, shark, tuna, anguil la, . . . ). The

supported species is continuously growing.

ISASv1 works with cameras up to 200 frames per second,

improving track analysis. ISASv1 has been special ly designed for

quality control programs, al lowing the analysis of different formats of

video fi les (Windows AVI , Windows Media Video, MPEG-4, DVD, and

many others) and images (jpg,png,bmp,gif, tif, . . . ).

ISASv1 is completely modular, so each module might work both

independently and jointly.

Accurate

Fast

Flexible

User friendly

Complete

CASA Systems

Morphometry

Moti l ity

ISAS v1 Motility performs the sperm moti l ity and

concentration analysis. I t works in a very great range

of species and each species has its own

configuration and analysis algorithms.

Depending on the sample, both negative or positive

phase contrast can be used. The moti l ity analysis of

one field is done in real-time (less than 1 second),

and it can be configured to analyze moti l ity up to 250

frames, at 200 frames per second and with

concentrations higher than 1 000 sperms/field.

Aditional ly to concentration , motility and

progressivity percentages, fol lowing kinetic

parameters are calculated: VCL, VAP, VSL, LIN ,

STR, WOB, ALH , BCF, DNC, MAD, . . .

ISASv1 Morphometry performs the morphologic

and morphometric spermatozoa analysis from

stained samples or fresh samples (using phase

contrast optics). The morphometric analysis is done

using different optics depending on species (40x, 60x

or 1 00x) in short time.

ISASv1 Morphometry gives 1 4 morphometric

parameters: head size (area, length, width and

perimeter), head shape (el l ipticity, elongation,

roughness, regularity), acrosome percentage, head

color level, midpiece size (area and width) and

midpiece insertion (angle and distance).

DNA Fragmentation

Vital ity

CASA Systems

ISASv1 DNA Fragmentation automatical ly performs

the analysis of the percentage of DNA fragmentated

sperm percentage.

The fragmentation analysis of one field is done using

20x or 40x objectives in real-time (less than 1

second). Depending on the species, information

about size of core and halo is used to define the DNA

fragmentation level.

ISASv1 DNA Fragmentation requires the use of

different kits, l ike Halotech DNA, based on the Sperm

Chromatin Dispersion test (SCD) technology, or

Tunnel Assay Test.

ISAS v1 Vitality performs automatic viabil ity analysis

of sperm cells. We need to determine the real

percentage of viabil ity cel ls in a sample, and to

perform accurately this analysis we need to use

fluorescent techniques with their correspondent use

of appropriate staining kits. Anyway, the classic

eosine-nigrosine stain can be also used now!

USIA OVOOocyte measurement system

ANALYSISCHARACTERISTICS● Unlimited number of fields and cells

to be analyzed

● Capture from camera or from

different image file formats (JPEG,

TIFF, PICT, GIFF)

● Specially designed for Quality

Control Programs

● Capability to desing specific

measurements

● Interactive analysis of predefined

variables

DATA AND REPORTCHARACTERISTICS● Measure distances, angles, areas,

perimeters F.

● You can define your personalized

output data file

● Customizable reports with tables,

graphics and cell images

● Database allows to perform different

sortings and historical revisions

● Standard interconexion

OVO SYSTEM

ISAS LABThe best solution for the fastestand reliable clinic semen diagnosis

ISAS-Lab ® allows seminal reviews in theordinary circumstances of a laboratory foranalysis, both for the determination ofsemen quality, as the monitoring of

samples during processing in assistedreproductive techniques. The system

assesses various mobility characteristics,sperm concentration and morphology for acorrect determination of sperm count, rapidand reliable, following the WHO criteria.

To obtain the mostcomplete report just in

one click

CASA Systems

MOTILITY,CONCENTRATIONAND BASIC MORPHOLOGYat 20x under negative phase contrast

MOTILITY ANDCONCENTRATIONat 1 0x under negative phase contrast

COMPLETEMORPHOLOGYat bright field 40x, following WHO and

Strict criteria

Is the perfect tool for routine clinic analysis!

CASA Systems

SPERMTRACKThe new counting chamber

The II SSAASS product family for

seminal analysis grows with a new

tool that improves substantial ly

preexisting counting chambers.

Following the semen droplet

displacement procedure,

SSPPEERRMMTTRRAACCKK, el iminates most

disadvantages presented by less

efficient products found in the

market. SSPPEERRMMTTRRAACCKK design

facil itates its use in the microscope

and also during cleaning,

manipulation and storing.

