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BIOCH 590: Biomacromolecules, Part I Spring 2010

Copyright: Jianhan Chen

Main References: 1. Chapters 5 & 7 of van Holde 2. Chapters 6 & 7 of Tinoco

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Maximum free energy Minimum entropy

Minimum free energy Maximum entropy

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x 2 =x 2c(x,t)dt"c(x,t)dt"

=x 2e#x

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cytoplasm nucleus

dextran control Ref: Lukas et al, J. Biol. Chem. (2000)

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s =v

r" 2 =m #m0

f=

m(1# v $)f : partial specific volume

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Sedimentation Coefficients of common biological materials

(in Svedbergs)

1 S = 10-13 second

nucleic acids

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Maximum free energy Minimum entropy

Minimum free energy Maximum entropy

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DC

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(r 2"r02 )

Molecular weight determined by fitting the gradient!

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C(r) /C(r0) = em(1"v # )2kT

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Biochem. J. (1996) 320 (39–47) Screenshot of SEDPHAT fitting analysis of protein-DNA binding

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g(2)(") = i(t)i(t + " ) / i(t) 2

g(2)(") =1+ ce#2h 2D" , with h = 4$n%#1 sin(& /2)

t

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