Isonet® L TT and Isonet® L TT BIONew mating disruption products

for the control of Lobesia botrana (Lepidoptera: Tortricidae)

Andrea LucchiDip. Scienze Agrarie, Alimentari e Agro-ambientali, University di Pisa

andrea.lucchi@unipi.it

IOBC-WPRS meeting of the Working Group on "Integrated Protection and Production in Viticulture",

Austrian Agency for Health and Food Safety (AGES), Vienna, Austria

Lobesia botrana & Mating disruption

MD with hand-applied dispensers is the most well-studied and widely used pheromone-

mediated control technique against grape berry moths in Europe.

In 2015: applied on 140,000 ha in European vineyards (3–4% of the total grapevine-

growing area).

Rak, BASF Isonet, Shin-Etsu Puffer, Suterra

Lobesia botrana & Mating disruption

We have been researching on L TT and LTT BIO(biodegradable) because they show advanced merits:

- Applied at half point sources (250 vs 500)

- Applied in less time

- Biodegradable (in one case)

- Aluminum free

• reservoir pheromone dispensers: 2 parallel

capillary tubes (Twin Tubes) filled with L. botrana

pheromone blend, and joined and sealed at ends

• No need to twist, only open and hang

• gap in the middle allows each dispenser to form a

loop easily and quickly deployed over the end of

spurs or by looping it around cordons

• difference between Isonet ® L TT and Isonet ® L TT

BIO: material of which the dispensers are made:

polyethylene for Isonet ® L TT and biodegradable

plastic for Isonet ® L TT BIO

Isonet ® L TT & Isonet ® L TT BIO - products description

L TT BIO

L TT

Shape and material of which

dispensers are made is of

sound importance for a correct

and season-long release of

pheromone.

Efficacy of both products in

controlling L. botrana on

grapevine has been evaluated

and verified in several field

trials.

This talk deals with

the field research

we carried out in a

Farm of Tuscany in

2014 and

Lobesia botrana trials 2014 - 2015Tuscany

• Test site location: Bolgheri, Livorno, Tuscany (Italy)

• Grapevine cultivars: Vermentino (2014), Cabernet Sauvignon (2015)

• Tested treatments:

Isonet ® L TT & Isonet ® L TT BIO - trial design

Treatment ProductApplied rate

(dispensers/Ha)

Control -

Isonet L TT Isonet® L TT200 (2014)

250 (2015)

Isonet L TT BIO Isonet® L TT BIO200 (2014)

250 (2015)

StandardGrower’s Standard (2014)

Isonet® L (2015)

* (2014)

500 (2015)

• Grower’s standard 2014 = 2 sprayings I gen Methoxyfenozide

III gen Chlorantraniliprole

Frequency:

1. at end of 1st generation (G1; May, full flowering)

2. at end of 2nd generation (G2; July, berry touch)

3. at harvest (G3; September)

Type:

• % infested flower clusters (G1) / bunches (G2 and G3)

• N. nests per flower cluster (G1) / bunch (G2)

• N. damaged berries per bunch (G3)

10 subplots within each treatment, big enough to allow for assessments on at

least 100 bunches per subplot, were selected.

10 subplots x 100 flower clusters per treatment (G1) and 10 x 50-100 bunches per

treatment (G2 and G3).

Isonet ® L TT & Isonet ® L TT BIOASSESSMENTS

Challenges to the success of Mating Disruption:

• overcome the border effects of immigration of mated females;

• prevent potential treatment interference between plots;

• population density.

For these reasons:

• sites cannot be split into conventional plots;

• replicated treatments are often unpractical.

Plot size for the pheromone treatments may be of significant size (e.g. several hectares)

in order to achieve reliable performance. Plot size for either untreated controls or

standard insecticide treatments do not have to be as large, but should, where possible

have comparable population densities.

Whilst replication is preferred where practical, trials may be unreplicated but a suitable

number of trials should be conducted to allow appropriate support of product claims.

EPPO Guideline PP 1/264(1) Mating disruption pheromones

Similar trials in Tuscany, Emilia Romagna, Sicily and Spain supervised by the

same research team.

Statistical analysis:

• Since a randomized block design does not apply to large plots required for

studies on mating disruption products, ten subplots per each treatment

were considered as replicates;

• Data were compared across Treatments using 1-way ANOVA followed by

Student-Newman-Keuls’ test for post-hoc comparison of means. Bartlett’s

test was used to test for homogeneity of variances.

Isonet ® L TT & Isonet ® L TT BIO – data analysis

L TT BIO 1

L TT BIO 2L TT 1

L TT 2

CONTROL

Crop: grapevineVariety: Vermentino

Location:Bolgheri (LI)

Total surf.17.5 ha

L TT 5 h

L TT BIO 5 ha

Control 7.5 ha

Dosage/ha :LTT 200 d/haLTT BIO: 200 d/ha

Damage assessment:

I° gen: 31 May

II° gen: 17 July

III° gen: 03 Sept

1,000 clusters/plot (G1)

500 clusters/plot (G2 + G3)

WIND

BOLGHERI PLOT 2014

1

32

111

1

5

4

Grower’s standard

Experimental area

0

10

20

30

40

50

60

70

18.3

25.3

1.4

8.4

15.4

22.4

29.4

6.5

13.5

20.5

27.5

3.6

10.6

17.6

24.6

1.7

8.7

15.7

22.7

29.7

5.8

12.8

19.8

26.8

2.9

9.9

average captures/trap

NO

MD

20

14

NO

MD

1 2

01

4

NO

MD

trap

cap

ture

sB

OLG

HE

RI P

LOT

20

14

L TT

BIO

1

Trap

cap

ture

sB

OLG

HE

RI P

LOT

20

14

Re

sults

20

14

0 2 4 6 8

10

12

14

16

24.3

31.3

7.4

14.4

21.4

28.4

5.5

12.5

19.5

26.5

2.6

9.6

16.6

23.6

30.6

7.7

14.7

21.7

28.7

4.8

11.8

18.8

25.8

1.9

average captures/trap

L TT

L TT

BIO

Gro

we

r's Sta

nd

ard

Co

ntro

l

ResultsBOLGHERI PLOT 2014

Results 2014

0,0

5,0

10,0

15,0

20,0

25,0

30,0

35,0

40,0

45,0

50,0

CONTROL L TT L TT BIO GROWER'S

STAND.

