019-040 S1-PS - chimie.uvt.rochimie.uvt.ro/awut_sc/awut/1622007/poster19.pdf · Isac St., Cluj-Napoca, ROMANIA .([email protected]) 2National R&D Institute of Isotopic and Molecular

The Annual International Conference of the Romanian Society of Biochemistry and Molecular Biology

TIMIŞOARA, September 6-8 , 2007

Posters

19

REACTIVE OXYGEN SPECIES IN PATHOLOGY.

EXPERIMENTAL AND CLINICAL STUDIES

C l a u d i a B o r z a 1 , R o d i c a M a t e e s c u 2 , E r i k a D e a k 2 , G a b r i e l a N e g r i s a n u 2 , G e r m a i n e S a v o i u 3

1Departament of Physiolgy, 2Department of 3rd Internal Medicine, 3Department of

Pathophysiology, University of Medicine and Pharmacy Victor Babes Timişoara,

Romania, Pta E. Murgu no 2, Mail: [email protected]

ABSTRACT

Objective: It was studied the antioxidant capacity decreasing, expressed by

superoxidedismutase (SOD) and reduced glutathione (GSH) in experimental and clinical

studies. Method: In experimental asthma we used an experimental model with Sprague-

Dawley male rats sensitized with ovalbumin. In clinical study were investigated

asthmatic patients and 40 patients with noninsulin-dependent mellitus diabetes (NIDDM).

Results: SOD - the control value was 10.7 UI/ml and in sensitized group

significantly decreased at 7.6 UI/ml (p<0.01). In asthmatic children group the SOD

value was 9.02 UI/ml, comparatively with 11.1 UI/ml - the control value (p<0.01). GSH -

the control value was 606 uM/l and in sensitized group significantly decreased at 325

uM/l (p<0.001). In asthmatic children group the GSH value was 272.2 uM/l,

comparatively with the control value: 432.2 um/l (p<0.001).In patients with mellitus

diabetes, SOD significantly decreased at 6.8 +/- 1.2 UI/ml (p<0.001, normal value, 10

UI/ml). GSH significantly decreased at 342 +/- 87 μM/l (p<0.001, normal value 600μM/l).

Conclusion: These parameters express the endogenous antioxidant capacity,

significantly decreased in sensitized animals, asthmatic children group and in diabetic

patients in correlation with oxidative stress intensity.



Posters

20

CARBOHIDRATE-DEFICIENT TRANSFERRIN AND

GAMMA-GLUTAMYLTRANSPEPTIDASE IN THE

EVALUATION OF ALCOHOL CONSUMPTION

B . B u m b a c i l a 1 , D a n i e l a I o n e s c u 2 , A d r i a n a K a y c s a 1 , C r i s t i n a D e h e l e a n 2 , E l e n a G a l c a 3

1University of Medicine and Pharmacy ”Victor Babes”, Biochemistry Department

2University of Medicine and Pharmacy ”Victor Babes”, Faculty of Pharmacy, Toxicology Department, E. Murgu no 2, Timisoara, 300041, Romania, [email protected]

3Lugoj Hospital, Department of Psychiatry

ABSTRACT

Aim: The objective of this study was to compare carbohydrate-deficient

transferrin and gamma-glutamyl transpeptidase (GGT) assays for the evaluation of alcohol

consumption. Methods: 165 patients (100 male and 65 female) were included in this

study. Patients were divided into five categories according to alcohol consumption:

category 1 included non-weaned patients drinking more than 30g/day for women and

more than 50 g/day for men, category 2 included relapse patients, category 3 included

moderate drinkers, category 4 included patients weaned less than one month, and

category 5 included patients weaned more than one month. A specifically standardized

questionnaire was used. Results: Sensitivity of CDT varied, depending on patient category,

from 36% to 96% versus 45% to 70% for GGT. Specificity of CDT varied from 72% to 90%

versus 21% to 61% for GGT. After one month of abstinence specificity of CDT was 65%

versus 18% for GGT. Conclusion: This study confirms that carbohydrate-deficient

transferrin is more accurate in predicting alcohol consumption compared with gamma-

glutamyl transpeptidase in alcoholic patients evaluation.



Posters

21

HPLC SEPARATION OF CAROTENOIDS FROM

PHYSALIS ALKEKENGI L. IN DIFFERENT STAGES OF

MATURATION

A n d r e a B u n e a , A d e l a P i n t e a , C a r m e n S o c a c i u

Department of Chemistry and Biochemistry, University of Agricultural Sciences and

Veterinary Medicine, Manastur 3-5, 400372,Cluj-Napoca, Romania;

[email protected]

ABSTRACT

Physalis alkekengi L. is an ornamental plant known as „chinese lantern” which

also produces edible fruits rich in carotenoids. The carotenoid content and profile of

Physalis sepals have been determined in different stages of maturation.

