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Cellular Cellular ReproductiReproducti

onon

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Types of Cell Types of Cell ReproductionReproduction

Asexual reproductionAsexual reproduction involves a involves a single cellsingle cell dividing to make dividing to make 2 2 new, identical daughter cellsnew, identical daughter cells

3 Reasons why cells undergo 3 Reasons why cells undergo asexual Reproduction:asexual Reproduction:

1.1. GrowthGrowth2.2. RepairRepair3.3. ReplacementReplacement

Examples: somatic cells (body), cancer Examples: somatic cells (body), cancer cellscells

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Five Phases of the Cell Five Phases of the Cell CycleCycle

GG11 - primary growth phase - primary growth phaseS – synthesis; DNA replicatedS – synthesis; DNA replicatedGG22 - secondary growth phase - secondary growth phase

**collectively these 3 stages **collectively these 3 stages are called interphaseare called interphase

M - mitosisM - mitosisC - cytokinesisC - cytokinesis

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The Cell CycleThe Cell Cycle

DaughteDaughter Cellsr Cells

DNA DNA CopiedCopied

Cells Cells MaturMatur

ee

Cells prepare Cells prepare for Divisionfor Division

Cell Divides into Cell Divides into Identical cellsIdentical cells

http://www.cellsalive.com/mitosis.htm

Interphase

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• Occurs before Mitosis begins• Chromosomes are Chromosomes are copiedcopied (# doubles) (# doubles)• Chromosomes appear as threadlike coils Chromosomes appear as threadlike coils

((chromatinchromatin) at the start, but each ) at the start, but each chromosome and its copy (sister chromosome) chromosome and its copy (sister chromosome) change to change to sister chromatidssister chromatids at end of this at end of this phasephase

Remember:Remember:GG11 - primary growth phase - primary growth phaseS – synthesis; DNA replicatedS – synthesis; DNA replicatedGG22 - secondary growth phase - secondary growth phase

**collectively these 3 stages are **collectively these 3 stages are called called interphaseinterphase

Photographs from: http://www.bioweb.uncc.edu/biol1110/Stages.htmPhotographs from: http://www.bioweb.uncc.edu/biol1110/Stages.htm

Animal CellAnimal Cell Plant CellPlant Cell

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MitosisMitosisDivision of the Division of the

nucleusnucleusAlso called Also called

karyokinesiskaryokinesisOnly occurs in Only occurs in

eukaryotes eukaryotes (plants and (plants and animal cells)animal cells)

Has Has fourfour stages stagesDoesn’t occur in Doesn’t occur in

some cells such some cells such as as brain cellsbrain cells

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Four Mitotic StagesFour Mitotic Stages

ProProphasephaseMetaMetaphasephaseAnaAnaphasephaseTeloTelophasephase

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Early ProphaseEarly ProphaseChromatinChromatin in nucleus condenses to in nucleus condenses to

form form visible chromosomesvisible chromosomesMitotic spindle Mitotic spindle forms from fibers in forms from fibers in

cytoskeleton or cytoskeleton or centrioles (animal)centrioles (animal)

ChromosomesChromosomes

NucleolusNucleolus CytoplasmCytoplasm

Nuclear MembraneNuclear Membrane

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Late ProphaseLate ProphaseNuclear membrane & nucleolusNuclear membrane & nucleolus

are broken downare broken downChromosomesChromosomes continue continue

condensing & are condensing & are clearly visibleclearly visibleSpindle fibers called Spindle fibers called

kinetochoreskinetochores attach to the attach to the centromerecentromere of each chromosome of each chromosome

SpindleSpindle finishes forming finishes forming between the between the poles of the cellpoles of the cell

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Late ProphaseLate Prophase

Nucleus & Nucleolus have disintegratedNucleus & Nucleolus have disintegrated

ChromosomeChromosomes s

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Review of ProphaseReview of Prophase