5,3 b

1,3 c 1,2 c

15,9 a

31,2 a

13,0 b9,8 b

26,6 a

22,2 a

6,4 b4, 2 b

19,8 a

% Infested flower clusters (G1) / bunches (G2 and G3)

G1

G2

G3

Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and

all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3

ResultsBOLGHERI PLOT 2014

Results 2014

0,00

0,05

0,10

0,15

0,20

0,25

0,30

0,35

0,40

0,45

0,50

CONTROL L TT L TT BIO GROWER'S

STAND.

0,05 b

0,014 c0,015 c

0,186 a

0,42 a

0,136 b0,108 b

0,326 a

0,432 a

0,082 b0,048 b

0,282 a

N. nests (G1 and G2) and

damaged berries (G3) per bunch

G1

G2

G3

Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and

all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3

Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and

all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3

Grower’s standard was sprayed with Prodigy (Methoxyfenozide, 400 ml/ha) against G1 and

all fields were sprayed with Coragen (Chlorantraniliprole, 150 mL/ha) against G3

Migliarini stradone

Cbs

56,8%

Migliarini 2001

Vm

55,20%

Pozzi al comune

Vm19,8%

Campone

Cbs

5,6%

Camillo

LTT1

LTT2BIO1

BIO2

% INFESTATION III GENERATION 2014 DESPITE 2 SPRAYINGS

WITH METHOXYFENOZIDE AND CHLORANTRANILIPROLE

Bettelli

Cbs

Belvedere

Cbs

S.Antonio

Mer

2,9%

2014 trial

2015 trial

25 ha in 6 plots plus

a small control

Crop: grapevineVariety: Cab. Sauvign. Location: Bolgheri (LI)

Total surface: 25 ha

Isonet L TT: 8.5 haIsonet L TT BIO: 7.8 haIsonet L : 7.2 haControl: 1,5 ha

Dosage/ha :

Isonet L TT: 250 d/haIsonet L TT BIO: 250 d/haIsonet L: 500 d/ha

Damage assessment:I° gen: 27 MayII° gen: 16 JulyIII° gen: 03 Sept

1,000 clusters/plot (1G)500 clusters/plot (2G + 3G)

BOLGHERI PLOT 2015

L TT 1

L TT 2

L TT BIO 1

L TT BIO 2

L 1

L 2

CONTROL

0

20

40

60

80

10

0

12

0

18.3

25.3

1.4

8.4

15.4

22.4

29.4

6.5

13.5

20.5

27.5

3.6

10.6

17.6

24.6

1.7

8.7

15.7

22.7

29.7

5.8

12.8

19.8

26.8

average captures/trap

NO

MD

20

15 N

O M

D tra

pca

ptu

res

BO

LGH

ER

I PLO

T 2

01

4-2

01

5

Trap

cap

ture

sB

OLG

HE

RI P

LOT

20

15

Re

sults

20

15

0 5

10

15

20

25

30

35

30.3

6.4

13.4

20.4

27.4

4.5

11.5

18.5

25.5

1.6

8.6

15.6

22.6

29.6

6.7

13.7

20.7

27.7

3.8

10.8

17.8

average captures/trap

L TT

L TT

BIO

LCo

ntro

l

ResultsBOLGHERI PLOT 2015

0,0

5,0

10,0

15,0

20,0

25,0

30,0

35,0

40,0

45,0

50,0

CONTROL L TT L TT BIO L

5,9 a

0,8 b 0,8 b 1,3 b

25,5 a

2,3 b 3,1 b5,1 b

19,2 a

2,0 c

8,6 b 7,8 b

% infested flower clusters (G1) / bunches (G2

and G3)

G1

G2

G3

ResultsBOLGHERI PLOT 2015

Results 2015

0,00

0,05

0,10

0,15

0,20

0,25

0,30

0,35

0,40

0,45

0,50

CONTROL L TT L TT BIO L

0,071 a

0,009 b 0,009 b 0,014 b

0,287 a

0,023 b 0,031 b0,051 b

0,474 a

0,034 c

0,222 b

0,172 b

N. nests (G1 and G2) and

damaged berries (G3) per bunch

G1

G2

G3

• Time required for application of L TT dispensers was 1 hour per ha compared

with 1.5 hs per ha of Isonet L

• In both years, Isonet® L TT and Isonet® L TT BIO resulted in almost complete

trap catches shut-down of L. botrana.

• G1, G2 and G3 L. botrana infestation was lower in Isonet ® L TT and Isonet ® L TT

BIO than in the control treatment.

• Efficacy of both products in controlling L. botrana was comparable to or higher

than that of the reference treatment. This was confirmed by the results

obtained in other Italian Regions and in Spain.

• Isonet® L TT and Isonet® L TT BIO can be considered valuable tools for effective

and less labour-intensive control of European grapevine moth.

Isonet ® L TT & Isonet ® L TT BIO trials

Summary

Thanks for listening

L TT team