The carotenoid content in all samples was determined comparatively, using

spectrophotometric and HPLC-PDA analysis on RP-18 column. The total carotenoid content

in green sepals was 203.72 mg/kg dry weight, 945.08 mg/kg dry weight in yellow sepals

and 2269.92 mg/kg dry weight in orange ones. The major carotenoid content was found in

orange sepals of Physalis alkekengi L. at full ripeness.

Chromatographic separation of the unsaponified extract by HPLC showed the

presence of β-cryptoxanthin monoesters and zeaxanthin diesters at more than 85% in the

yellow and orange sepals while in green ones they are totally absent. Other carotenoids

found were: neoxanthin, violaxanthin, antheraxanthin and unesterified β-cryptoxanthin

and zeaxanthin. The common carotenoid found in all stages of maturation was β-carotene.

The LC-MS analysis confirmed that β-cryptoxanthin and zeaxanthin are esterified only with

middle chain saturated fatty acids, mainly palmitic acid. The carotenoid esters biosynthesis

starts in yellow sepals and rich the full concentration in the orange sepals. Physalis

alkekengi L. is a valuable source of carotenoids and especially of zeaxanthin.



Posters

22

THE EFFECT OF A PROPOLIS EXTRACT

ADMINISTRATION IN CADMIUM INDUCED

OXIDATIVE STRESS, IN RATS

I u l i a n a C h i s - B u i g a , L u c i a O l a r i u , M i h a e l a P u p

Faculty of Veterinary Medicine, Timişoara 119-Calea Aradului, 300645-Timisoara,

ROMANIA [email protected]

ABSTRACT

The experimental oxidative stress due to a single doze administration of cadmium

(20 ppm cadmium as cadmium chloride) and the protective antioxidant action of an

alcoholic propolis extract was studied. The following parameters: malondialdehide,

catalase, superoxid dismutase, glutathione peroxidase, glutathione reductase, glutathione

and the liver and kidney cadmium concentrations were investigated.

The obtained results revealed that a single doze of 20 ppm of cadmium could

generate a significantly increasing of malondialdehide and of the superoxid dismutase

activity. Decreasing of glutathione peroxidase, glutathione reductase and catalase

activities were registered. The cadmium concentration in liver and kidney was significantly

lower after a propolis extract administration.



Posters

23

EVALUATION OF SOD AND GPX ACTIVITIES IN

SCHIZOPHRENIA

E l e n a C r i s t i n a C răc i u n 1 , M a r i a D r o n c a 2 , B o g d a n N e m eş 3 , D o i n a C o z m a n 3 1Pharmaceutical Biochemistry and Clinical Laboratory Department, Faculty of

Pharmacy;

2Medical Biochemistry Department, Faculty of Medicine;

3 Clinical Psichology and Mental Health Department, Faculty of Medicine;

„Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca

[email protected]

ABSTRACT

In recent years there is great evidence that oxygen free radicals play an

important role in the pathophysiology of many neuropsychiatric disorders. In

schizophrenia, antioxidant status could be altered as a consequence of both the evolution

of the disease and the neuroleptic treatment. In the present study we investigated the

activities of erythrocyte superoxide dismutase (SOD) and whole blood glutathione

peroxidase (GPx) in 70 schizophrenic patients and 43 healthy volunteers, age and sex

matched. We have observed significantly higher values of SOD activity (1486.45 ± 262.59

U/g Hb versus 1393.92 ± 250.73 U/gHb, p< 0.05) but normal values of GPx activity

(38.86 ± 9.59 U/g Hb versus 44.24 ± 11.53 U/g Hb, p>0.05) in the schizophrenia group

compared with the control group. These results suggest an adaptative response to the

increased superoxide radicals production.



Posters

24

OXIDATIVE EFFECTS AFTER PHOTODYNAMIC

THERAPY IN RATS

D o i n a D a i c o v i c i u 1 , A d r i a n a F i l i p 1 , S i m o n a C l i c h i c i 1 , S o i m i t a S u c i u 1 , A d r i a n a M u r e s a n 1 , N i c o l e t a D e c e a 1 , S i m i n a D r e v e 2

1Physiology Department, Medicine and Pharmacy University Cluj-Napoca 13 Emil

Isac St., Cluj-Napoca, ROMANIA .([email protected])

