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ZOOTAXA
ISSN 1175-5326 (print edition)
ISSN 1175-5334 (online edition)Copyright © 2014 Magnolia Press
Zootaxa 3895 (2): 273–284
www.mapress.com/zootaxa/Article
http://dx.doi.org/10.11646/zootaxa.3895.2.8
http://zoobank.org/urn:lsid:zoobank.org:pub:12702F5B-4E55-4CCA-8664-1F6269EF06EA
Three new species of Eurypon Gray, 1867 from Northeastern Brazil
(Poecilosclerida; Demospongiae; Porifera)
GEORGE GARCIA SANTOS, FERNANDO FRANÇA & ULISSES PINHEIRO1
Universidade Federal de Pernambuco, Centro de Ciências Biológicas, Departamento de Zoologia, Av. Nelson Chaves, s/n Cidade
Universitária CEP 50373-970, Recife, PE, Brazil. 1Corresponding author. E-mail: [email protected]
Abstract
Three new species of Eurypon from Northeastern Brazil are described: Eurypon clavilectuarium sp. nov.; Eurypon suas-
sunai sp. nov. and Eurypon distyli sp. nov. Samples were collected from the continental shelf of Rio Grande do Norte,
Bahia and Paraíba States. While the majority of Atlantic Eurypon species have been described from deep water, two of
three new species described in this study were collected from shallow depths. The three new species from Brazil were
compared with all other eighteen Eurypon species described from the Atlantic.
Key words: Porifera, sponge, Eurypon, new species, Rio Grande do Norte State, Paraíba State, Bahia State, taxonomy,
Brazil
Introduction
Eurypon Gray, 1867 is essentially an encrusting sponge with hymedesmioid or microcionid skeletal arrangement
(Hooper 2002), the difference between the two being related to the relative thickness of species. The most recent
revision of the genus (Aguilar-Camacho & Carballo 2013) describes four new species from the Mexican Pacific
Ocean, essentially summarized as follows. The morphology of echinating acanthostyles of Eurypon is similar to
that found in species belonging to the subgenus Microciona (Genus Clathria; Family Microcionidae). The
hymedesmioid skeleton of Eurypon is a homoplasious character that had been reported in several other genera
(such as: Clathria, Acarnus, Hymedesmia, Timea, Prosuberites and others) (Boury-Esnault et al. 1994; Hooper
1996). Species of Eurypon have tylostyles (or subtylostyles) in one or two categories and acanthostyles as
choanosomal spicules (both of which echinate and are erect on the hymedesmoid basal spongin skeleton), and one
or more categories of oxeas or styles as subectosomal or ectosomal spicules (the former associated with the erect
tylostyles and the latter tangential or forming brushes on the surface). Microscleres if present are raphides (Hooper
2002; Aguilar-Camacho & Carballo 2013). Topsent’s (1894) description mentions dubious chelae and asters (in E.
toureti), but these are indeed foreign as he suspected (Hooper 2002).
The genus currently has 45 valid species with 20 recorded in the Atlantic Ocean, and five of these also
occurring in the Mediterranean Sea (Bertolino et al. 2013; van Soest et al. 2014). So far in Brazil the only records
of the genus are from Amapá State (Collette & Rützler 1977) and Pernambuco State (Muricy & Moraes 1998),
neither of which identified specimens to a species taxon.
In this study, three new species of Eurypon are described from Northeastern Brazil, increasing to 23 the
number of known species in the Atlantic.
Material and methods
Specimens of Eurypon were collected from four localities in Northeastern Brazil (Figure 1). One specimen was
Accepted by J. Hooper: 13 Nov. 2014; published: 15 Dec. 2014 273
collected from one trawl at Rio Grande do Norte State on 2011, as part of the Project ‘Campanha de
Monitoramento Ambiental do Projeto de Caracterização Ambiental do Talude Continental na Bacia Potiguar/ Rio
Grande do Norte/ BR’ (BPot) sponsored by PETROBRAS (Petróleo do Brasil S/A). Other specimens from Paraíba
and Bahia States were collected by snorkeling. Specimens were preserved in 80% ethanol and deposited in the
Porifera collection of Universidade Federal de Pernambuco (UFPEPOR). Dissociated spicule mounts and skeletal
sections were made using classical procedures for Demospongiae (Hajdu et al. 2011). Images of specimens,
sections and SEM (Scanning Electron Microscopy) preparations were obtained digitally. Spicule measurements
from each specimen are presented as minimum–mean–maximum for length/width, with n = 30. Taxonomic
comparisons were made with data tabulated for all species of Eurypon available in the World Porifera Database
(van Soest et al. 2014).
