(1)Start lasers, then find sample using VIS

(2)Set configuration using LSM

(3)Set scan resolution, speed & repetition in Mode

Initial pixal number, scan speed, averaging, zoom and pinhole size are set in stored configurations. These should be optimised after preliminary images.

(4)Set pinhole, excitation & detection for each Channel

Single track

Multi trackUse Find to get initial image, Split and Palette to adust

(5)Control Zoom and Crop in Mode and Select

(6)Set Z stack with first/last & optical slice

(7)Display 3D using projection

(8)Open database for image retrieval

(9)Time Series and Edit Bleaching

(10)Using Lambda stacks, Save to Spectra DB for Linier Unmixing