2National R&D Institute of Isotopic and Molecular Technologies, Cluj-Napoca

ABSTRACT

The oxidative effects of photodynamic therapy with 5-aminolevulinic acid (5-ALA)

and chitosan were evaluated in Wistar rats s. c. inoculated with Walker carcinoma. The

animals were irradiated with red light (λ=685 nm, D=50 J/cm2, 15 min) 3h after i.p

administration of 250 mg/kg b.w. 5-ALA or 1.5-mg/kg b.w. chitosan. The lipid peroxides,

protein carbo-nyls, -SH groups and antioxidant capacity (DPPH test) were determined in

blood and tu-mor tissues. 24h after the treatment, in 5-ALA treated animals the level of

lipid peroxides in tumors was increased as compared to controls (0.75±0.24 vs. 0.38±0.09

nmoles MDA/mg protein, p<0.05). Protein carbonyls had a higher level in tumor tissues of

5-ALA group comparatively to controls and chitosan group (6.18±3.00 vs 1.55±0.42 and

1.92±0.09 nmoles/mg protein). Chitosan had an antioxidant effect expressed by lipid per-

oxides level (0.22±0.09 nmoles MDA/mg protein). Our results suggest that photodynamic

therapy is associated with biomolecules alterations produced by oxygen reactive species.



Posters

25

IN VITRO AND IN VIVO TESTS TO EVALUATE OF THE

EFFICACY AND TOXICITY OF BETULINIC ACID

STANDALONE OR MIXED WITH HPGCD

C r i s t i n a A . D e h e l e a n 1 , C . Ta t u 1 , C o d r u ţa Şo i c a 1 , A l e x a n d r a G r u i a 1 , V. O r d o d i 1 , S . C î n tă - P î n z a r u 2 , A d r i a n a K a y c s a 1

1University of Medicine and Pharmacy „Victor Babeş” Timişoara, Square Eftimie Murgu no.2, 300041, Timişoara, e-mail [email protected] 2Babes Bolyai University, Faculty of Physics, Molecular Spectroscopy Department, Kogalniceanu 1, RO 400084 Cluj-Napoca, Romania

REZUMAT

Betulinic acid is a naturally occurring pentacyclic triterpene, intensive analysed for

its anticancer selective effects on some tumour cells (e.g., melanoma cells). Its

mechanism of action consists in inducing selective apoptosis. The objective of the present

study was to analyze the antitumour effect of betulinic acid standalone or mixed with

HPGCD (hydroxypropyl gamma cyclodextrin) as antileukaemia agent. Its activity was

determined on K562 erythroleukaemia cell line. The antitumour activity was also analyzed

from the angiogenic activity point of view. The toxic activity was appreciated on human

mesenchymal stem cells. In vivo tests consisted in the analysis of the betulinic acid

solution, starting from 12,5 mg/ml on embryonated egg model. Materials and methods

were specific for the in vitro and in vivo evaluations: MTT assay, mycroscopic evaluation

plus spectral analysis. The dissolution of the compounds in an aqueous environment

(serum) was achieved by adding of HPGCD 1:2 ratio as was indicated in literature data.

DMSO was used for dissolution for in vitro tests. The conclusions are that betulinic acid is

active in low doses on the tested tumour cells and has a reduce toxicity. It induces a dose-

dependent anti-angiogenic effect on the embryonated egg.



Posters

26

ANTIOXIDANT PROTECTION IN AUTISM

M . D r o n c a 1 , E . C . C răc i u n 2 , S . P. P a s c a 1 , F. I f t e n e 1 , I . B e n g a 1 1Faculty of Medicine; 2Faculty of Pharmacy, „Iuliu Haţieganu” University of Medicine

and Pharmacy Cluj-Napoca ([email protected] )

ABSTRACT

Autism is a severe neurodevelopmental disorder defined by social and

communication impairments and stereotyped behaviors. The underlying etiology of autism

is unknown. It is suggested that autism may result from an interaction between genetic,

environmental, and immunological factors, with oxidative stress as a mechanism linking

these risk factors. Knowing that the brain is highly vulnerable to oxidative stress due to its

limited antioxidant capacity and higher amounts of lipids and iron, we investigated the

activity of the erythrocyte superoxide dismutase (SOD) and whole blood glutathione

peroxidase (GPx) in patients with typical and atypical autism. We investigated 41 children,

21 with typical autism (age 4.82 ± 0.33 years) and 20 with atypical autism (age 8,99 ±

0.81 years) followed at the Pediatric Psychiatry and Pediatric Neurology Clinics in Cluj-

Napoca. The control children were free of any neuropsychiatric disorder, age and sex

matched. We observed normal level of GPx activity in both typical (44.66 ± 2.92 U/g Hb)

and atypical (42.02 ± 2.34 U/g Hb) autistics in comparison with age matched controls

(47.63 ± 3.30 U/g Hb and 44.23 ± 2.74 U/g Hb respectively). On the other hand, the

activity of SOD was significantly decreased only in children with atypical autism compared

with matched age controls (1154.28 ± 79.62 U/g Hb versus 1395.10 ± 60.18 U/g Hb, p<

0.05). In conclusion, our results indicate that the antioxidant defense is altered in atypical

autism, but not in typical autism.