FIGURE 1. Location of the collection sites of Eurypon clavilectuarium sp. nov. (1—Rio Grande do Norte State); Eurypon
distyli sp. nov. (2—Paraíba State); and Eurypon suassunai sp. nov. (3, 4—Bahia State).
Results and discussion
Systematics
Class Demospongiae
Order Poecilosclerida Topsent, 1928
Remarks. From recent molecular evidence it has been proposed to resurrect the concept of Axinellida, although
different in composition from that proposed by Levi (1973) (see Morrow et al. 2012). For the present, until more
fully resolved through better taxón sampling for new molecular evidence, we follow the current systematics usage
of the World Porifera Database (van Soest et al. 2014).
SANTOS ET AL.274 · Zootaxa 3895 (2) © 2014 Magnolia Press
Family Raspailiidae Nardo, 1833
Subfamily Raspailiiniae Nardo, 1833
Genus Eurypon Gray, 1867
Synonymy. For synonymy see Hooper (2002).
Definition. Typically encrusting Raspailiidae with microcionid skeletal structure in which fibre nodes ascend
from the basal layer of spongin (Hooper 2002).
Type species: Hymeraphia clavata Bowerbank, 1866 (by monotypy).
Eurypon clavilectuarium sp. nov.
(Figs 1–3; Table 1)
Type specimens: UFPEPOR 1534 (Holotype), Bacia Potiguar (04º 44.8945' S, 36º 25.4571' W), Rio Grande do
Norte State, Brazil, depth 108 m, trawl, Box 37, col. Petrobras, (23.V.2011).
Diagnosis. Eurypon clavilectuarium sp. nov. is the only Eurypon described from the Atlantic with large
choanosomal subtylostyles (1200–2000 µm long), and raphidiform styles (440 µm long).
External morphology (Fig. 2A–B). Encrusting sponge (in calcareous nodules), up to 1 mm thick. Oscules not
visible. Surface hispid due to evenly distributed projecting spicules. Consistency is fragile. Color in life is
unknown and brownish-purple in ethanol. However, the specimens were collected in the same drag as Aiolochroia
crassa (Hyatt, 1875) and stored in the same container. It is possible that the A. crassa pigments stained the Eurypon
specimens (as also reported for a specimen of Aulospongus by Cavalcanti et al. 2014).
Skeleton (Fig. 2C). Skeletal structure is hymedesmioid composed by long subtylostyles, two categories of
echinating acanthostyles and raphidiform styles with their bases embedded in basal spongin and points protruding a
long way through the surface, forming a dense erect palisade on the basal spongin.
FIGURE 2. Eurypon clavilectuarium sp. nov. (UFPEPOR 1534, holotype). A, preserved specimen; B, details of the surface; C, skeleton. Scale bars: A = 1 cm; B = 1.5 mm; C = 250 µm.
Spicules (Fig. 3A–G). Choanosomal subtylostyles (Fig. 3A, E): long, smooth, slender, slightly curved and
with bulbous to styloid base (1200–2000 / 8–24 µm); Acanthostyles I (Fig. 3B, F): varying from straight to
slightly curved, with lightly bulbous base, short and curved spines (hook-shaped spines) more concentrated in the
Zootaxa 3895 (2) © 2014 Magnolia Press · 275NEW EURYPON FROM BRAZIL
middle and apical part of spicules. Spination along the shaft is very sparse (195–600 / 7–17 µm). Acanthostyles II
(Fig. 3C, G): straight, fusiform, base frequently styloid, totally microspined (spines are always recurved and
arranged the same as previous category) (72–114 µm); Raphidiform styles (Fig. 3D): long, smooth, with styloid
base, slender, ranging from straight to curved, some sinuous (260–439 / 1.9–3 µm).
FIGURE 3. Spicule composition of Eurypon clavilectuarium sp. nov. (UFPEPOR 1534, holotype) in SEM. A, subtylostyle; B, acanthostyle I; C, acanthostyle II; D, raphidiform style; E, details of ends of A; F, details of ends of B; G, details of ends of C. Scale bars: A = 150 µm; B = 60 µm; C–D = 30 µm; E–G = 10 µm.
Distribution (Fig. 1). Northeastern coast of Brazil, Rio Grande do Norte State, Brazil.
Depth. 108 m.
