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Государственное бюджетное образовательное учреждение высшего профессионального образования « СМОЛЕНСКАЯ ГОСУДАРСТВЕННАЯ МЕДИЦИНСКАЯ АКАДЕМИЯ » Министерства Здравоохранения Российской Федерации Областное государственное бюджетное учреждение здравоохранения - PowerPoint PPT Presentation
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Государственное бюджетное образовательное учреждение высшего профессионального образования
«СМОЛЕНСКАЯ ГОСУДАРСТВЕННАЯ МЕДИЦИНСКАЯ АКАДЕМИЯ»Министерства Здравоохранения Российской Федерации
Областное государственное бюджетное учреждение здравоохранения«СМОЛЕНСКИЙ ОБЛАСТНОЙ ИНСТИТУТ ПАТОЛОГИИ”
SMOLENSK STATE MEDICAL ACADEMY, Department of Pathological Anatomy
“SMOLENSK REGIONAL INSTITUTE OF PATHOLOGY”
“MORPHOMETRIC PARAMETERS OF MYOCARDIUM AS SUBSTRATE OF MATHEMATICAL MODELING OF MYOCARDIAL
INFARCTION HEALING”
PhD, Assistant of the Department, Clinical PathologistKORNEVA YULIA
»
INTRODUCTION One of the greatest problems
of modern cardiology is chronic heart failure as an outcome of healing of myocardial infarction (MI)
Changes in myocardium, accompanied healing of MI, are known as REMODELING of myocardium
Remodeling is characterized by structural and geometrical changes in heart
Morphologically the process includes hypertrophy of Cardiomyocytes, reorganisation of vessels, fibrosis and loss of Functional elements (especially by means of apoptosis)
Loss of Cardiomyocyt
esFibrosis
Reorganisation of vessels
CHRONIC
HEART FAILURE
Apoptosis of
Cardiomyocytes
Cellular population
Micro-circulation
Cardiomyocytes are known to be cellular populations with very limited potential to regeneration. One of the main effects on the regulation of remodeling have different cellular populations, entering Myocardium through the
capillaries. They also play role in loss of structural elements through apoptosis and formation of new vessels.
MATERIALS OF THE INVESTIGATION Postmortem material - 105 hearts from
patient died due to MI, localized in one of the free walls of left ventricle
• Acute MI 1-2 days
• Acute MY 3-5 days
• Acute MY more then 7 days
Acute MY
Healed MY• Recurrent MI
1-2 days• Recurrent MY
3-5 days• Recurrent MY
more then 7 days
Recurrent MI
Healed MY
MI 1-2 days in duration (H-E, х200)
MI more then days in duration (H-E, х200)
MI 3-5 days in duration (H-E, х200)
Healed MY – scar (H-E, х100)
MATERIALS: The specimens for
investigation were taken from:
The centre of pathological process
The border zone The intact zones
(centre of interventricular
septum an centre of right ventricle)
METHODS: 1. Cellular populations, forming inflammatory
infiltrate, were counted around capillaries; 2. Area of microcirculatory bed was measured
by means of special computer program “Videotest.Morphology. 4.0”;
3. Apoptosis of structural elements was revealed by means of immunohistochemical examination with monoclonal antibodies against Caspase-3;
4. All the data were treated with non-parametrical statistical methods.
Dynamic of changes of cellular populations in the affected zone
during healing of acute and recurrent MI
Dynamic of changes of cellular populations in the border zone
during healing of acute and recurrent MI
Dynamic of changes of cellular populations in the intact zone
(interventricular septum) during healing of acute and recurrent MI
Dynamic of changes of cellular populations in the intact zone
(right ventricle) during healing of acute and recurrent MI
HEALING OF ACUTE MYOCARDIAL INFARCTION Acute MI1-2 days- Acute MI 3-5 days
Acute MI 3-5 days- Acute MI more then 7 days
Acute MI more then 7 days – Healed MI
AZ
BZ
IVSRV
AZ
BZ
IVSRV
AZ
BZ
IVSRV
AZ- affected zone;BZ – border zone;IVS – inter-ventricular septum;RV – right ventricle.
HEALING OF RECURRENT MYOCARDIAL INFARCTION
Recurrent MI1-2 days- Recurrent MI 3-5 days
Recurrent MI 3-5 days- Recurrent MI more then 7 days
Recurrent MI more then 7 days – Healed MI
AZ
BZ
IVSRV
AZ
BZ
IVSRV
AZ
BZ
IVSRV
AZ- affected zone;BZ – border zone;IVS – interventricular septum;RV – right ventricle.
SUBSTRATE FOR MATHEMATICAL MODELING OF VENTRICULAR FIBRILLATION Ventricular fibrillation is one of the fatal and
almost unpredictable complication of myocardial infarction. But the pathogenesis of is still very unclear as well as the criteria for its morphological verification.
We compared cellular infiltrate in myocardium in patients who died due to ventricular fibrillation and the group of cases without such complication, and statistical methods revealed the significant differences in cellular infiltrate.
The finding may be the key for explanation of some steps in pathogenesis of ventricle fibrillation and helped to create a method of objective verification of ventricle fibrillation.
WHAT WE HAVE: 1. Numerical quantity of the changes of cellular infiltrate,
capillary bed and apoptosis of Cardiomyocytes (reflecting process of myocardial remodeling) during healing of myocardial infarction in necrotic zone, border zone and intact zones;
2. Numerical quantities of morphometric parameters of acute and recurrent myocardial infarction healing;
3. Quantitative differences of morphometric parameters of acute and recurrent myocardial infarction healing;
4. Peculiarities of cellular infiltrate in myocardium of patients who died due to ventricular fibrillation as a complication of myocardial infarction.
POSSIBLE PROPOSALS FOR CO-OPERATION:
1. Mathematical model of acute and recurrent myocardial infarction healing, reflecting differences in remodeling of the whole heart during the diseases (based on the changes in cellular infiltrate, microcirculation and death of cardiomyocytes);
2. Mathematical model of ventricular fibrillation as myocardial infarction complication, basing on differences in cellular infiltrate in myocardium of such patients (in some way, it may clarify the dim pathogenesis of the complication);
3. The scheme for prognosis of myocardial remodeling with formation of cardiac insufficiency, based on morphological criteria.
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