Posters

27

THE TOTAL ANTIOXIDANT STATUS IN

HYPOTHYROIDISM

D . F i r u , S t e f a n a P o p e s c u , D a n i e l a Ta c h e , A n a K i e s e

Str. Petru Rares, no. 4, 200349, Craiova, Dolj [email protected]

ABSTRACT

The importance of the iodine ion oxidation to elementary iodine during

thyroidian hormone biosynthesis was well established . The iodine ion deficiency may

produce oxidative stress conditions by the increase of H2O2 concentration .The iodine

excess of thyroidian cells determines cytotoxicity dependent on the formation of free

radicals .These cytotoxic effects may depend on the total antioxidant status. In our

reaserch we watched the oxidative stress by lipidic peroxide products – malonil dialdehida

(MDA)-and the total antioxidant status for a group of hypothyroidian patients in the

Endocrinology Clinic of The University Hospital of Craiova .



Posters

28

THE INFLUENCE OF CULTIVATION CONDITIONS

UPON COQ PRODUCTION BY YEASTS

L u m i n i t a G e o r g e s c u a n d A n c a N i c o l a u

University „Dunarea de Jos” Galati, 47 Domneasca St., 800008 Galati,

[email protected] and [email protected]

ABSTRACT

Among microorganisms, yeasts are renamed for producing compounds from the

coenzyme Q group, but their use as CoQ10 industrial producers is not very popular

because of the bioprocess cost. In spite of this, natural CoQ is prefered to the synthetic

one as supplement in medicine, food and cosmetic products. In an attempt to improve the

CoQ10 bioproduction, three yeasts were investigated in our laboratory. The yeasts used in

the experiments were Saccharomyces cerevisiae from Pakmaya, a Candida sp. isolated

from wine and a Rhodotorula sp. isolated from fruits. The yeasts were cultivated on a

minimal medium based on sucrose and amonium salts, both under stationary and shaked

conditions in order to evaluate the importance of aeration in CoQ biosynthesis. Then, the

influence of different substances (hydrogen peroxide, cysteine, carrot juice, molasses)

upon CoQ10 production was tested. In order to extract the CoQ, yeast biomass was

submitted to repeated cycles of freezing-thawing. Coenzyme Q quantified by HPLC in n-

propanol extracts was better synthetised by Rhodotorula sp. Reverse-phase HPLC was

performed on an Agilent 1200 series system with a multiple wavelength detector under

isocratic conditions. The separation of CoQ was performed on a PRP-1 column with a

mixture of acetonitrile and tetrahydrofuran as mobile phase. CoQ retention time was 3.97

at 1 ml/min flow rate and 30°C.



Posters

29

THE DEPENDENCE OF ANTIOXIDANT ACTIVITY SEA

BUCKTHORN OILS UPON FATTY ACIDS

COMPOSITION

A l e x a n d r a T. G r u i a 1 , 2 , M a r i a n a A l b u l e s c u 1 , C a l i n A . Ta t u 2 , V a s i l e O s t a f e 1 1West University of Timisoara, Faculty of Chemistry-Biology-Geography, Department

of Chemistry, 16 Pestalozzi, Timisoara, 300115, Romania

2Transplant Immunology Department, Clinical Laboratory, County Hospital, Bd. Iosif

Bulbuca No.156, 300736 Timisoara, Romania

ABSTRACT

Sea buckthorn plants contain an array of nutrients with antioxidant activity, used

in medicinal purposes, as carotenoids, vitamins, phytosterols, flavonoids, etc. By its rich

content in essential fatty acids, different parts of berries are used to obtain the oil. Due to

its powerful antioxidant effects, the oil has topical applications in medicine. The aim of the

study was to establish the content of fatty acids from two different sea buckthorn oils

pharmaceutical products, and to evaluate their antioxidant capacity. In order to perform

the analysis, the samples were derivatized prior to injection in gas-chromatograph (GC)

and the compounds were identified with mass spectrometry (MS). The antioxidant activity

was evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Analysis of phases,

estherified and saponified, of sea buckthorn oils revealed a difference in the content and

structure of fatty acids from assayed samples. Comparing the results, one can establish

which oil is richer in polyunsaturated fatty acids and thus, better in radical scavenging

activity.