1 0/20 microns

SSPPEERRMMTTRRAACCKK 1100 achamber of 10 µm depthfor human traditionalclinical analysis.

SSPPEERRMMTTRRAACCKK 2200 with 20µm follows the latest

recommendations ofWWHHOO

llaabboorraattoorryy mmaannuuaall 22001100 ,and it also extends its usefor veterinary purposes,due to the more naturalfreedom ofsperm cell

Traceability

Independent serie numbers on thecover and on the chamber, allow afull traceability under IISSOO1133448855

standards.Reliability and security.

SSPPEERRMMTTRRAACCKK is lessthick than other similarproducts. the condenserlight from the microscopearrives to the samplemuch more focused,improving the optical

quality and preventing thegeneration of commonvisual artifacts in other

devices.

Optical quality

ACCESSORIES

Accessories

ISASD4CDisposable counting chamber

• Accurate heights: 1 2µm, 1 6µm and 20µm

• 4 chambers per slide

• Non-toxic

• Improved design:

Better fill ing and reading

• Cost-effective

• Safe and secure

Package: 25 slides/1 00 analysis box.

HEATED PLATES• Adjustable temperature & calibration.

• 24/40 holes for dilution tubes

• Warming area for counting chambers

• Fast start-up: from 22ºC to 37ºC in 5 minutes

• Sensing resolution: 0.03ºC

• Stability: 0.1 ºC

• Temperature absolute error: 0.5ºC

ISAS HEATSYSTEM• Heating system for any microscope stage.

• Adjustable temperature & calibration.

• Fast start-up: from 22ºC 37ºC in 45 sec.

• Sensing resolution: 0.03ºC

• Stability: less than 0.1 ºC

• Temperature absolute error: 0.5ºC

Any kind of morphometric measurements (for morphology, vital ity, DNAFragmentation analysis) needs the staining of the cells. Different kits can be used for

each purpose, and anyone must to look for that with better adjustment to hisrequirements. Our systems, can work with different kits but for human we

recommend the use of rapid Papanicolau protocol , fol lowing WHOrecommendations. But also other techniques l ike Diff-quick or Eosine Nigrosine are

availables.We have developed another useful kits.

STAIN KITS

DIFF QUICKThis is an alternative toPapanicolau techniqueused for most people,

but not thatrecommended by WHO.I t includes the fixative

and two stainingsolutions in bottles of

500 ml

FLUORESCENTDIESWe have preparedsolutions of acridineorange, propidium

iodide, hoetsch; usefulfor the determination of

l ive/dead cells,mitochondrial activity,and other probes.

QC-BEADSThis kit includes two

bottles of 4 mlcontaining solutions(high and low) of

defined concentration oflatex spheres. I t is usedfor the calibration of

concentrationcalculations both inmanual and CASA

analysis.

EOSINENIGROSINEFollowing the WHOprotocol we have

performed an Eosine-Nigrosine kit for the

determination of headmembrane alterations

evaluation. I t ispresented in bottles of

1 25 and 250 ml.

HOSTFollowing the WHOprotocol we haveperformed an Hostsolution kit for thedetermination of tai lmembrane alterations

evaluation. I t ispresented in bottles of

1 25 and 250 ml.

SHORTPAPANICOLAUFollowing the WHOrecommendation we

have developed a shortPapanicolau kit. The kitincludes not only thestain solutions, but

fixative and mountingmedium. I t is able for

250 smears.

ACCESSORIES

FLECCO is a flexible counter for any kind of purposes. Forgot the "digital" cellcounters. With Flecco you can define as much keys as you need and the softwaresave for you the data directly in excel format.

Moreover, you can have reference images into the program to use them fortraining of technicians.

Designed forundertaking

manual countingsuch as the

leukocyte formula,spermatozoondiagrams or

bacterial colonycounting.

It allows the user introducing anyother counting in a flexiblemanner.

It includes a module which allowstime assignment for both eventsand total analysis.

Besides, it might includereference-image galleries.

FLECCOFlexible counter system

COUNTER SYSTEM

Projectes i Serveis R+D S.L.Catedratico Agustin Escardino, 9Edificio 3 (CUE), Planta 146980 PaternaSpain+34 961196060 (phone)+34 961318466 (fax)proiser@proiser.com (email)http://www.proiser.com

DISTRIBUTOR:

April 2011