What the cell What the cell looks likelooks like

What’s happeningWhat’s happening

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MetaphaseMetaphaseChromosomes, attached to the Chromosomes, attached to the kinetochore kinetochore

fibersfibers, move to the center of the cell, move to the center of the cellChromosomes are now lined up at the Chromosomes are now lined up at the

equatorequatorMetaphase= Metaphase= “Middle”“Middle”

Pole of Pole of the Cellthe Cell

Equator of CellEquator of Cell

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MetaphaseMetaphase

ChromosoChromosomes lined mes lined at the at the EquatorEquator

The poles The poles of a cellof a cell

Spindle Spindle FibersFibers

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MetaphaseMetaphase

The pole of The pole of the cellthe cell

ChromosomesChromosomes at at EquatorEquator

Animal CellAnimal Cell

Plant CellPlant Cell

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Review of MetaphaseReview of Metaphase

What the cell What the cell looks likelooks like

What’s What’s occurringoccurring

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AnaphaseAnaphaseOccurs Occurs

rapidlyrapidlySister Sister

chromatidschromatids are pulled are pulled apart to apart to opposite opposite polespoles of the of the cell by cell by spindle spindle fibersfibers

Sister Sister Chromatids Chromatids

being being separatedseparated

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Anaphase ReviewAnaphase Review

What What the cell the cell looks looks likelike

What’s What’s occurrioccurri

ngng

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TelophaseTelophaseSister chromatids at Sister chromatids at opposite polesopposite polesSpindle Spindle disassemblesdisassemblesTwo new nuclei formTwo new nuclei formNuclear envelopeNuclear envelope forms around forms around

each set of sister chromatidseach set of sister chromatidsNucleolusNucleolus reappears reappearsCYTOKINESISCYTOKINESIS occurs occursChromosomes reappear as Chromosomes reappear as

chromatinchromatin

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Comparison of Anaphase & Comparison of Anaphase & TelophaseTelophase

Telophase

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Animal Animal CellCell

Plant CellPlant Cell

Nuclei Nuclei reformingreforming

Polar Polar microtubules microtubules

continue continue elongationelongation

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CytokinesisCytokinesisMeans division of the Means division of the

cytoplasmcytoplasmOccurs after mitosisOccurs after mitosisDivisionDivision of cell into two, of cell into two,

identical halves called identical halves called daughter daughter cellscells

In plant cells, cell plate In plant cells, cell plate forms forms at the equator to divide cellat the equator to divide cell

In animal cells, In animal cells, cleavage furrow cleavage furrow forms to split cellforms to split cell

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CytokinesisCytokinesisCleavage Cleavage furrow in furrow in

animal cellanimal cell

Cell plate in Cell plate in plant cellplant cell

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Daughter Cells of MitosisDaughter Cells of MitosisHave the Have the same number of same number of

chromosomes as each other chromosomes as each other and as the parent cell and as the parent cell from from which they were formedwhich they were formed

Identical Identical to each other, but to each other, but smaller than parent cellsmaller than parent cell

Must Must grow in sizegrow in size to become to become mature cells mature cells (G(G11 of Interphase) of Interphase)

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Review Eukaryotic Cell Review Eukaryotic Cell DivisionDivision

Used for Used for growth and growth and repairrepair

Produce two new cells Produce two new cells identical to the original identical to the original cellcell

Cells are Cells are diploid (2n)diploid (2n)Chromosomes Chromosomes

during Metaphase during Metaphase of mitosisof mitosis

ProphaProphasese

MetaphaseMetaphase AnaphaseAnaphaseTelophaseTelophaseCytokinesisCytokinesis

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Mitotic StagesMitotic Stages

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The Cell Cycle AnimationThe Cell Cycle AnimationYou should be able to name each You should be able to name each

stage!!stage!!