Etymology. The species is named clavilectuarium (clavus = nail, lectuarius = of a bed), due to the long
spicules perpendicular and projecting from the basal spongin on the substate, resembling a bed of nails.
Remarks. The new species belongs to the genus by the presence of choanosomal subtylostyles, echinating
acanthostyles, subectosomal raphidiform styles, and an encrusting habit. Eurypon clavilectuarium sp. nov. is the
unique species of the genus from Atlantic which has large choanosomal subtylostyles (1200–2000 µm) and
raphidiform styles (439 µm). The presence of two categories of acanthostyles in E. clavilectuarium sp. nov. differs
the following species from Atlantic: E. cinctum Sarà, 1960; E. clavatella Little, 1963; E. coronula (Bowerbank,
1874); E. fulvum Lévi, 1969; E. lacazei (Topsent, 1891); E. lictor (Topsent, 1904); E. major Sarà & Siribelli, 1960;
SANTOS ET AL.276 · Zootaxa 3895 (2) © 2014 Magnolia Press
E. obtusum Vacelet, 1969; E. radiatum (Bowerbank, 1866); E. toureti (Topsent, 1894); E. viride (Topsent, 1889).
Except for E. radiatum, all of these species present tylostyles which are not found in E. clavilectuarium sp. nov. In
addition, the following species differ by the presence of oxeas (E. denisae; E. cinctum; E. fulvum; E. major; E.
obtusum); tornotes (E. lacazei; E. lictor; E. mucronale); isochelae (E. toureti); and raphides (E. viride) (see
character list in Table 1).
Eurypon suassunai sp. nov.
(Figs 1, 4–5; Table 1)
Type specimens: UFPEPOR 1532 (Holotype), off Canavieiras City, Camamu-Almada Basin (15° 35' 5.91" S, 38°
46' 36.35" W), Bahia State, Brazil, (X.2011). UFPEPOR 1533 (paratype), off Canavieiras City, Camamu-Almada
Basin (15° 33' 30.56" S, 38° 45' 14.53" W), Bahia State, Brazil, (X.2011).
Diagnosis. Eurypon suassunai sp. nov. is the only Eurypon from the Atlantic which combines subtylostyles,
acanthostyles I, acanthostyles II with bulbous base, and raphidiform styles.
External morphology (Fig 4A–B). Thin transparent encrustation, about 1 mm thick. Oscules not visible.
Surface hispid due to evenly distributed projecting spicules. Consistency is fragile. Color in life is orange
(formaldehyde) and pale in ethanol.
Skeleton (Fig. 4C–D). Ectosomal skeleton is absent. Tips of the choanosomal spicules protrude externally
(Fig. 4C–D). The choanosomal skeleton has a hymedesmioid structure. The raphidiform styles are dispersed in the
subectosomal region. Acanthostyles I and II are most abundant on basal layer of spongin (Fig. 2D).
FIGURE 4. Eurypon suassunai sp. nov. (UFPEPOR 1532, holotype). A, arrows indicate the position of the specimen; B, preserved specimen and details of the surface; C, skeleton; D, details of the choanosomal skeleton (hymedesmioid). Scale bars: A = 5 mm; B = 1.5 mm; C = 200 µm; D = 100 µm.
Spicules (Fig. 5A–I). Choanosomal subtylostyles (Fig. 5A, G): long, smooth, slender, slightly curved and
with bulbous base (690–1660 / 5–17 µm); Acanthostyles I (Fig. 5B, E): varying from straight to slightly curved,
with lightly bulbous base, short and curved spines (hook-shaped spines) more concentrated in the middle and apical
part of spicules (159–354 / 7–13 µm); Acanthostyles II (Fig. 5C, F): are short and robust, often straight, with
bulbous base, totally microspined with short and curved spines (hook-shaped spines) (54–129 µm); Raphidiform
styles (Fig. 5D, H, I): smooth, thin, most are straight, the styloid base ranges from the shape of a crown (171–345
/ 1–5 µm).
Distribution (Fig. 1). Northeastern coast of Brazil, Bahia State, Brazil.
Depth. Shallow water.
Etymology. This species is named in honor of the Late the famous writer Ariano Vilar Suassuna for his
defense of the culture of the Brazilian Northeast.