Posters

30

IRON SUPPLEMENTATION IN HEMODIALYSIS -

INFLUENCE ON OXIDATIVE STRESS PARAMETERS

M a n o l e s c u B , S t o i a n I , C a p u s a C , M i r c e s c u G , L i x a n d r u D , A t a n a s i u V, C o l t a n L , G a m a n L

University of Medicine and Pharmacy "Carol Davila", Bucharest

ABSTRACT

Oxidative stress in hemodialysis is considered to be the result of metabolic

changes in chronic renal failure, loss of low weight antioxidants during hemodialysis

session, biological incompatibility of the dialitic system and medication (especially

parenteral iron used to treat renal anemia). Iron administration can amplify oxidative

stress, most of the pharmaceutical preparations having loosely bound iron. From a

theoretically point of view arterial iron administration can be less harmful compared with

currently clinical venous administration. We have considered of interest to study the

influence of the two different routes of iron administration on erythrocyte antioxidants.

Superoxide dismutase, catalase, glutathione peroxidase and transferase activity and the

level of total and non proteic thiols were analyzed on 20 hemodialised patients receiving

parenteral iron therapy. In order to gain information on others antioxidants present in

these cells we have also determined TEAC on erythrocytes lysates. There were no

statistically significant differences between the levels of these parameters when iron was

administered through the arterial or venous route with the exception of non-proteic thiols

and TEAC wich were higher for arterial iron administration suggesting a possible benefit of

this route In order to definitely clarify if there is or there is not a marked difference for the

arterial versus venous iron administration in these patients we need to study also the

plasmatic variations of oxidative stress parameters.



Posters

31

MODIFICATION OF HSP 70 AND HSP 27 EXPRESSION

AND OXIDATIVE STRESS IN MANGANESE TREATED

HUH7 CELLS

M u n t e a n u M a r i a C r i s t i n a , P o s t o l a c h e L a u r a , B o d e a L . , C o s t a c h e M a r i e t a , D i n i s c h i o t u A n c a

University of Bucharest, Biochemistry and Molecular Biology Research Center, Spl. Independentei, 91-95, Bucharest, Romania, [email protected]; [email protected];

ABSTRACT

High levels of manganese are toxic for a number of tissues such as liver, brain, gills and different cell cultures. Prolonged Mn(II) exposure induces the formation of reactive oxygen species leading to impairment of the antioxidant system.The aim of the present study was to evaluate the HUH7 cells adaptative response to experimental manganese exposure by biochemical and molecular methods, with particular attention to oxidative metabolism and heat shock protein gene expression. Antioxidative enzymes (catalase, superoxide dismutase) changed their profiles after 12 and 18 hours of 5mMMn(II) treatment. The total SOD specific activity was increased possibly because of a higher level of generated superoxide anion and of increase in Mn–SOD expression that was accompanied by an elevation of its activity. Hsp27 and Hsp 70 protect the cells against toxic effects triggered by a variety of stimuli including oxidative stress. More specifically, it was shown that Hsp 27 reduced the production of reactive oxygen species. It seems that this protein could modulate the intracellular redox state. Using Real Time PCR, we have shown a significantly increased Hsp 27 mRNA expression after 2 hours of treatment with 5mM Mn(II) on Huh7 cells, whereas the level of Hsp70-1B mRNA increased immediately after the exposure (0.5 hr). Our results revealed that, the transcription of hsp70-1B and hsp27 at high levels occurred in the first 2 h after manganese chloride treatment. Later the transcription rate decrease, but is higher for hsp 70-1B than hsp27.



Posters

32

P53 AND RB (RETINOBLASTOMA) GENE

EXPRESSION IN TUMORAL CELLS, UNDER THE

INFLUENCE OF VITAMIN E

A . - M . N i c u l e s c u ( 1 ) , L . G . G h e t e a ( 1 ) , R . M o t o c ( 1 ) , G r. M i h a e s c u ( 1 ) , R . H u c u l e c i ( 2 ) , C . D i a c o n u ( 3 ) , C . U r s a c i u c ( 4 ) , G . S a v i ( 4 )

(1)Institute of Genetics, University of Bucharest, Romania, (2) Multiple Users Research Base – Molecular Biology, University of Bucharest, Romania, (3) Institute of Virology “Stefan S. Nicolau”, Bucharest, Romania, (4) National Institute for Research & Development in Pathology and Biomedical Sciences “V.Babes”, Bucharest, Romania, [email protected]

ABSTRACT

The aim of this study was to establish the in vitro and in vivo effects of vitamin E