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RevieReview of w of

MitosiMitosiss

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InterphaseInterphase

ProphaseProphase

MetaphaseMetaphaseAnaphaseAnaphase

TelophaseTelophase

Name the Mitotic Stages:Name the Mitotic Stages:

Name Name this?this?

Name this?Name this?

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Mitosis in Onion Root Mitosis in Onion Root TipsTips

Do you see any stages of mitosis?Do you see any stages of mitosis?

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Draw & Learn these Draw & Learn these StagesStages

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Draw & Learn these Draw & Learn these StagesStages

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Test Yourself Test Yourself over Mitosisover Mitosis

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Mitosis QuizMitosis Quiz

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Mitosis QuizMitosis Quiz

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Name the Stages of Name the Stages of Mitosis:Mitosis:

Interphase

Early prophase

Mid-ProphaseLate Prophase

Metaphase

Late Anaphase

Early Anaphase

Early Telophase

, Begin

cytokinesis

Late telophase, Advanced cytokinesis

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Identify the StagesIdentify the Stages

Early, Middle, & Late Prophase Early, Middle, & Late Prophase

Late Late ProphaseProphase

MetaphaseMetaphase AnaphaseAnaphase

Late Late AnaphaseAnaphase

TelophaseTelophase Telophase & Telophase & CytokinesisCytokinesis

??

?? ?? ??

?? ?? ??

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Locate the Four Mitotic Locate the Four Mitotic Stages in PlantsStages in Plants

MetaphasMetaphasee

ProphaseProphase

AnaphaAnaphasese

TelophaseTelophase

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Uncontrolled Mitosis If mitosis is not If mitosis is not

controlledcontrolled, , unlimited cell unlimited cell division occurs division occurs causing cancerous causing cancerous tumorstumors

OncogenesOncogenes are are special proteins special proteins that increase the chance increase the chance that a normal cell that a normal cell develops into adevelops into a tumor cell tumor cell

Cancer Cancer cellscells

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MeiosisMeiosisFormation of Formation of

GametesGametes (Eggs & (Eggs & Sperm)Sperm)

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Facts About MeiosisFacts About Meiosis

Preceded by interphase which Preceded by interphase which includesincludes chromosome chromosome replicationreplication

TwoTwo meiotic divisions --- meiotic divisions --- Meiosis I and Meiosis IIMeiosis I and Meiosis II

Called Reduction- divisionCalled Reduction- divisionOriginal cell is diploid (2n)Original cell is diploid (2n)Four daughter cellsFour daughter cells produced produced

that are that are monoploid (1n)monoploid (1n)

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Facts About MeiosisFacts About MeiosisDaughter cellsDaughter cells contain half the contain half the

number of chromosomesnumber of chromosomes as the as the original celloriginal cell

ProducesProduces gametesgametes (eggs & (eggs & spermsperm))

Occurs in the Occurs in the testestestes in males in males ((SpermatogenesisSpermatogenesis))

Occurs in the Occurs in the ovariesovaries in in females (females (OogenesisOogenesis))

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Start with Start with 46 double stranded 46 double stranded chromosomes (2n)chromosomes (2n)After 1 division - After 1 division - 23 double 23 double stranded chromosomes (n)stranded chromosomes (n)After 2nd division - After 2nd division - 23 single 23 single stranded chromosomes (n)stranded chromosomes (n)  Occurs in our Occurs in our germ cellsgerm cells that that produce gametesproduce gametes

More Meiosis FactsMore Meiosis Facts

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Why Do we Need Why Do we Need Meiosis?Meiosis?

