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Patient Name: Jonathan Sandman Case Investigation #: 587291 Dear Investigators, We have decided that you should focus your next phase of the investigation on the patient’s family and, if needed, a more detailed DNA analysis. We are supplying you with a log of family sleep assessments that were sent out by our records department and completed by the patient’s extended family. This won’t be easy, as the Father was adopted and doesn’t have information on his family history, but we think we might be able to still get some insight from the Mother’s family. Look for any patterns or responses that indicate a sleep pattern similar to the one the patient is experiencing. Also, since we lack a family history for the patient’s father we have authorized a specific DNA sequencing test be run to compare the father and patient. Take a look at the results of this test and report back to us with your findings. Below is the list of materials we were able to gather for checkpoint C:

• Patient and family records o Returned sleep assesment surveys from mother’s side of

family o Family History Tree For Jonathan Sandman o Sleep Assessment- Personal Accounts o DNA sequencing results for father and patient

• Hospital Info Sheet: PCR and DNA Sequencing • Guidelines for Review of Chronotype Survey • DNA Cheek Swab Results

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Patient Name: Jonathan Sandman Case Investigation #: 587291 Patient Sample Compared to Father: Charles Sandman Below are the dye terminator sequences for Charles and Jon sandman released from the lab. The sequence for the Father is on top, compared to the patient (Jon Sandman) below:

Charles Sandman

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Patient Name: Jonathan Sandman Case Investigation #: 587291 Investigators, As mentioned, here are the procedure guidelines to help you complete the family sleep history. Attached is a basic family tree breakdown for the patient reconstructed from conversations with the patients mother and father. The father informed us that he was adopted and has no recorded family hisotry, but the mother indicated that several family members may have had some kind of modified sleep pattern. The mother’s family was very cooperatve in completing the surveys and providing us with the information we needed. For family members that are deceased or could not be reached to survey, information on estimated sleep pattern was collected from realatives. Below are the score ranges you should use to classify each family member by their chronotype (sleep pattern):

Score Range Chronotype

0-26 Not Affected-Patient does not show sufficient signs of an early morning chronotype

27-30+ Morning Type-Patient shows signs of a morning-shifted chronotype

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Figure 1: Steps in PCR

Figure 3: Steps of Dye-Terminator Sequencing

Figure 2: Fluorescent peaks detected by a sequencing

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Figure 4: Sequencing Machine

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