(vit E) on Wistar rat Walker sarcoma 256 ascitic cells. The treatments with vit E were

administrated in different doses and time intervals. The analyses were performed by flow

cytometry, for the study of apoptosis dynamics, and by Real-Time PCR, for p53 and rb

gene level expression detection. The results obtained revealed that in vitro treatment with

vit E had the most significant result on the number of apoptotic cells after 48 hours of

treatment, when the maximum percentage of apoptosis was obtained (24,14%), while the

control culture presented a percentage of 3.45% of apoptotic cells. Real-Time PCR analysis

showed that the expression level increased for both genes, at two concentrations of the vit

E used in the experiments, indicating a increased transcription activity for p53 and rb. The

most significant result, for the in vivo experiments, was an increase of the life span of the

rats treated with vit E, but, in this case, none of the two genes (p53 and rb) level was

modified. This results suggest that, in vivo, vit E could have a stimulating effect mainly on

the immune system which become more efficient in decreasing the tumor progression

rate.



Posters

33

STUDY OF DEUTERIUM DEPLETED WATER EFFECT

IN EXPERIMENTAL CADMIUM CHLORIDE INDUCED

OXIDATIVE STRESS IN FEMALE RATS

L u c i a O l a r i u , M i h a e l a P e t c u , I u l i a n a C h iş - B u i g a , C a m e l i a T u l c a n , M i h a e l a P u p , I l e a n a B r u d i u

Faculty Of Veterinary Medicine, Timişoara, 119-Calea Aradului, 300645-Timisoara,

ROMANIA ([email protected])

ABSTRACT

The present work deals with the study of the deuterium depleted water (DDW)

effect on female rats with cadmium induced oxidative damages (20 ppm Cd/ b.w. in single

dose administration). The deuterium concentration in surface water is about 150 ppm

(over 16 mM) and more than 10 mM in living organisms. The possible role of the naturally

occurring deuterium in biological systems was not studied before 1993, when the first

experiments with DDW revealed that due to deuterium depletion the non-tumorous L292

fibroblast cells required longer time to multiply in vitro and DDW caused human breast

tumor regression in mice. In our study there was determined that in a short time

treatment (30 days) DDW has a prooxidant effect (malondialdehide average values are

increasing, glutathione are decreasing); the antioxidant system response was evident.

After Cd intoxication and 61 days treatment the superoxid dismutase and glutathione

reductase activities were stimulated. The glutathione-peroxidase registered decreased

activity values as controls.



Posters

34

IN VITRO ANTICANCER ACTIVITY OF THE EXTRACT

OF HORSE CHESTNUT FOLIAR BUDS, AESCULUS

HIPPOCASTANUM L.

C a m e l i a P e e v 1 , C r i s t i n a D e h e l e a n 1 , A d r i a n a K a y c s a 2 , C o d r u t a S o i c a 1 , 1University of Medicine and Pharmacy “Victor Babes”, Faculty of Pharmacy, P-ta

Eftimie Murgu No.2, Timisoara 300041, Romania, [email protected]

2 University of Medicine and Pharmacy “Victor Babes”, Department of Biochemistry,

P-ta Eftimie Murgu No.2, Timisoara 300041, Romania

REZUMAT

The horse chestnut foliar buds are a new category of vegetal products that are

used in in the modern phytotherapy, also called gemmotherapy. The natural compounds,

present in small quantities, develop a synergic anticancer activity. In this study we have

determined the water content, total nitrogen, proteins, hemolytic saponins and the

presence of esculin by TLC. The anticancer activity of the crude extract was demonstrated

by the comparative biotest on Lepidium sativum, and, in vitro, on neuroectodermal cell

types from skin carcinoma (A431) and breast cancer cells (MCF 7).



Posters

35

FUNCTIONAL ANALYSIS OF FOUR SELECTED

C/EBPα GENE PROMOTER POLYMORPHISMS

PREVIOUSLY ASSOCIATED WITH FEATURES OF THE

INSULIN RESISTANCE SYNDROME/T2D

C o r i n a S a m o i lă , L i v i u Tăm aş , E d w a r d Şe c l a m a n , A d r i a n a N e g h i nă Biochemistry Department, University of Medicine and Pharmacy “Victor Babes”, Timisoara, [email protected]

ABSTRACT

The CCAAT / enhancer binding protein alpha, CEBPα, is a member of CEBP family

transcription factors. All members (C/EBPα-C/EBPζ) contain basic leucine zipper domain

