SEMINAR

ANKUSH MEENA

B100974CH

DEPATMENT OF CHEMICAL ENGINEERING

NATIONAL INSTITUTE OF TECHNOLOGY CALICUT

12/03/2014

PRODUCT RECOVERY

The Product recovery operation are also known as downstream processing steps.

Product recovery in bioprocessing plays a vital role.

Introduction

Downstream- ‘After the fermentation process’.

Primary ‘unit operations’ of Downstream Processing

Cell recovery /removal Dewatering

What is downstream Processing

Secondary ‘unit operations’ Protein purification

Adsorption chromatography Protein processing

Immobilisation Prilling

Protein packaging Sterilisation etc

Downstream processing

The cell disruption techniques may be classified as• Physical method • Chemical method

The following unit operations are used for the separation of solids : • Filtration • Sedimentation • Centrifugation • Foam Separation• Precipitation

System Type With phase change

Without phase change

Solid-liquid Soluble Drying, Evaporation,Adsorption

Ultrafiltration, Reverse osmosis

Insoluble Drying Filtration, Sedimentation ,

Liquid-liquid Soluble Distillation Chromatography

Insoluble Extraction Centrifugation

Solid-solid immiscible Selective Solubilisation

Air Separation ScreeningsievingMiscible Cell disruption

Liquid-liquid-solid

Miscible Immiscible

Adsorption Centrifugation Sedimentation Decanting

Various unit operation in bioprocessing for separation of different phase

It is based on the differences in the surface activity.

It is chemical process of removing a component of a liquid mixture using a bubbled gas and a surfactant to collect the material.

Wastewater treatment systems often use foam separation steps to remove solids from the water stream.

Foam Separation

Foam separation diagram

It is also a very useful method for product recovery by adding the precipitation agent .

The compounds from the corresponding salts or the suspending solids which would settle down to the bottom.

This method is extensively used for product recovery of protein isolates or protein concentrates from oil seeds

Precipitation

Filtration is a physical or mechanical process to separate solids particle from fluids.

Filtration is a technique used for two main purposes.

The first is to remove solid impurities from a liquid.

The second is to collect a desired solid from the solution.

It is a very effective and convenient method of separating the suspending solids from the slurries .

The fluid that pass through is called a filtrate.

Filtration

Filtration is used to separate particles and fluid in a suspension, where the fluid can be a liquid, a gas or a supercritical fluid.

Filtration, as a physical operation is very important in chemistry for the separation of materials of different chemical composition.

Filtration is also important and widely used as one of the unit operations of chemical engineering.

Application of filtration

pressure drop Viscosity of filtrate Area of filtering surfaceResistance of filtrate cake Resistance of filtrate surface Properties of slurry

Factor affected on filtration

Filters are divided into three main categories 1. Cake filters2. Clarifying filters3. Cross-flow filters

Slurries with high solid concentration are operated in cake filters.

Very dilute liquids are passed through the clarifying filters.

The cross-flow filters are used for concentrating the solutions by using filtration technique.

Cross-flow filters

Thank you