Zootaxa 3895 (2) © 2014 Magnolia Press · 277NEW EURYPON FROM BRAZIL
TA
BL
E 1
. Com
para
tive
mic
rom
etric
dat
a on
the
spic
ules
and
ove
rvie
w o
f dis
tribu
tion
of th
e liv
ing
spec
ies
of E
uryp
on G
ray,
186
7 fo
r the
Atla
ntic
. Val
ues
are
in m
icro
met
ers
(µm
), ex
pres
sed
as m
inim
um–m
axim
um o
r min
imum
–mean–
max
imum
leng
th/w
idth
. Ref
eren
ces a
re n
umbe
red
in p
aren
thes
es a
nd li
sted
at t
he fo
ot o
f the
tabl
e.�
Spec
ies
Spec
imen
lo
catio
n D
epth
(m)
Spic
ules
(µm
)
A
cant
host
yles
Su
bect
osom
al
Styl
es
Cho
anos
omal
T
ylos
tyle
s C
hoan
osom
al
Subt
ylos
tyle
s O
ther
spic
ules
Eury
pon
clav
ilect
uari
um sp
. nov
. R
io G
rand
e do
N
orte
Sta
te,
Bra
zil
108
I) 1
95–329
–600
/ 7–10
–17
II) 7
2–87
–114
260–354.5–
439
/ 1.
9–2.7–
3.1
(rap
hidi
form
)
- 12
00–1659–
2000
/ 8–17
–24
-
Eury
pon
suas
suna
i sp.
nov
. B
ahia
Sta
te,
Bra
zil
Shal
low
wat
erI)
159
–243
–354
/ 7–10
–13
II) 5
4–77
–129
171–266–
345
/ 1–
5 (r
aphi
difo
rm)
- 69
0–1147
–166
0 /
5–11
–17
-
Eury
pon
disty
lisp
. nov
.Pa
raíb
a St
ate,
B
razi
l 1
67–89.8–
129
/ 6–7.1–
8I)
315
–398.8
–485
/ 5
–6.9
–7
II) 3
15– 371.3–
437
/ 3– 4.3–
4.5
(o
r ani
soxe
as)
- 10
60–1701.6–
2810
/ 7
–10.7–
18
122–283.9–
373
(raph
ide)
E. d
enis
ae V
acel
et, 1
969
(10)
Med
iterr
anea
n Se
a 30
0–35
0 I)
120
–150
/ 7–
10II
) 50–
90 /
7–8
- 31
50 /
29
- 25
0–30
0 / 4
.5–7
.5 (o
xea)
E. c
inct
um S
arà,
196
0 (1
0)N
aple
s 70
31
–316
/ 7–
16
- 25
00 /
8–30
415–
510
/ 5–9
(sty
le/o
xea)
E.
cla
vate
lla L
ittle
, 196
3 (1
)G
ulf o
f Mex
ico
10
75–102
–145
/ 5–
6–9
351–361–
392
/ 4
249–384–
470
/ 14
–15–
21
- -
E. c
lava
tum
(Bow
erba
nk, 1
866)
(12)
Shet
land
s, R
osco
ff, Il
es d
e G
léna
n an
d A
zore
s
40–1
300
65–6
00
500
/ 3
1500
/ 18
-
-
E. c
oron
ula
(Bow
erba
nk, 1
874)
(10)
Shet
land
Isla
nds
and
Nor
way
60
25
4 -
635–
1411
-
-
E. fu
lvum
Lév
i, 19
69 (2
)V
ema
Seam
ount
30
–180
75
–85
/ 9
- 11
00–1
500
/ 12
- 4
75–5
30 /
7–8
(oxe
a)
E. h
ispid
ulum
(Top
sent
, 190
4) (3
)A
zore
s 59
9–88
0 I)
500
/ 17
II
) 160
–200
/ 10
–12
- -
325–
365
/ 4
(mic
rosp
ined
) -
E. la
caze
i (To
psen
t, 18
91) (
4)R
osco
ff 40
–600
75
–80
/ 10
- 20
00 /
20
- 23
0 / 7
(tor
note
)
......
cont
inue
d on
the
next
pag
e
SANTOS ET AL.278 · Zootaxa 3895 (2) © 2014 Magnolia Press
TA
BL
E 1
. (co
ntin
ued)
Spec
ies
Spec
imen
lo
catio
n D
epth
(m)
Spic
ules
(µm
)
A
cant
host
yles
Su
bect
osom
al
Styl
es
Cho
anos
omal
T
ylos
tyle
s C
hoan
osom
al
Subt
ylos
tyle
s O
ther
spic
ules
E. li
ctor
(Top
sent
, 190
4) (3
)A
zore
s 16
00
- -
2000
/ 23
-
575
–100
0 / 4
–5 (t
orno
te)
50
/ 13–
15
(tric
hodr
agm
ata)
E.