(b-ZIP), in the C-terminus region, involved in DNA binding and dimerisation. Multiple roles

of the C/EBP family were identified in the control of cell cycle and differentiation processes,

in metabolism and inflammation. Studies have demonstrated that the CEBPα plays a key

role in the process of adipocytes differentiation. A new interest has been developed in the

analysis of associations of the CEBPα with the development of the insulin resistance

syndrome/T2D. Several common polymorphic variants of CEBPα promoter associated with

haemeostatic and metabolic factors related to the insulin resistance syndrome/T2D were

previously identified in a region spanning 3 kb from CEBPα promoter. In this study, using

stable transfections of the 3T3-442A cells (mouse preadipocyte cell line) with polymorphic

and wild type promoter constructs, we measured activities of CEBPα promoters to asses

the effects of four haplotype constructs on gene transcription in order to identify functional

sites important for regulating the level of CEBPα gene transcription in 3T3-F442A CEBPα-

expressing cell line. Analyses of a series of promoter constructs containing the four gene

haplotypes, either in preadipocytes or differentiated adipocytes, showed significant higher

activity of one promoter construct comparative with the others.



Posters

36

TRANGENOMIC WAVE™ DNA FRAGMENT ANALYSIS

OF A C/EBPβ GENE PROMOTER FRAGMENT

C o r i n a S a m o i lă a , A n d r e i A n g h e l a , A l f a X e n i a L u p e a b , M a r i l e n a M o t o c a a Biochemistry Department, University of Medicine and Pharmacy “Victor Babes”,

Timisoara, [email protected]

bBiochemistry Department, University of Polytechnics, Faculty of Engineering and

Environmental, Timisoara

ABSTRACT

Functional analysis of human promoter provides information about the gene

regulation mechanisms and the molecular networks involved. In this study, we have

investigated the association of C/EBPβ and atherosclerosis, using Trangenomic WAVE™

DNA Fragment Analysis on a fragment of 248bp from C/EBPβ gene promoter, to identify

the gene promoter polymorphisms possible associated with atherosclerosis and other

cardiovascular risks factors in 144 subjects with significant atheroma (1,2 or 3 major

coronary arteries with >50% obstruction, and/or peripheral artery lesions and/or at least

one atheromatous carotid stenosis demonstrated by angiography and echography) and in

150 subjects with no significant atheroma. Analysis revealed two adjacent polymorphisms

-411/-412 CG /TT. No heterozygous individuals for each single polymorphism were

distinguished. There was no difference in the genotype distributions of patients with

significant atheroma (haplotypes frequencies: -411/-412 CG [30.5%], -411/-412 CGTT

[50.7%], -411/-412 TT [18.8%]) and without significant atheroma (haplotypes

frequencies: -411/-412 CG [28.9 %], -411/-412 CGTT [48.3%],-411/-412 TT[22.8%]).



Posters

37

DIABETES VS CHRONIC RENAL FAILURE -

OXIDATIVE AND CARBONYL STRESS

S t o i a n I . , C a p u s a C . , M i r c e s c u G . , A t a n a s i u , V. , M a r a c i n e , M . , V i r g o l i c i , B . , M u s c u r e l C . , L i x a n d r u D . , M a n o l e s c u B . , G a m a n L .

University of Medicine and Pharmacy "Carol Davila", Bucharest

ABSTRACT

Advanced glycation end products (AGE) have been found increased in blood

samples from both diabetes and chronic renal failure patients suggesting a relationship

between carbonyl stress (accumulation of substrates due to excess production or impaired

clearance) and oxidative stress. Because both conditions have similarities regarding the

presence of AGE but different causes for this presence we have considered of interest to

study parameters of carbonyl and oxidative stress in diabetic and chronic renal failure

patients.We have selected 18 well controlled type II diabetes patients, 20 hemodialysed

patients with diabetes, 20 hemodialysed patients not having diabetes and 22 age matched

control subjects. On fasting blood samples we have determined routine biochemical

parameters as well as dicarbonyl compounds, Amadori Products (precursor of AGE),

TBAR'S, TEAC, antioxidant "gap" and total plasmatic thiols. In diabetes patients the

presence of oxidative stress is suggested by increased TBAR'S. In these patients there is

no difference from the controls for the level of TEAC antioxidant "gap" and total thilos. The

carbonyl stress markers studied (dicarbonyl compounds and Amadori Products) are also

not statistically significant different from the controls. For chronic renal failure patients

with end renal disease treated by hemodyalisis, with or without diabetes, we have put in

evidence the presence of both oxidative and carbonyl stress, the parameters studied being

significantly different from the controls, the patients suffering from both diabetes and

chronic renal failure looking the most vulnerable.



