Mucins are a family of heavily glycosylated proteins, and are the main components of mucosa such as saliva, tear, gastric fluid or enteric fluid. Basic configuration of mucin are macromolecules linked ramiform sugar chain to peptide framework. The heterogeneous property of sugar chain makes them diverse; the molecule has various functions, such as specific molecular recognition. Some of the sugar chains recognize a specific protein derived from virus and bacteria. Mucins play a role as mucosal barrier function in the gut, prevent the translocation of pathogens and toxins into blood vessel beyond the intestinal wall. We can provide this unique kit especially for functional food development and infection research.

Mucin and IgA prevent invasion of bacteria and toxinsMucin and IgA protect the translocation of pathogen and toxin into blood vessel beyond the intestinal wall, as an intestinal barrier function

Intestinal barrier function.

Mucin Assay Kit For research on health and beauty

Structure of Mucin

GalNAcSer

Thror ･･･

Description Cat. No. Quantity Storage

Mucin Assay Kit CSR-FFA-MU-K01E 1 Kit 4℃

Kit Component● Buffer solution A (tablet) x3, 100 mL ● Buffer solution B x1, 25 mL ● Buffer solution C x1, 25 mL ● Reagent A x1, 1.0 mL

Ordering Information

● Reagent B x2, 1.5 mL ● Standard solution (N-acetylgalactosamine, 250 µg / mL) x1, 1.0 mL ● Enzyme solution x1, 1.5 mL

Assay and Detection

Measure three days worth of feces

ResultsThe berry polyphenol addition groups enhanced the amount of mucin as compared to High-Fat diet groups.

Prepare 5 experimental groups (6 rats per group)① Control ⇒ 4 weeks　　②③④⑤ High-Fat diet ⇒ 4 weeks

5 experimental groups (6 rats per group)　① Control ⇒ 4 weeks　② High-Fat diet ⇒ 4 weeks　③ High-Fat diet + Aronia ⇒ 4 weeks　④ High-Fat diet + Haskap ⇒ 4 weeks　⑤ High-Fat diet + Bilberry ⇒ 4 weeks

Mucin Assay Kit For research on health and beauty

Experimental Example Effects of berry polyphenols administration on the intestinal barrier function in high-fat diet fed rats.

Standard Curve

N-acetylglucosomine (µg/mL)

Fluorescence (336/383 nm)

y=-0.3534x2+199.06x+373.25R2=0.9992

30000

25000

20000

15000

10000

5000

00 100 200 300

Mucin(equivalent(GalNAc)mg/g of feces)

3.5

3

2.5

2

1.5

1

0

0.5

Fecal mucin

Measurement Example

Control High FatAroniaHigh Fat+ High Fat+ High Fat+

Haskap Bilberry

Dietary polyphenols derived from aronia, hascup and bilberry, markedly-elevated the amount of fecal mucin. (n=5)

References● Susumu Honda, et al,: Fluorimetric Determination of Reducing Carbohydrates with2-Cyanoacetamide and Application to Automated Analysis of Carbohydrates as Borate Complexes. (1980) Analytical Chemistry, Vol. 52, No. 7

● Bovee-Oudenhoven IM, et al.: Increasing the intestinal resistance of rats to the invasive pathogen Salmonella enteritidis: additive effects of dietary lactulose and calcium. Gut 40: 497-504, 1997.

● Crowther RS, et al,: Fluorometric assay of O-linked glycoproteins by reaction with 2-cyanoacetamide. Anal Biochem 163: 170-174, 1987.● Okazaki Y, et al,: Consumption of curcumin elevates fecal immunoglobulin A, an index of intestinal immune function, in rats fed a high-fat diet. J Nutr Sci Vitaminol (2010); 56(1):68-71.

● Yukako Okazaki, et al,: Consumption of a Resistant Protein, Sericin, Elevates Fecal Immunoglobulin A, Mucins, and Cecal Organic Acids in Rats Fed a High-Fat Diet. (2011) The Journal of Nutrition, 21, 10.3945/jn.111.144246.

● Zaki Utama, et al,: Tempe Consumption Modulates Fecal Secondary Bile Acids, Mucins, Immunoglobulin A, Enzyme Activities, and Cecal Microflora and Organic Acids in Rats. Foods Hum Nutr (2013) 68:177-183

● Taira R., Yamaguchi S., Shimizu K., Nakamura K., Ayabe T., and Taira T. Bacterial cell wall components regulate adipokine secretion from visceral adipocytes. Journal of Clinical Biochemistry and Nutrition Vol. 56 (2015) No. 2 p. 149-154

● Taira T., Yamaguchi S., Takahashi A., Okazaki Y., Yamaguchi A., Sakaguchi H. and Chiji H. Dietary polyphenols increase fecal mucin and immunoglobulin A and ameliorate the disturbance in gut microbiota caused by a high fat diet. J Clin Biochem Nutr. 2015 Nov; 57(3): 212-6.

Assay and Detection

For research use only. Not for diagnostic use.　　　 www.cosmobio.com

10165