AM & LORD

Ph

arm

ace

uti

cal I

nd

ust

ry

Am&Lord Co.,Ltd.

Tel. +82-1566-7815Web. www.amenlord.co.kr

● SB-Injection

SB注射剂是以纯天然物为基础的注射剂，通过各种前临床测试和临床测试验证了稳定性并证明了抗癌效果，继而开发成具有优异功效的医药品。

产品的优点简单介绍如下：① 副作用极小的抗癌剂

(即使触及皮肤也不会有不良反应的安全的药物)② 对多种癌症有效③ 止疼效果 – 抑制晚期癌症患者的疼痛提高生活质量

如上所述，一般的抗癌药含有的毒性会导致很多副作用，但是SB注射剂不仅无毒，而且在没有重大的副作用下进行治疗。

从许多现代医学无法治疗的癌症患者通过SB注射剂得到治愈开始，在韩国得到了媒体和学术界的关注。它不仅有抗癌效果，还具有减少癌症患者的痛苦感的止痛效果，从而可以改善癌症患者的生活质量。

AM&LORD从纯天然抗癌剂领域逐渐扩展到食品，保健食品领域，从而成为也可以造福于健康人的公司。2020年，为了将纯天然物原料出口到中国，在堤川工业区构建了医药品生产厂(KGMP设施)，并在鸡龙工业区新建原料药生产厂(BGMP设施)和研究设施，为满足产品供给做准备。

(株)AM&LORD是研究开发纯天然抗癌剂和细胞治疗剂的公司。

● NK Cell Therapy

AM&LORD特有的免疫细胞治疗技术，比以前的免疫细胞培养方法更有效的增加并激活NK细胞，同时，培养之后，NK细胞占有比率也可显著增加。

即使最严重的晚期癌患者，不仅可以通过培养，增殖NK细胞，还可以使其活性最大化，它们对癌症治疗非常有效。

在NK细胞培养时获取的培养液，不仅对皮肤美白或皮肤再生都有非常优异的效果，还可以应用于治疗伤口及多种皮肤病，可以创造出创新未来价值。

● AM&LORD的纯天然抗癌剂和细胞治疗剂如下

★ 纯天然抗癌剂部分

用途 品目名 剂型 其它 保健区分

用于肺癌的吸入剂 肺诊飞 吸入剂 用于肺癌，肺炎的喷雾器 中药

肝癌，临床2期 SB注射 粉末注射剂 肝癌，腹膜转移癌2期进行中 处方药

抗癌，针药 癌诊飞 针药剂 固型癌 中药

抗癌 固型剂 癌诊飞2 胶囊 晚期癌患者 中药

动物药 抗癌剂 动癌飞 粉末注射剂 固型癌，动物医药品，宠物狗 动物医药品

★ SB注射的作用机理

(1) Inhibition the expression of HIF-1α and vascular endothelial growth factor in various cancer cells.

Figure.1. Effect of PKE(Pulsatilla koreana extract) on angiogenesis of Huh-7 cells.

(A) Expression of HIF-1α and (B) VEGF by PKE in hypoxia-induced Huh-7 cells

(CoCl2, 100μM). (C) Effects of PKE on tube formation in vitro.

HUVECs were plated on Matrigel(200μl/well) and treated with various concentrations of PKE. Capillary tube formation was assessed after 14h. Tube formation was observed under a phase-contrast microscope and photographed at x400 magnification.

(D) Effects of PKE on migration in vitro. HUVECs were plated at 90% confluence and a scratched was made with a razor blade. After wounding, the cells were washed with a serum-free medium and incubated in M199 with 5% FBS and 5 ng/ml bFGF, 1mM thymidine and/or PKE (100μg/ml). M199 medium with 2% FBS was used as negative control. Data represent the mean of at least three independent experiments done in triplicate.

★ Mechanism of SB injection

(2) Inhibition the c-Met/Akt/mTOR signaling pathway in various cancer cells

Fig.2. c-Met inhibition by SB365.

(A) Phosphorylation of RTKs by SB365 and expression of phospho-human epidermal growth factor receptor 2, phosphoepidermal growth factor

receptor and phospho-neurotrophic tyrosine kinase receptor by SB365 in MKN-45 gastric cancer cells.

(B) Proposed structure for the c-Met complex with the aglycone of SB365 (the original crystal ligand: green; the aglycone of SB365: gray). H-

bonding interactions between the aglycone of SB365 and c-Met are shown in green dotted lines. The DFG moiety of c-Met was indicated in a

magenta ribbon.

(C) Effects of SB365 in c-Met alteration cells.

The growth of the cells (MKN-45 gastric cancer cells; c-Met amplification cells, NCI-H596 non-small cell lung cancer cells; c-Met splicing

mutation cells and HT-29 colon cancer cells; c-Met overexpression cancer cells) were measured by the 3-(4,5-dimethylthiazole-2-yl)-2,5-

diphenyl tetrazolium bromide assay.

(D) Inhibition of p-Akt, p-STAT3 and p-Mek by SB365 in MKN-45 gastric cancer cells.

(E) Effect of SB36 on the c-Met/AKT/mTOR pathway in MKN-45 gastric cancer cells. The cells were treated with SB365 at various doses (0.1–10 μM).

