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Last Workshop…
• ImageJ basics– Image manipulation– Setting scale– Making measurements– Counting particles
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Today’s Plan…
• Advanced Image Analysis– Working with “stacks”
• Where to get images– Finding existing images– Creating your own images
• Online exercises– SimpleScience.org
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Stacks
• A collection of multiple images packaged together into a single computer file
• Think of a deck of cards– X & Y dimensions are spatial– Z dimension can be:
• Spatial (depth)• Temporal (time - video)• Spectral (color)
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CHANNEL
DEPTH
TIME
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Where To Get Images…
• Use somebody else’s
• Create your own
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Online Image Archives
• http://www.goes.noaa.gov/• http://www.osei.noaa.gov/• http://www.nwas.org/committees/rs/nwasat.html• http://glovis.usgs.gov/• http://images.jsc.nasa.gov/• http://serc.carleton.edu/NAGTWorkshops/visuali
zation/collections/imagearchives.html
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Creating Your Own Image Stacks
• Most any camera can be used to create scientifically useful images– Still– Video
• In most cases, Z dimension will be time
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Still Cameras
• Whenever possible, use tripod• Digital images have date/time stamps• Stacks can be built up manually in ImageJ
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Using Video In ImageJ
• QuickTime movies are opened as stacks– Must have QuickTime 7 installed
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Joe PowChester F. Carlson Center for Imaging Science
Rochester Institute of Technology