It is the fundamental basis It is the fundamental basis of of sexual reproductionsexual reproduction

Two haploid (1n) gametes Two haploid (1n) gametes are brought together are brought together through through fertilizationfertilization to form to form a diploid a diploid (2n) zygote(2n) zygote

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Fertilization – “Putting Fertilization – “Putting it all together”it all together”

1n =31n =3

2n = 62n = 6

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Replication of Replication of ChromosomesChromosomes

ReplicationReplication is is the process of the process of duplicating a duplicating a chromosomechromosome

Occurs Occurs prior to prior to divisiondivision

Replicated Replicated copies are called copies are called sister sister chromatidschromatids

Held together at Held together at centromerecentromere

Occurs in Occurs in InterphasInterphas

ee

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A Replicated A Replicated ChromosomeChromosome

HomologsHomologs (same genes, (same genes, different alleles)different alleles)

SisterSisterChromatidsChromatids(same genes,(same genes,same alleles)same alleles)

Gene XGene X

Homologs separate in meiosis I and Homologs separate in meiosis I and therefore different alleles separate.therefore different alleles separate.

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Meiosis Forms Haploid Meiosis Forms Haploid GametesGametes

Meiosis must Meiosis must reduce the chromosome number by halfreduce the chromosome number by half Fertilization then restoresFertilization then restores the 2n number the 2n number

from mom from dad child

meiosis reducesgenetic content

toomuch!

The right number!

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Meiosis: Two Part Cell Meiosis: Two Part Cell DivisionDivision

HomologsHomologsseparateseparate

Sister Sister chromatidschromatidsseparateseparate

DiploidDiploid

MeiosisMeiosis I I

MeiosisMeiosisIIII

DiploidDiploid

HaploiHaploidd

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Meiosis I: Reduction DivisionMeiosis I: Reduction Division

NucleusNucleus SpindleSpindlefibersfibers NuclearNuclear

envelopeenvelopeEarly Early Prophase IProphase I(Chromoso(Chromosome number me number

doubleddoubled))

Late Late ProphasProphas

e Ie I

MetaphaMetaphase Ise I AnaphasAnaphas

e Ie ITelophase Telophase I I (diploid)(diploid)

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Prophase IProphase I

Early Early prophaseprophaseHomologs Homologs pair.pair.Crossing over Crossing over occursoccurs.

Late prophaseLate prophaseChromosomes Chromosomes condense.condense.Spindle forms.Spindle forms.Nuclear envelope Nuclear envelope fragments.fragments.

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Tetrads Form in Tetrads Form in Prophase IProphase I

Homologous Homologous chromosomeschromosomes

(each with sister chromatids) (each with sister chromatids)   

Join to form aJoin to form a TETRADTETRAD

Called SynapsisCalled Synapsis

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Crossing-OverCrossing-Over Homologous Homologous

chromosomeschromosomes in a tetrad in a tetrad cross over cross over each othereach other

Pieces of Pieces of chromosomes chromosomes or or genes are genes are exchangedexchanged

Produces Produces Genetic Genetic recombinationrecombination in the offspringin the offspring

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Homologous Homologous Chromosomes During Chromosomes During

Crossing-OverCrossing-Over

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Crossing-over multiplies the already Crossing-over multiplies the already huge number of different gamete huge number of different gamete types produced by independent types produced by independent

assortmentassortment

Crossing-OverCrossing-Over

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Metaphase IMetaphase I

Homologous Homologous pairspairs of of

chromosomes chromosomes align along the align along the equatorequator of the of the

cellcell

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Anaphase IAnaphase I

Homologs separateHomologs separate and move to opposite and move to opposite poles.poles.

Sister chromatids Sister chromatids remain remain attached attached at theirat their centromerescentromeres.

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Telophase ITelophase I

Nuclear envelopes Nuclear envelopes reassemble.reassemble.

Spindle disappears.Spindle disappears.

Cytokinesis Cytokinesis divides cell divides cell into two.into two.

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Meiosis IIMeiosis II

Only one homolog of Only one homolog of each chromosome is each chromosome is present in the cellpresent in the cell.

Meiosis II produces gametes Meiosis II produces gametes with with

one copy of each chromosome one copy of each chromosome and thus one copy of each and thus one copy of each gene.gene.

Sister chromatids carry Sister chromatids carry identical genetic identical genetic

informationinformation. .