Western blotting experiments for p-Akt, p-mTOR and p-p70S6K were performed with the cell lysates.

(F) Immunofluorescent imaging at × 400 magnification of c-Met/Akt/mTOR target proteins after treatment with SB365 is shown. Anti-rabbit

antibodies against p-Akt, p-mTOR and p-p70S6K were used for labeling. DAPI was used to counter stain the nucleus.

★ Mechanism of SB injection

(3) Effect of Pulsatilla koreana extract(PKE, SB Injection) on apoptosis of 8505c anaplastic thyroid cancer (ATC)

cells. (Expression of PARP, cleaved caspase-3, Bax and Bcl-2 were Expression of PARP, cleaved caspase-3)

Apoptosis, which is also known as programmed cell death, plays a

critical role in treating cancer(15). Caspases are a conserved family

of enzymes that bring cells to apoptosis. Of these, caspase-3 is one

of the key components of apoptosis, being responsible either

partially or completely for the proteolytic cleavage of various key

proteins, such as PARP, a protein repairing DNA and maintaining

genomic DNA integrity(16,17). Thus, the anticancer effects of PKE

were first investigated through the mechanism of apoptosis in

8505c cells. In this study, we observed that PKE increased the

expression of cleaved caspase-3 and PARP, leading to apoptotic cell

death. These apoptotic effects of PKE were confirmed by the results

of TUNEL and DAPI staining. The cells keep the homeostasis of the

anti-apoptotic regulators, including Bcl-2, and pro-apoptotic

regulators, such as Bax, to maintain the proper survival and

turnover. In this study, a dose-dependent increase of Bax and

decrease of Bcl-2 were observed in PKE-treated 8505c cells. These

results implied that PKE-induced apoptosis is likely to be an

important factor in the suppression of tumor growth.

★ Mechanism of SB injection

(4) 通过细胞周期分析，确认SB注射的G2/M阻滞诱导

不同浓度的SB注射用T98G处理之后，分析细胞周期，确认了S

B注射的浓度依赖性地在T98G中有G2/M phase的阻滞发生。不同浓度的SB注射用U87-MG处理之后，分析细胞周期，同样确认有

G2/M phase的arrest发生。

★ Mechanism of SB injection

(5) SB注射诱导自我吞噬

不 同 浓 度 的 SB 注 射 用 U87-

MG处理之后，在0，24，48小时的时候为了确认具有代表性的

autophagy( 自 我 吞 噬 ) 的 标 志 LC-

3的显现，通过荧光免疫染色确认，分布在细胞质上的LC-

3的显现，经过SB注射处理之后逐渐增加的同时还确认了autop

hagopore的形成也在增加。

不 同 浓 度 的 SB 注 射 用 U87-MG 处 理 之 后 ， 在 24,

48 小 时 的 时 候 提 取 蛋 白 质 ， 对 caspase-3 和 LC-3, p-

AKT/AK进行了免疫印迹。

其 结 果 确 认 了 ， caspase-3 没 有 观 察 到 cleavage ， LC-

3II 在 24 ， 48 小 时 时 从 200 μ g/ml 开 始 都 有 所 增 加 。 p-

AKT在24小时的时候400μg/ml，48小时时100μg/ml开始减少显现。

由此，可确认SB注射抑制AKT信号通路，通过自我吞噬来诱导neurobl

astoma U87-MG的细胞消亡。

● AM&LORD的纯天然抗癌剂和细胞治疗剂如下

★ 细胞治疗剂部分

产品名 规格 Contents

RKo-NK

NK

免疫细胞

治疗剂

NK-A 诱导免疫提高性，有助于自身免疫疾病及免疫力低下导致的无力感等

NK-B 担心有家族史而患癌症的人，需要提高免疫力的人，残留癌治疗

NK-C 癌症患者的积极治疗，需要较强免疫力的情况，建议医生的管理

NK-D癌症患者的积极治疗， 需要最强免疫力的情况，有可能发高烧，因此建议在医

生的管理监督下治疗

NK-E 积极诱导免疫提高性,有利于自身免疫疾病，免疫力低下导致的无力感

NK-E癌症患者的较积极的治疗，急需治疗的情况，在医院医生的监督下治疗

(day2手术)

→ 非常难以培养的NK细胞，以特殊的培养培养基容器模样开发，

使任何一个人都容易培养

→ 不需要Feeder cell或伽马射线检查，易可简单的培养

→ 即使是重症癌患者的免疫细胞也可以将其数量增殖500-2000倍以

上，为了最大程度提高RK-NK细胞的活性，找出各种抑制妨碍因素

的物质结合到技术中

→ 不同于其它技术，可以同时最大程度提高免疫细胞增殖和活性化

→ 将免疫抑制因子(immune suppressing factors)knockout的NK细胞

治疗剂

→ 用于异体NK细胞治疗剂的纯NK细胞也非常容易培养，而且开发一

种技术，即便包含T细胞可以用于异体细胞治疗的方法，不需要T细

胞分离工艺的技术。

★ AM&LORD 细胞治疗的优点

AM & LORD

Thank you

Ph

arm

aceu

tical In

du

str

y

Am&Lord Co.,Ltd.

Tel. +82-1566-7815Web. www.amenlord.co.kr