Private alleles distributions for the conservation of Sumatran orangutans (Pongo abelii)

PUJI RIANTI, DYAH PERWITASARI-FARAJALLAH, DONDIN SAJUTHI,

ALEX NATER, MICHAEL KRÜTZEN

© Rianti 2011

Private alleles distributions for the conservation of

Sumatran orangutans (Pongo abelii)

1: 1000000

U

2 subspecies

12-Sep-15 2© Rianti 2011

Sumatran orangutans

(Rianti. Dissertation. 2015)

1: 1000000

U

1: 1000000

U

WAR

NAR

EAR

BT

12-Sep-15 3ICOBIO_2015

(Rianti et al. Intl. J. Primatol. in reviews)

STRUCTURE (autosomal loci)

F: Highest

F: Lowest

Sumatran orangutans

1: 1000000

U

1: 1000000

U


Migration patterns (autosomal loci)

Demographic History

Sumatran orangutans

(Nater et al. 2015; Rianti et al. Intl. J. Primatol. in reviews)

(Reynolds et al. 2015)12-Sep-15 5

Question ?

Species conservation management

Preserved genetic variation:

Natural selection

& other processes Respon environmental changes

Level of genetic variations

Information in the genetic management:

endangered populations for long-term resilience


DNA Samples :

150 individuals

28 autosomal loci:

- 15 human derived

- 13 Pongo specific

Analysis:

- HP-Rare v1.1

- GenAlEx v4.3

- MetaPop v2.0.a1

Sumatran orangutans

No Locations Locus Allele Frequency n No Locations Locus Allele Frequency n

1 Batang Toru Chr5 135 0.292

11

20 Ketambe O4_6 105 0.024

2 Batang Toru Chr7 183 0.5 21 Ketambe O4C9 205 0.025

3 Batang Toru Chr7 187 0.045 22 Langkat D13S765 183 0.0292

4 Batang Toru D13S321 185 0.231 23 Langkat D16S420 179 0.176

5 Batang Toru D1S550 130 0.346 24 North Aceh D2S141 138 0.067 1

6 Batang Toru D2S1326 224 0.154 25 Pakpak Bharat O4B17 158 0.1672

7 Batang Toru D3S2459 197 0.125 26 Pakpak Bharat O4B24 165 0.1

8 Batang Toru D6S501 162 0.462 27 Suaq Balimbing Chr5 175 0.036

10

9 Batang Toru O4A5 196 0.038 28 Suaq Balimbing Chr7 195 0.027

10 Batang Toru O4B3 307 0.154 29 Suaq Balimbing Chr7 235 0.068

11 Batang Toru O4C9 179 0.154 30 Suaq Balimbing D13S321 197 0.01

12 Batu Ardan O4B3 279 0.071 1 31 Suaq Balimbing D13S321 225 0.01

13 Blangkejeren O4B6 280 0.056 1 32 Suaq Balimbing D16S420 177 0.02

14 Ketambe Chr7 147 0.025

8

33 Suaq Balimbing D1S550 172 0.196

15 Ketambe Chr7 171 0.025 34 Suaq Balimbing D5S1470 226 0.01

16 Ketambe D16S420 193 0.026 35 Suaq Balimbing O4B17 152 0.01

17 Ketambe D2S1326 204 0.024 36 Suaq Balimbing O4C9 213 0.011

18 Ketambe D5S1470 176 0.026 37 Tripa O4B6 265 0.056 1

19 Ketambe D5S1470 180 0.026

Private alleles in each locus per geographical distribution


37 Private Alleles in 18 Loci “DNA Fingerprinting”

Alleles Distributions



Average allele/locus: 9.39 (from 5 to 22 alleles within 28 loci)

1: 500000

U


Allelic Diversity

BL: 0.178

(2.153)

BL: 0.132

(-0.578)

VS

Genetic Diversity

BL: 0.027

BL: 0.003 (1.515)

(-0.662)

If Batang Toru

population GONE

highest loss in

gene & allele pool

Coancestry


(Reynolds et al. 2015)12-Sep-15 10

Implication to Conservation

Batang Toru

the ONLY pop of Sumatran orangutan subspecies

(south area of Lake Toba) : CONSERVATION PRIORITIES

Allelic database Coancestry/Paternity test

Alleles retention; isolated geographic distribution

- population growth

- abundance

- life history characteristics

12-Sep-15 11

Implication to Conservation

Private alleles; isolated small pop

↑ unique biological diversity characteristics

Private alleles specific geographic distribution/population

Conservation programs

Identify the population of origin

Population recovery strategies

(conservation plans of Sumatran orangutan species)

ACKNOWLEDGMENTS

12-Sep-15 12ICOBIO_2015

Thank You

Documents

Private alleles distributions for the conservation of Sumatran orangutans (Pongo abelii)