mix
tum
(Top
sent
, 192
8) (5
) C
euta
, Spa
nish
M
oroc
co
650–
2165
I)
100
0
II) 1
00–3
20
- N
ot re
cord
ed
Not
reco
rded
-
E. m
ajor
Sar
à &
Siri
belli
, 196
0 (1
0)
Nap
les a
nd
Wes
tern
Eur
ope
14–4
0 80
–220
/ 4–
10.5
-
1115
–221
0 /
10–1
7 -
480–
700
/ 4–7
.5 (o
xea)
E. m
ucro
nale
(Top
sent
, 192
8) (5
) A
zore
s 24
60
I) 7
00–9
00 /
25–
30II
) 125
–280
/ 20
- -
Not
reco
rded
40
0–49
0 / 1
2–17
(tor
note
)
E. o
btus
um V
acel
et, 1
969
(10)
Med
iterr
anea
n Se
a 25
0 70
–170
/ 5–
7.5
- N
ot re
cord
ed /
10–1
2 -
400–
430
/ 2.5
–3 (o
xea)
E. p
ilose
lla (T
opse
nt, 1
904)
(3)
Azo
res
550–
1360
I)
200
0–30
00 /
25
–30
II
) 170
–300
/ 15
–25
- -
300–
480
/ 5–6
(m
icro
spin
ed)
-
E. ra
diat
um (B
ower
bank
, 186
6) (1
1)
Azo
res,
Shet
land
s an
d H
ebrid
es
Not
reco
rded
100–
400
I) 8
00 /
10–1
5 II
) 350
-
- -
E. sc
abio
sum
(Top
sent
, 192
7) (6
) A
zore
s 65
0–91
9 I)
100
0 / 2
4 II
) 80–
300
/ 6–1
3-
- 47
0–63
0 /3
–4
-
E. si
mpl
ex (B
ower
bank
, 187
4) (7
) R
osco
ff an
d Fa
roes
60
–260
I)
218
/ 8
II) 1
05
- -
2116
/ 27
-
E. to
psen
ti (B
urto
n, 1
954)
(13)
Wes
tern
C
arib
bean
90
0 -
1200
/ 8
1600
/ 14
-
60 (t
richo
drag
mat
a)
E. to
uret
i (To
psen
t, 18
94) (
8)
Gul
f of M
exic
o N
ot re
cord
ed50
–60
- N
ot re
cord
ed
- 10
–12
(isoc
hela
e)
E. v
irid
e (T
opse
nt, 1
889)
(9)
Gul
f of M
exic
o,
Med
iterr
anea
n an
d A
zore
s
80–8
00
85
- 10
00
- 70
(rap
hide
)
Ref
eren
ces:
(1) L
ittle
(196
3); (
2) L
évi (
1969
); (3
) Top
sent
(190
4); (
4) T
opse
nt (1
891)
; (5)
Top
sent
(192
8); (
6) T
opse
nt (1
927)
; (7)
Bow
erba
nk (1
874)
; (8)
Top
sent
(189
4); (
9) T
opse
nt (1
889)
; (10
) A
guila
r-C
amac
ho &
Car
ballo
(201
3); (
11) B
ower
bank
(186
6); (
12) A
rndt
(193
5); (
13) B
urto
n (1
954)
.
Zootaxa 3895 (2) © 2014 Magnolia Press · 279NEW EURYPON FROM BRAZIL
FIGURE 5. Spicule composition of Eurypon suassunai sp. nov. (UFPEPOR 1532, holotype) in SEM. A, subtylostyle; B, acanthostyle I; C, acanthostyle II; D, raphidiform style; E, details of ends of B; F, details of ends of C; G, details of the base of A; H and I, details of the base of D. Scale bars: A = 200 µm; B = 80 µm; C = 30 µm; D = 80 µm; E–F = 5 µm; G = 10 µm; H–I = 10 µm.
Remarks. Eurypon suassunai sp. nov. belongs to the genus in having choanosomal subtylostyles, echinating
acanthostyles and an encrusting habit with a hymedesmioid skeleton. The closest species to E. suassunai sp. nov. is
E. clavilectuarium sp. nov. because both shares the same spicules. However, in E. clavilectuarium sp. nov. the
subtylostyles are longer and stouter (1200–2000 / 8–24) than those of E. suassunai sp. nov. (690–1600 / 5–17) and
in the former, the tyle of the acanthostyles II are styloid against bulbose tyle of the last. Except from both, no others
species of Eurypon in Atlantic have raphidiform styles. Furthermore, the presence of two categories of
acanthostyles distinguishes E. suassunai sp. nov. from eleven species of the Atlantic: E. cinctum; E. clavatella; E.
coronula; E. fulvum; E. lacazei; E. lictor; E. major; E. obtusum; E. radiatum; E. toureti; E. viride (Table 1).