Gene XGene X

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Meiosis II: Reducing Meiosis II: Reducing Chromosome NumberChromosome Number

Prophase Prophase IIII

MetaphaMetaphase IIse II

AnaphasAnaphase IIe II

TelophasTelophase IIe II 4 Identical 4 Identical

haploid haploid cellscells

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Prophase IIProphase II

Nuclear Nuclear envelope envelope fragments.fragments.

Spindle forms.Spindle forms.

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Metaphase IIMetaphase II

Chromosomes align Chromosomes align along along equatorequator of cell of cell..

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Anaphase IIAnaphase II

Sister Sister chromatidschromatids separate and separate and move to move to opposite polesopposite poles.

EquatorEquator

PolePole

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Telophase IITelophase II

Nuclear envelope Nuclear envelope assembles.assembles.

Chromosomes Chromosomes decondense.decondense.

Spindle Spindle disappears.disappears.

Cytokinesis Cytokinesis divides cell into divides cell into two.two.

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Results of MeiosisResults of MeiosisGametes (egg & Gametes (egg & sperm) formsperm) form

Four haploid cells Four haploid cells with one copy of each with one copy of each chromosome chromosome

One allele of each One allele of each genegene

Different Different combinations of combinations of alleles for different alleles for different genes along the genes along the chromosomechromosome

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GametogenesisGametogenesis

OogenesisOogenesis or or

Spermatogenesis Spermatogenesis

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SpermatogenesisSpermatogenesisOccurs in the Occurs in the

testestestesTwo divisions Two divisions

produce 4 produce 4 spermatidsspermatids

Spermatids Spermatids mature into mature into spermsperm

Men produce Men produce about about 250,000,000 250,000,000 sperm per daysperm per day

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Spermatogenesis in the Spermatogenesis in the TestesTestes

SpermatiSpermatidd

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SpermatogenesisSpermatogenesis

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OogenesisOogenesisOccurs in the Occurs in the ovariesovariesTwo divisions produce Two divisions produce 3 polar 3 polar

bodiesbodies that die and that die and 1 egg1 eggPolar bodies die because of Polar bodies die because of

unequal division of cytoplasmunequal division of cytoplasmImmature egg called Immature egg called oocyteoocyteStarting at puberty, one oocyte Starting at puberty, one oocyte

matures into an matures into an ovum (egg)ovum (egg) every 28 daysevery 28 days

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Oogenesis in the OvariesOogenesis in the Ovaries

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OogenesisOogenesis

OogoniumOogonium(diploid)(diploid)

MitosisMitosis

PrimaryPrimaryoocyteoocyte(diploid)(diploid)

Meiosis IMeiosis I

SecondarySecondaryoocyteoocyte(haploid)(haploid)

Meiosis IIMeiosis II(if fertilization(if fertilizationoccurs)occurs)

First polar bodyFirst polar bodymay divide may divide (haploid)(haploid) PolarPolar

bodiesbodiesdiedie

Ovum (egg)Ovum (egg)

SecondSecondpolar bodypolar body(haploid)(haploid)

a

A

X

X

a

X

A X

a

X

a

X

MatureMatureeggegg

A

X

A

X

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Comparing Comparing Mitosis and Mitosis and

MeiosisMeiosis

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MitosisMitosis MeiosisMeiosisNumber of Number of

divisionsdivisions 1122

Number of Number of daughter cellsdaughter cells 22 44

Genetically Genetically identical?identical? YesYes NoNo

Chromosome #Chromosome # Same as Same as parentparent Half of parentHalf of parent

WhereWhere Somatic cellsSomatic cells Germ cellsGerm cells

WhenWhen Throughout Throughout lifelife

At sexual At sexual maturitymaturity

RoleRole Growth and Growth and repairrepair

Sexual Sexual reproductionreproduction

Comparison of DivisionsComparison of Divisions

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