Posters

38

THE BIOMEDICAL APPLICATIONS OF THE MAGNETIC

NANOPARTICLES

M a r i a n a Ş i n c a i , D i a n a A r g h e r i e , D i a n a G â n gă , D o i n a B i c a

Faculty of Veterinary Medicine, Timişoara, 119-Calea Aradului, 300645-Timisoara,

ROMANIA, ([email protected])

ABSTRACT

Our researches about the biomedical applications of the magnetic nanoparticles

were directed in three domains. For the cicatrizing and anti-inflammatory effects

were experimented three types of magnetic fluids on skin lesions at guinea pigs, mice and

dogs and some volunteer persons. Comparatively, we used an existing pharmaceutical

product on control skin lesions. Periodically, from the experimental group animals, we

gather small skin fragments for histological study. Both the clinical and the histological

exams pointed out that the magnetic nanoparticles had an anti-inflammatory and rapid

cicatrizing effect. For the antitumoral effect of the biocompatible magnetic fluids, the

experiments were experimented on bitch and cats mammary tumors and fibro sarcoma.

The best results were obtained using the magnetic fluid based on water with magnetite

nanoparticles and with the magnetic fluid based on water with cobalt ferrite nanoparticles.

In all studied cases, we observed that in the first week the evolution of the tumors was

stopped. After that, step by step, the tumors reduced in size. To point out the UV-

protector effect were experimented three types of magnetic nanocompounds on white

mice auricles. The experimental animals were exposed at an UV lamp. After 8 hours it was

observed that the mice auricles protected with nanocompounds was well protected by UV-

radiation comparatively with control auricles at which appeared a severe erythema.



Posters

39

SOD ACTIVITY IN FRESH AND THAWED SEMINAL

PLASMA AFTER CRYOPRESERVATION USING TWO

DILUENTS

T u l c a n C . 1 , C s e h S . 2 , C h i s u I . 1 , O l a r i u L . 1 , I g n a V. 1 , C e r n e s c u H . 1 1Faculty of Veterinary Medicine, 119 Calea Aradului, 300645 Timisoara , e-mail:

[email protected]

2SzIU, Faculty of Veterinary Science Budapest, Hungary

ABSTRACT

The objective of this study was to characterize superoxide dismutase activity

associated to cryopreservation and its implications on the quality of the dog semen

cryopreserved with two different diluents: Biladyl and Bioxcell.

Twenty-five ejaculates collected from 8 males aged from 1 to 2½ years, were

used in this study. The cryopreservation was performed using the Biladyl and Bioxcell

protocols. The SOD activity was evaluated in fresh and cryopreserved seminal plasma by

NBT method. The quality parameters of semen (motility, viability and morphology) were

evaluated using WHO techniques.

In cryopreservation process, a decrease of the SOD activity is recorded,

compared to that of the fresh seminal plasma. The release of the intracellular SOD and the

onset of the oxidative stress are evidenced in both cases. The fact that the injury level of

the spermatozoa plasmatic membrane integrity has a decisive role on the level of the SOD

activity after thawing is demonstrated in the case of Bioxcell diluter, where the worse

quality of semen was registered.



Posters

40

SPECTROPHOTOMETRIC STUDY ON THE

RIBOFLAVIN − PLANT EXTRACTS INTERACTION

M a r i a n a V o i c e s c u 1 , L i l i a n a L u n g u 2 , V a s i l e D i n o i u 2 , M a r i l e n a V a s i l e s c u 1

1Romanian Academy, Institute of Physical Chemistry „Ilie Murgulescu”, Splaiul Independentei 202, 060021 Bucharest, Romania ([email protected]) 2Romanian Academy Institute of Organic Chemistry „C.D.Nenitescu”, Splaiul Independentei 202B, 060021 Bucharest, Romania ([email protected])

ABSTRACT

Intrinsic radical scavenging processes of compounds administered with food

have to warrant metabolic control within certain limits. Antioxidants which in many cases

are free radical scavengers or quenchers of activated states comprise a wealth of classes

of organic molecules including phenolics, probably as the most prominent ones. Using

UV-VIS Absoption and Fluorescence Spectroscopy, in this paper mechanism of protection

from oxidative damage of some plant extracts are discussed. Moreover, examples of

antioxidative functions of a few important plant extracts in certain diseases are reported.

Riboflavin - Plant Extracts Interaction is investigated in order to get information if

riboflavin acts as additional sensitizer to the superoxide generation. The results are

discussed with relevance to the oxidative stress process that takes place in humans.

Documents

019-040 S1-PS - chimie.uvt.rochimie.uvt.ro/awut_sc/awut/1622007/poster19.pdf · Isac St., Cluj-Napoca, ROMANIA .([email protected]) 2National R&D Institute of Isotopic and Molecular