Eurypon distyli sp. nov.
(Figs 1, 6–7; Table 1)
Type specimens. UFPEPOR 1601 (Holotype), Ponta do Seixas (07º 09' 16" S, 34º 47' 35" W), João Pessoa,
Paraíba State, Brazil, depth 1 m, col. G.G. Santos, (05.XI.2013). Paratype. UFPBPOR 1620 (collected together with
the holotype).
SANTOS ET AL.280 · Zootaxa 3895 (2) © 2014 Magnolia Press
Diagnosis. Eurypon distyli sp. nov. is the only Eurypon in the Atlantic with two categories of styles and large
raphides.
External morphology (Fig. 6). Encrusting sponge, covering surfaces up to 2 cm² diameter and 0.5–1.5 mm
thick, growing over rocks (Fig. 6A). Oscules not visible. Surface hispid due to evenly distributed projecting
spicules and with some bouquets of spicules irregularly distributed (Fig. 6B). Consistency firm and difficult to tear.
Color in life is green, dark green in ethanol.
FIGURE 6. Eurypon distyli sp. nov. (UFPEPOR 1601, holotype). A, fresh holotype taken out of the water, showing spicules; B, details of the surface; C, basal layer of spongin with echinating acanthostyles. Scale bars: A = 1 cm; B = 200 µm; C = 100 µm.
Skeleton (Fig. 6). The ectosomal skeleton is absent, with tips of choanosomal the spicules externally
protruding (Fig. 6B). The choanosomal skeleton is a hymedesmioid structure with a basal layer of acanthostyles,
auxiliary styles and principal subtylostyles, are all perpendicular to the substrate (Fig. 6B and C). The
subectosomal styles I and II are dispersed in the subectosomal region of the erect spicule skeleton.
Spicules (Fig. 7A–I). Choanosomal subtylostyles (Fig. 7A, G): long, smooth, slender, slightly curved and
with bulbous base (1060–2810 / 7–18 µm); Subectosomal styles I (Fig. 7B, H): smooth, lightly robust and often
straight (315–485 / 5–7 µm); Subectosomal style II (anisoxea) (Fig. 7C, I): smooth, thin and varying from
straight to curved (315–437 / 3–4.5 µm); Raphides (Fig. 7D): usually straight, smooth and with sharp ends
(121–372 / less than 0.5 µm); Acanthostyles (Fig. 7E–F): usually straight, fusiform, with base slightly bulbous
and surface completely spined. The spines are short and curved (67–129 µm long), at the base the spines are hook-
shaped and in the middle region the spines are spatulate (see Fig. 7F).
Distribution (Fig. 1). Northeastern coast of Brazil, Paraíba State, Brazil.
Depth. 1 m.
Etymology. The name refers to the two size categories of subectosomal styles.
Remarks. The closest species to Eurypon distyli sp. nov. is E. radiatum from the Azores because both share
the posession of two categories of subectosomal styles and one category of acanthostyle, although E. radiatum
lacks choanosomal subtylostyles and raphides. Aside from these two species no other Eurypon described from the
Atlantic have two categories of subectosomal styles. Other species from Atlantic that resemble Eurypon distyli sp.
nov. is E. viride, both having raphides. However, E. viride has small raphides (70 µm long), tylostyles and a white
color compared to large raphides (280 µm long), subtylostyles and a green color in E. distyli sp. nov. (Table 1).
Zootaxa 3895 (2) © 2014 Magnolia Press · 281NEW EURYPON FROM BRAZIL
FIGURE 7. Spicule composition of Eurypon distyli sp. nov. (UFPEPOR 1601, holotype). A, subtylostyle; B, style I; C, style II; D, raphide; E, acanthostyle; F, details of the flattened spines; G, details of the base of A; H, details of the base of B; I, details of the base of C. Scale bars: A = 200 µm; B = 40 µm; C = 50 µm; D = 30 µm; E, G, H = 10 µm; F = 5 µm; I = 3 µm.
