HIV-1 Antibodies

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CD4 gp120trimer

Helper T-cell

MembraneCCR40co-receptor

HIV-1 Membrane

The majority of antibodies that target gp120 don’t neutralize HIV

CD4 Binding

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Glycosylation

CD4 Binding

Glycosylation

Variable Loops

CD4 Binding

Heavychain

Light chainLight chain

Heavychain

Antibodies are multi-domain proteins secreted by B cells

antigenbinding sites

Antibody-encoding genes get randomly rearranged in B cells to increase diversity

Germ Line DNASame

for all cells

Variable Constant

Germ Line DNASame

for all cells

Variable Constant

Germ Line DNASame

for all cells

Rearranged DNAUnique to each B cell (allele)

Variable Constant

Germ Line DNASame

for all cells

mRNA2,644,240 potential

combinations

Rearranged DNAUnique to each B cell (allele)

B cells undergo fast naturalselection, which further increases diversity

B cellsproliferate with

high mutation rate

B cellsproliferate with

high mutation rate

Follicular Dendritic Cells

select forbinding affinity

Effective antibodies differ on sequence but share similar structures

VLightVHeavy

Same germ line allele

VLightVHeavy

Similar binding suggests convergent evolution

Paratope

SharedEpitope

Germ Line

Mature gp120

Germ line divergence (%)

SequenceIdentity

withPG04 (%)

# Reads110K reads of HC

from a single donor

Sequencing reveals that divergent antibodies are rare

SequenceIdentity

withPG04 (%)

# Reads110K reads of HC

from a single donor

SequenceIdentity

withPG04 (%)

EffectiveNon-effective

Sequencing reveals that neutralizing antibodies are divergent

110K reads of HC from a single donor

SequenceIdentity

withPG04 (%)

Sequencing reveals maturation lineages

Same CDR H3 Family

SequenceIdentity

withPG04 (%)

Sequencing reveals maturation lineages

Same CDR H3 Family

GoodNeutralizer

Cross-donor phylogenetic analysis can identify

neutralizing antibodies

High performing antibodies from other patients

56 mutations

41 mutations

23 mutations

93 mutations

VRC03High

Performing

germ line

High performingintermediates

could form the basis of an

effective HIV vaccine

HIV-1 Antibodies

Technology

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HIV-1 Western Blot Cambridge Biotech · The Cambridge Biotech HIV-1 Western Blot Kit, when used as directed in this insert, will detect antibodies to HIV-1 when present in human serum,

HUMAN IMMUNODEFICIENCY VIRUS TYPE I - QualTex · PDF fileHUMAN IMMUNODEFICIENCY VIRUS TYPE I ... detection and identification of antibodies to Human Immunodeficiency Virus Type 1 (HIV-1)

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Ability To Develop Broadly Neutralizing HIV-1 Antibodies ... · Laboratory), 1 Modderfontein Road, Sandringham 2131, South Africa. E-mail ad-dress: lynnm@nicd.ac.za The online version

Monoclonal antibodies (HIV) Cross-reactivity … four anti-HIV-2NIHz mAbswere tested for reactivity with cells infected with different HIV-1, HIV-2, and SIV isolates by using an indirect

Monoclonal antibodies (HIV) Cross-reactivity withHIVtype 1 ... · virus (HIV) type 2 isolate andweretested for reactivity with different HIV-1, HIV-2, and simian immunodeficiency

FPO - Home | INSTI · 2020. 6. 9. · CLIA-waived, INSTI® HIV-1/HIV-2 Antibody Test is an in vitro qualitative test for the accurate detection of antibodies to Human Immunodeficiency

OraQuick ADVANCE Rapid HIV-1/2 Antibody Test Use • The OraQuick ADVANCE® Rapid HIV-1/2 Antibody Test is a single-use, qualitative immunoassay to detect antibodies to Human Immunodeficiency

Prevention of HIV-1 Infections by Mucosal Delivery of …. MPT Poster.pdf · Prevention of HIV-1 Infections by Mucosal Delivery of Anti-HIV Antibodies using Human Vaginal Lactobacillus

Increasing access to HIV diagnosis · Early diagnosis in infants and young children is a major challenge. Infants born to mothers infected with HIV-1 may have maternal antibodies

ULTRA Product Line · Competitor A 4.5 5 Internal data and data from commercial panels Genscreen™ ULTRA HIV Ag-Ab Assay EIA for Detection of HIV Antigen and HIV-1 / HIV-2 Antibodies

PIPELINE OF HIV MONOCLONAL ANTIBODIES FOR … · 17/04/2018 · 4/17/2018 1 PIPELINE OF HIV MONOCLONAL ANTIBODIES FOR PREVENTION OF HIV Barney S. Graham, MD, PhD Deputy Director Vaccine