ヌクレオチド結合性酵素

LADH

GAPDH

アルコールデヒドロゲナーゼ

グリセルアルデヒド-3-リン酸

デヒドロゲナーゼ

ヌクレオチド結合ドメイン

α/β構造

乳酸デヒドロゲナーゼ

ヌクレオチド結合ドメイン

α/β構造

SER THR ALA GLY LYS VAL ILE LYS CYS LYS ALA ALA VAL LEU TRP GLU GLU LYS LYS PRO PHE SER ILE GLU GLU VAL GLU VAL ALA PRO PRO LYS ALA HIS GLU VAL ARG ILE LYS MET VAL ALA THR GLY ILE CYS ARG SER ASP ASP HIS VAL VAL SER GLY THR LEU VAL THR PRO LEU PRO VAL ILE ALA GLY HIS GLU ALA ALA GLY ILE VAL GLU SER ILE GLY GLU GLY VAL THR THR VAL ARG PRO GLY ASP LYS VAL ILE PRO LEU PHE THR PRO GLN CYS GLY LYS CYSARG VAL CYS LYS HIS PRO GLU GLY ASN PHE CYS LEU LYS ASN ASP LEU SER MET PRO ARG GLY THR MET GLN ASP GLY THR SER ARG PHE THR CYS ARG GLY LYS PRO ILE HIS HIS PHE LEU GLY THR SER THR PHE SER GLN TYR THR VAL VAL ASP GLU ILE SER VAL ALA LYS ILE ASP ALA ALA SER PRO LEU GLU LYS VAL CYS LEU ILE GLY CYS GLY PHE SER THR GLY TYR GLY SER ALA VAL LYS VAL ALA LYS VAL THR

190200210220230240250260270280290300310320330340350360370

5

Amino acid sequence of horse liver alcohol dehydrogenase10

GLN GLY SER THR CYS ALA VAL PHE GLY LEU GLY GLY VAL GLY LEU SER VAL ILE MET GLY CYS LYS ALA ALA GLY ALA ALA ARG ILE ILE GLY VAL ASP ILE ASN LYS ASP LYS PHE ALA LYS ALA LYS GLU VAL GLY ALA THR GLU CYS VAL ASN PRO GLN ASP TYR LYS LYS PRO ILE GLN GLU VAL LEU THR GLU MET SER ASN GLY GLY VAL ASP PHE SER PHE GLU VAL ILE GLY ARG LEU ASP THR MET VAL THR ALA LEU SER CYS CYS GLN GLU ALA TYR GLY VAL SER VAL ILE VAL GLY VAL PRO PRO ASP SER GLN ASN LEU SER MET ASN PRO MET LEU LEU LEU SER GLY ARG THR TRP LYS GLY ALA ILE PHE GLY GLY PHE LYS SER LYS ASP SER VAL PRO LYS LEU VAL ALA ASP PHE MET ALA LYS LYS PHE ALA LEU ASP PRO LEU ILE THR HIS VAL LEU PRO PHE GLU LYS ILE ASN GLU GLY PHE ASP LEU LEU ARG SER GLY GLU SER ILE ARG THR ILE LEU THR PHE

0102030405060708090100110120130140150160170180

5 10β1

β2

β3

β4

β5

β6

αA

αB

αD

αE

Zinc binding sitecatalyticnon-catalytic

ヌクレオチド結合ドメイン

約130から140アミノ酸で構成

Dogfish Muscle Lactate Dehydrogenase

ARG TYR LEU MET GLY GLU ARG LEU GLY VAL HIS SER CYS SER CYS HIS GLY TRP VAL ILE GLY GLU HIS GLY ASP SER VAL PRO SER VAL TRP SER GLY MET ASN VAL ALA SER ILE LYS LEU HIS PRO LEU ASP GLY THR ASN LYS ASP LYS GLN ASP TRP LYS LYS LEU HIS LYS ASP VAL VAL ASP SER ALA TYR GLU VAL ILE LYS LEU LYS GLY TYR THR SER TRP ALA ILE GLY LEU SER VAL ALA ASP LEU ALA GLU THR ILE MET LYS ASN LEU CYS ARG VAL HIS PRO VAL SER THR MET VAL LYS ASP PHE TYR GLY ILE LYS ASP ASN VAL PHE LEU SER LEU PRO CYS VAL LEU ASN ASP HIS GLY ILE SER ASN ILE VAL LYS MET LYS LEU LYS PRO ASN GLU GLU GLN GLN LEU GLN LYS SER ALA THR THR LEU TRP ASP ILE GLN LYS ASP LEU LYS PHE

5 10

αB

αD

αA

β3

β6

β5

β4

β1

β2

0102030405060708090100110120130140150160

αE

ALA THR LEU LYS ASP LYS LEU ILE GLY HIS LEU ALA THR SER GLN GLU PRO ARG SER TYR ASN LYS ILE THR VAL VAL GLY VAL GLY ALA VAL GLY MET ALA CYS ALA ILE SER ILE LEU MET LYS ASP LEU ALA ASP GLU VAL ALA LEU VAL ASP VAL MET GLU ASP LYS LEU LYS GLY GLU MET MET ASP LEU GLN HIS GLY SER LEU PHE LEU HIS THR ALA LYS ILE VAL SER GLYLYS ASP TYR SER VAL SER ALA GLY SER LYS LEU VAL VAL ILE THR ALA GLY ALA ARG GLN GLN GLU GLY GLU SER ARG LEU ASN LEU VAL GLN ARG ASN VAL ASN ILE PHE LYS PHE ILE ILE PRO ASN ILE VAL LYS HIS SER PRO ASP CYS ILE ILE LEU VAL VAL SER ASN PRO VAL ASP VAL LEU THR TYR VAL ALA TRP LYS LEU SER GLY LEU PRO MET HIS ARG ILE ILE GLYSER GLY CYS ASN LEU ASP SER ALA ARG PHE

170180190200210220230240250260270280290300311321

5 10

ヌクレオチド結合ドメイン

約130から140アミノ酸で構成

６本のストランドからなるねじれた

オープンβシート

α/β構造

アルコールデヒドロゲナーゼDimethylsulfoxide

NAD+

H3CS

CH3

O

乳酸デヒドロゲナーゼ O

O-H2N

O

Oxamic acid

グリセルアルデヒド-3-リン酸

デヒドロゲナーゼ

O

O-H3C

OPyruvic acid

NAD+

NAD+

イソクエン酸デヒドロゲナーゼ

NADP+

Ca2+（本来は Mn2+）

OO

O-

O-O

O

O-

isocitric acid

β１-αＡ-β２モチーフのアミノ酸配列はよく保存

されている。

アルコールデヒドロゲナーゼ

194-224

NAD+

グリセルアルデヒド-3-リン酸デヒドロゲナーゼ

3-33

乳酸デヒドロゲナーゼ

22-53

NAD+NAD+

グルコースオキシダーゼ

20-50

グルタチオンレダクターゼ

22-50

FAD

FAD