Discussion
The three new species described here display the typical morphology of Eurypon: the presence of choanosomal
styles or (sub-)tylostyles, echinating acanthostyles, subectosomal or ectosomal spicules (styles, oxeas and
raphides), and an encrusting habit with a hymedesmioid or microcionid basal spongin skeleton (Hooper 2002), the
latter depending on how thick the specimens are. We compare the three new species with congeners from the
Atlantic, and conclude that they are sufficiently different from our three new species, and therefore are not
considered further here.
In Raspailiidae, Eurypon is distinguished from Raspailia Nardo, 1833 in having only up to three categories of
megascleres whereas typical Raspailia have four categories (Hooper 1991, 2002). According Hooper (2002) this is
an attempt to separate species based on their skeletal architecture.
SANTOS ET AL.282 · Zootaxa 3895 (2) © 2014 Magnolia Press
Many Eurypon species from the Atlantic have so far been described from deep water (40–2165 m), and Picton
(1990) proposed this genus was indicative of deep water sponge communities. Although, exceptions have been
recorded. Little (1963) described E. clavatella from Gulf of Mexico from 10 m depth, and Sarà & Siribelli (1960)
recorded E. major from Western Europe from 14–40 m (van Soest et al. 2000), as well as two species described
here from shallow water habitats.
With the description of Eurypon clavilectuarium sp. nov., Eurypon suassunai sp. nov. and Eurypon distyli sp.
nov., there are now 23 species of the genus in the Atlantic Ocean, and 48 valid species worldwide.
Acknowledgements
The authors are grateful to PETROBRAS for donating the holotype (UFPEPOR 1534) specimen and providing
collection data. George G.S. thanks CAPES for providing a doctoral scholarship. Authors are also thankful to
Mauricio Paiva, Janaina Melo, Dyego Oliveira, Gaby Vasconcelos and Josineide Correia for SEM facilities at
CETENE (Centro de Tecnologias Estratégicas do Nordeste), Dr. André Esteves (UFPE), Dr. Jesser Fidelis (UFPE),
Dr. Carlos Pérez (UFPE), Thaynã Cavalcante, M.Sc. Lucas Lima (UESPI), Rudá Lucena, Diego Aquino, M.Sc.
Luíz Aquino, Yuri Niella, and Adélia Alliz for technical support. We further thank CNPq (Edital PROTAX 2010 -
562320/2010-5), and FACEPE, for providing grants and/or fellowships, and to two anonymous reviewers for
improving the manuscript.
References
Aguilar-Camacho, J.M. & Carballo, J.L. (2013) Raspailiidae (Porifera: Demospongiae: Axinellida) from the Mexican Pacific Ocean with the description of seven new species. Journal of Natural History, 47, 25–28, 1663–1706. http://dx.doi.org/10.1080/00222933.2013.769642
Arndt, W. (1935) Porifera. Die Tierwelt der Nord- und Ostse, 3a (27), 1–140.http://dx.doi.org/10.3897/zookeys.336.5139
Bertolino, M., Cerrano, C., Bavestrello, G., Carella, M., Pansini, M. & Calcinai, B. (2013) Diversity of Porifera in the Mediterranean coralligenous accretions, with description of a new species. ZooKeys, 336, 1–37.
Bowerbank, J.S. (1866) A monograph of the British Spongiadae. II. Synopsis of Genera. Ray Society of London, London, 388 pp.
Burton, M. (1954) Sponges. In: The 'Rosaura' Expedition. Part 5. Bulletin of the British Museum (Natural History) Zoology, 2 (6), pp. 215–239.
Cavalcanti, T., Santos, G.G. & Pinheiro, U. (2014) Two new species of Aulospongus Norman, 1878 with a key to the Atlantic species (Poecilosclerida; Demospongiae; Porifera). Zootaxa, 3827 (2), 282–292. http://dx.doi.org/10.11646/zootaxa.3827.2.9
Collette, B.B. & Rützler, K. (1977) Reef fishes over sponge bottoms off the mouth of the Amazon River. In: Proceedings of the
3rd International Coral Reef Symposium. University of Miami, Miami, pp. 305–310.Hajdu, E., Peixinho, S. & Fernandez, J. (2011) Esponjas marinhas da Bahia: Guia de campo e laboratório. Museu Nacional,
Rio de Janeiro, 276 pp. [Série Livros 45] Hartman, W.D. (1982) Porifera. In: Parker, S.P. (Ed.), Synopsis and Classification of Living Organisms. Vol. 1. McGraw-Hill
Book Co., New York, pp. 641–663.Hooper, J.N.A. (1991) Revision of the family Raspailiidae (Porifera: Demospongiae), with description of Australian species.
