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APPLICATION FORMYSP-Travel Fellowships for 13th FAOBMB Congress, Bangkok, November 23-29, 2012.Name of Applicant: Ms/Mr/Dr Mehdi Sahihi Position: Ph.D. Student / Postdoctoral FellowAddress of Applicant: Chemistry department, Isfahan University of Technology, Isfahan 84156/83111, IranEmail address: [email protected] Membership of FAOBMB Constituent Society: Iranian Biochemistry Society Date of Birth: 30/08/1981 Applicants must be no more than 35 years of age at the closing date for appli
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APPLICATION FORM
YSP-Travel Fellowships for 13th FAOBMB Congress, Bangkok,
November 23-29, 2012.
Name of Applicant: Ms/Mr/Dr Mehdi Sahihi
Position: Ph.D. Student / Postdoctoral Fellow
Address of Applicant: Chemistry department, Isfahan University of Technology, Isfahan 84156/83111, Iran
Email address: [email protected]
Membership of FAOBMB Constituent Society: Iranian Biochemistry Society
Date of Birth: 30/08/1981
Applicants must be no more than 35 years of age at the closing date for application:
(31 July, 2012).
Proof of Age: (Certification by the Head of your Institution or Department is required)
I certify on the basis of official documentation which I have seen (e.g. passport, birth
certificate or driver’s licence) that the date of birth of the applicant Mr Mehdi Sahihi
Is 30/08/1981, as shown above.
Signed: Y. Ghayeb
Position: Head of chemistry department
Have you previously received an FAOBMB Travel Fellowship? If yes, please indicate
when.
No
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Curriculum Vitae
Provide a brief CV showing education and research training, including any prizes or
awards:
EDUCATION and RESARCH
2007-2012 Ph.D. Student in Biophysical Chemistry, Isfahan University of Technology,
Isfahan, Iran.
Thesis: Thermodynamic stability and Binding properties of β-lactoglobulin
Supervisors: Prof. Abdol-khalegh Bordbar, Dr. Yousef Ghayeb.
Courses: Quantum Chemistry, Quantum Mechanics of Many Particle systems,
Laser Spectroscopy, Angular Momentum, Statistical Thermodynamics.
2004-2006 M. Sc. in Physical Chemistry, Isfahan University of Technology, Isfahan, Iran.
Thesis: Solubility of Fumaric Acid in SC-CO2
Supervisor: Prof. Hassan Ghaziaskar.
Courses: Advanced Physical Chemistry, Thermodynamics, Chemical Kinetics,
Quantum Chemistry, Statistical Thermodynamics.
1999-2003 B. Sc. in Pure Chemistry, the University of Isfahan, Isfahan, Iran.
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List of Publications in International Journals*
1- M. Sahihi, A.K. Bordbar, Y. Ghayeb. "Solubility of maleic acid in supercritical carbon
dioxide", J. chem. eng. Data. 55, 2596-2599 (2010) (IF 1.693)
2- M. Sahihi, A.K. Bordbar, Y. Ghayeb. "Thermodynamic denaturation of β-lactoglobulin in
the presence of cetylpyridinium chloride", The Journal of chemical thermodynamics. 42,
1423-1428 (2010) (IF 2.422)
3- M. Sahihi, A.K. Bordbar, Y. Ghayeb. "Thermodynamic stability and retinol binding
property of β-lactoglobulin in the presence of cationic surfactants", The Journal of chemical
thermodynamics. 43, 1185-1191 (2011) (IF 2.422)
4- M. Sahihi, A.K. Bordbar, Y. Ghayeb, N. Fani. "Structure-Function Relationship of β-
Lactoglobulin in the Presence of Sodium Dodecylbenzenesulfonate", The Journal of chemical
thermodynamics. 52, 16-23 (2012) (IF 2.422)
5- "Fluorescence Spectroscopic Study on Interaction of Retinol with β-lactoglobulin in the
Presence of Cetylpyridinium Chloride", Spectroscopy, 27, 27-34 (2012) (IF 0.932)
6- "The Interaction of Polyphenol Flavonoids with β-lactoglobulin: Molecular Docking and
Molecular Dynamics Simulation Studies", Journal of Macromolecular Science Part B, In
Press (IF 0.739)
7- M. Sahihi, F. Mohammadi, A.K. Bordbar, Y. Ghayeb, A.A. Saboury. "Interaction of
Bisdemethoxycurcumin and Diacetylbisdemethoxycurcumin with β-lactoglobulin:
Spectroscopic and Molecular Dynamics Simulation Studies", Journal of Structural Biology,
Under Review (IF 3.406)
8- "Interaction of β-Lactoglobulin with Resveratrol: Molecular Docking and Molecular
Dynamics Simulation Studies", Protein Journal, Under Review (IF 1.038)
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Abstract
Interaction of Bisdemethoxycurcumin and Diacetylbisdemethoxycurcumin with β-
lactoglobulin: Spectroscopic and Molecular Dynamics Simulation Studies
M. Sahihi
a,*, F. Mohammadi
b,c, A.Khalegh Bordbar
b,*, Y. Ghayeb
a, A.Akbar Saboury
d a Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran b Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran c Department of Chemistry, Institute for Advanced Studies in Basic Sciences (IASBS), Gava Zang, Zanjan 45137-66731, Iran d Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
This study demonstrates the interactions of bisdemethoxycurcumin (BDMC) and
diacetylbisdemethoxycurcumin (DABC) as the bioactive constituents of turmeric with bovine
β-lactoglobulin (BLG) variant B using fluorescence, circular dichroism (CD), molecular
docking, and molecular dynamics simulation methods. The binding constant for formation of
BLG-BDMC and BLGDABC complexes were 8.99 ± 0.1 × 104 M
-1 and 1.87 ± 0.1 × 10
2 M
-1,
respectively. The distance between BLG and BDMC was obtained based on Förster`s theory
of non-radiative energy transfer. The secondary structure contents of BLG in the presence of
ligands were obtained by analysis of CD spectra.
