Microbiology training module--finger dab& higeine

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Quality Control-MicrobiologyTraining on hygiene & finger dab test

Sreenath.S

Site Microbiologist

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Over view of finger dab & hygiene• Cleanliness of the personnel

• Maintenance of hygiene

• Proper hand sanitization

• Disinfectant cleaning

• Hand Swabbing

• Use of right sanitizer

• Growth of organisms

• Educating personnel's

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Why Is Hand Hygiene Important?

Hands are the most common mode of pathogen

transmissionHand washing is an extremely

important step

Hand washing significantly removes the bacterial flora on the skin

Hand washing protects you and your patient from the bacterial flora (from

each other) from becoming skin residents

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Hand Hygiene Definitions:

Hand washing−Washing hands with plain

soap and water• Antiseptic hand wash

−Washing hands with water and soap or other detergents containing an antiseptic agent

• Alcohol-based hand rub−Rubbing hands with an

alcohol-containing preparation (used after hand washing)

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When to Wash:• Visibly dirty

• After touching contaminated objects with bare hands

• Before glove placement and after glove removal

• Visibly dirty

• After touching contaminated objects with bare hands

• Before glove placement and after glove removal

Hand washing before and after each operation is the single most important hygiene measure for reducing or preventing the spread of contamination

Hand washing before and after each operation is the single most important hygiene measure for reducing or preventing the spread of contamination

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Rings and Jewelry

• Hand jewelry should not be

worn as it may tear gloves

and harbor bacteria

− Studies have demonstrated that

skin underneath rings is more

heavily colonized than

comparable areas of skin on

fingers without rings

− the more rings worn, the greater concentration of organisms

• Hand jewelry should not be

worn as it may tear gloves

and harbor bacteria

− Studies have demonstrated that

skin underneath rings is more

heavily colonized than

comparable areas of skin on

fingers without rings

− the more rings worn, the greater concentration of organisms

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Fingernails

• Can affect the integrity of gloves

• Can also harbor bacteria

• Keep fingernails SHORT!

− Avoid artificial nails

• Can affect the integrity of gloves

• Can also harbor bacteria

• Keep fingernails SHORT!

− Avoid artificial nails

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Wash hands (or use and alcohol based rub) before glove placement and after glove removal..

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Hand Hygiene: Frequently Missed AreasHand Hygiene: Frequently Missed Areas

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Cleanliness of the personnel’s• Always clean your hands before entering into manufacturing area

and where ever it is to be followed.

• Disinfectant the gloves during operation.

• Personnel Hygiene

• Illness

− Staff with illness or open lesions should not handle starting materials, intermediates or finished products

• Adverse conditions

− operators trained to recognize risks

− willingness to report illness to the area supervisor

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Personnel Hygiene

Health examinations

–On recruitment for direct operators , repeated on regular basis

•Training - check

– induction training for new operators includes basic personal hygiene training

–written procedures - to wash hands before entering a manufacturing area

–signs in changing rooms to reinforce hand washing

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Personnel Hygiene

•Contact between product and operator

avoid direct contact

if direct handling unavoidable, gloves should be worn

check glove disinfection (for sterile

production) and disposal

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Personnel Hygiene

•Clothing and changing facilities

−check changing rooms (hand washing, towels or hot air hand dryers)

−check if used clothing stored in separate closed containers while awaiting cleaning

−laundering of clean area clothing

must be to SOP and in

appropriate facility

−check for procedure for sterilizing

and storing clothing for use in

sterile area

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FINGER DAB TESTFINGER DAB TEST• Frequency:

• Once in six months

• Sampling plan: (Random sampling)

• Persons working in the manufacturing area are selected randomly from each section on the day of the test.

• Limits:

• For Bacteria: Standard limit: NMT 100 cfu/Personnel

• Alert limit: NMT 50 cfu/ Personnel.

• Action limit: NMT 75 cfu/ Personnel.

• Fungi: Should be absent

• Pathogens: Should be absent

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FINGER DAB TEST DEMOFINGER DAB TEST DEMO

SWAB TAKEN BEFORE HAND WASH SAMPLE UNDER MICROBIAL

TESTING

AFTER INCUBATION COLONIES ARE IDENTIFIED

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FINGER DAB TEST DEMOFINGER DAB TEST DEMO

SAMPLE TAKEN AFTER HAND SANITIZATION

SAMPLE UNDER MICROBIAL

TESTING

AFTER INCUBATION NO COLONIES ARE IDENTIFIED

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Taking off the first glove:

1.

2.

3.

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4.

5.

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Taking off the second glove:

1.

2.

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3.

4.

5.

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Disposal:

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THANK You………THANK You………

Any Query ????????