Invertebrate Taxonomy, 5 (6), 1179–1418. http://dx.doi.org/10.1071/it9911179
Hooper, J.N.A. (1996) Revision of Microcionidae (Porifera: Poecilosclerida: Demospongiae), with description of Australian species. Memoirs of the Queensland Museum, 40, 1–626.
Hooper, J.N.A. (2002) Family Raspailiidae Hentschel, 1923. In: Hooper, J.N.A. & van Soest R.W.M. (Eds.), Systema Porifera:
a guide to the classification of sponges. Kluwer Academic/Plenum Publishers, New York, pp. 469–510.Lévi, C. (1969) Spongiaires du Vema Seamount (Atlantique Sud). Bulletin du Muséum national d’Histoire naturelle, Series 2,
41 (4), 952–973.Little, E.J. (1963) The Sponge fauna of the St. George’s Sound, Apalachee Bay, and Panama City regions of the Florida Gulf
Coast. Tulane Studies in Zoology, 11, 31–71.Morrow, C.C., Picton, B.E., Erpenbeck, D., Boury-Esnault, N., Maggs, C.A. & Allcock, A.L. (2012) Congruence between
nuclear and mitochondrial genes in Demospongiae: a new hypothesis for relationships within the G4 clade (Porifera: Demospongiae). Molecular Phylogenetics and Evolution, 62, 174–190.
Zootaxa 3895 (2) © 2014 Magnolia Press · 283NEW EURYPON FROM BRAZIL
http://dx.doi.org/10.1016/j.ympev.2011.09.016Muricy, G. & Moraes, F.C. (1998) Marine sponges of Pernambuco State, NE Brazil. Revista Brasileira de Oceanografia, 46 (2),
213–217. http://dx.doi.org/10.1590/s1413-77391998000200009
Picton, B.E. (1990) The sessile fauna of sublittoral cliffs. In: Myers, A.A. et al. (Eds.), The ecology of Lough Hyne. Proceedings of a Conference, Cork, Ireland, 4–5 September 1990, pp. 139–142. [Royal Irish Academy, Dublin]
Sarà, M. & Siribelli, L. (1960) La fauna di Poriferi delle ‘secche’ del Golfo di Napoli. 1. La ‘secca’ della Gaiola. Annuario
dell’Istituto e Museo de Zoologia dell’Università di Napoli, 12 (3), 1–93.Topsent, E. (1889) Quelques spongiaires du Banc de Campêche et de la Pointe-à-Pître. Mémoires de la Société Zoologique de
France, 2, 30–52.Topsent, E. (1891) Deuxième contribution a l’étude des Clionides. Archives de Zoologie Expérimental et Générale, 9, 555–592.Topsent, E. (1894) Étude monographique des spongiaires de France I. Tetractinellida. Archives de Zoologie Expérimentale et
Générale, 3, 259–400, pls. XI–XVI.Topsent, E. (1904) Spongiaires des Açores. Résultats des campagnes scientifiques accomplies par le Prince Albert I. Monaco,
25, 1–280, pls. 1–18. http://dx.doi.org/10.5962/bhl.title.61852
Topsent, E. (1927) Diagnoses d'Éponges nouvelles recueillies par le Prince Albert ler de Monaco. Bulletin de l'Institut
océanographique Monaco, 502, 1–19.Topsent, E. (1928) Spongiaires de l’Atlantique et de la Méditerranée provenant des croisières du Prince Albert Ier de Monaco.
Résultats des campagnes scientifiques accomplies par le Prince Albert I. Monaco, 74, 1–376, pls. I–XI. http://dx.doi.org/10.5962/bhl.title.2169
Van Soest, R.W.M., Picton, B., & Morrow, C.C. (2000) Sponges of the North East Atlantic. Amsterdam: ETI, Springer-Verlag. ISBN 3-540-14774-8 (Windows CD-ROM). Available from: http://species-identification.org/species.php?species_group=sponges&menuentry=inleiding (accessed 5 November 2014)
Van Soest, R.W.M., Boury-Esnault, N., Hooper, J.N.A., Rützler, K., de Voogd, N.J., Alvarez, B., Hajdu, E., Pisera, A.B., Vacelet, J., Manconi, R., Schoenberg, C., Janussen, D., Tabachnick, K.R., Klautau, M. & Picton, B. (2014) World Porifera database. Available from: http://www.marinespecies.org/porifera (accessed 1 March 2014)
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