Molecular docking studies revealed that BDMC and DABC bind to the surface of the protein
by four and one hydrogen bond interactions, respectively. Finally, molecular dynamics
simulation results show that the binding of BDMC to BLG causes the conformational change
of BLG and the structure of binding site remains rigid during the simulation of two
complexes.
KEYWORDS: Bisdemethoxycurcumin; Diacetylbisdemethoxycurcumin; Bovine β-
lactoglobulin; Fluorescence quenching; Circular dichroism, Molecular dynamics simulation.
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Personal Statement
My interest lies in the field of biophysical chemistry with particular attention to the structure-
function relationship of proteins, molecular dynamics simulation of proteins and other
biomacromolecules, natural ligands and drug design.
At this point in time I am seeking to present my experience and training in protein
science and MD simulation to the contributors of FAOBMB Congress.
Given the opportunity, I am confident that I will be able to transfer my experiences to
other contributors while at the same time developing my knowledge of biochemistry
and molecular biology that I will need to succeed in my future research endeavors.
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To whom it may concern
Mr. Mehdi Sahihi asked me to write a recommendation letter regards to his recent application
for YSP-Travel Fellowships for 13th FAOBMB Congress, Bangkok. Actually it is my great
pleasure to do it. I know Mehdi since 2000 when he was BSc student in department of
chemistry, University of Isfahan. He passed some of his physical chemistry courses with me
and was one of the best students in the class. After successful graduation in BSc course, he
immediately admitted as MSc student of Physical Chemistry in Isfahan University of
Technology, and graduated well in this course in 2007. He immediately admitted as PhD
student of Physical Chemistry in Isfahan University of Technology as one of the high rank
universities in Iran with respect to his excellent records. My familiarity was become perfect
when he did his PhD thesis under the supervision of me and Dr. Ghayeb. His PhD thesis was
in the area of Biophysical Chemistry. He did a very comprehensive study on structure-
function relationship of beta-lactoglobulin in the presence various drugs and ligands. He has
published several valuable papers in peer review ISI journals.
With respect to the above mentioned points I strongly recommend him for this fellowship.
Please do not hesitate to contact me if you need any other information.
With best regards
A. Khalegh Bordbar
Professor of Biophysical Chemistry
University of Isfahan
Isfahan, 81746-73441
Iran
Tel.: +98-3117932710
Fax: +98-3116689732
Emails: [email protected] and [email protected]
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To whom it may concern
It is my pleasure to recommend Mr. Mehdi Sahihi for the YSP-Travel Fellowships for
13th FAOBMB Congress, Bangkok. Mehdi is PhD student in my lab and is one of the
most outstanding researchers to emerge from my lab. I recommend him to you highly.
In my lab Mr. Mehdi Sahihi works on thermodynamic stability and binding properties
of beta-lactoglobulin (BLG). He studies the interaction of Ionic surfactants and
natural ligands with BLG by spectroscopic and molecular dynamics simulation
methods. Mr. Sahihi published 5 papers from his studies which attest to his hard
work, biophysical insight and outstanding writing skills. Also some of his works by
MD method are not yet published. Mr. Sahihi proved himself an outstanding
researcher. Mr. Sahihi is also a terrific citizen and a leader in my lab. In short I give
Mehdi my highest recommendation. He is one of my finest students, an outstanding
researcher, and I expect him to be one of top researchers in his field. I strongly
recommend him and will appreciate your assistance to his application.
Yours Sincerely,
Dr. Yousef Ghayeb
Assos. Professor in Department of Chemistry
Isfahan University of Technology, Isfahan- 84156/83111, Iran
Tel.: +98-311-3913252
Fax: +98-311-3912350
E-mail: [email protected]
