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Pharmaceuticals in the Environment.By:Abdel-Rahman Ragab
Outlines.
1- why interests increased for presence of pharmaceuticals in the environment ?
2- Pathways for entry into the environment.
3- Environmental detection of pharmaceuticals.
Part 1 : why interests increased for presence of pharmaceuticals in
the environment ?
Vet diclofenac catastrophe.In 1980s there were up to 80 million eagles in India; but today the population numbers only several thousand.99% decline of eagles species in south Asia.
Effects on aquatic organisms.1-tricyclic antidepressants (ppb levels) : affect on development ,spawning and behaviors in shellfish, ciliates and other aquatic organisms.2-calcium-channel blockers : affect on sperm activity of some aquatic.3- Antiepileptic drugs : trigger extensive apoptosis in the developing brain of human neurodegeneration.4-ppm and sub-ppm levels of various drugs (NSAIDS, glucocorticoids, anti-fibrotics) affect collagen metabolism in teleost fish, leading to defective/blocked fin regeneration.5- Multi-drug transporters (efflux pumps) are common defensive strategies for aquatic biota — possible significance of efflux pump inhibitors in compromising aquatic health?
Drugs Having Double Uses: Medicinals and Pest-Control AgentsIt poses the possibility that these alternative uses serve as additional sources for their introduction to the environment. Examples:(4-aminopyridine, warfarin,triclosan,azacholesterols,antibiotics ,acetaminophen,caffeine,NSAIDs and pentobarbital).Acetaminophen : analgesic and useful for control brownTree snake
Antibiotics and endocrine disruptors.-antibiotics cause bacterial resistance .-endocrine disruptors : interfere with hormone and disrupt its function ex: estrogen in contraceptive pill.
A total of US$954 billion was spent on prescription medications globally in 2011 alone. in 2002 95 contaminant measured in us. 94cg
Figure 1 Number of publications per year for the period 1996–2014 retrieved using Web of Knowledge™ and the search words “pharmaceutical*” and “waste water.”
Part 2: Pathways for entry into the environment.
Pathways to sewage.
Wastes of Pharmaceutical manufacture
sewageUrine &
feces
bathing
disposing
Pathways to Air .
Non inhaled active ingredient
insignificant
completelyoxidized to innocuous compounds such as
carbon dioxide and water
Pathways to soil.
Urine & feces
sewage
disposing into the waste
bin
Wastes of Pharmaceutical manufacture
River/Estuarine/Coastal water
Pathway to water.
Surface water
Sewage treatment
River/Estuarine/Coastal water
Ground waterLandfill
Pathway to water
River/Estuarine/Coastal water
Sewage treatment
Soil
Surface water
swimming
Pathway to biota.
River/Estuarine/Coastal water
Groundwater Drinking water sources
Figure 2 Pathways for entry into the environment.
Part 3: Environmental detection of
pharmaceuticals.
Figure 2: Procedures and techniques used in the preparation and analysis of environmental samples.
Sample collection.Solid sludge.
collection
Placed in glass bottle then freeze-dried and ground to 250 µm sized particle before storage .
Sample collection.-composite sample, grab sampler,24-h sample with weather consideration ( rain and seasonal variation).
bottle types used for the collection of water Samples.
-Type chosen according to analyte of interest and environmental sample . -silanized or not (alkoxysilane molecules).-recovery % & physicochemical properties important .
Amber
Clear glass
PET HDPE
Sample bottle types used for the collection of watersamples in some field investigations of pharmaceutical analytes
Sample storage. Purpose :prevention bacterial growth & analyte degradation.When direct analysis isn't available .Preservative are added ex HCL,HNO3 and H2SO4. 72 h store at 4 °C without preservative.3 weeks at 4 °C 24 h then frozen at -20 °C for 3 weeks
Sample filtration. Purpose : to remove particulate matter with filter paper.Type of filter paper depend on analyte of interest Ex estrogen glass fiber filters
Extraction.Purpose : increase conc of analyte to reach detection limit of device .2 types Solid-phase extraction & Liquid–liquid extraction.Solid –phase extraction is suitable .
Separtion.By high performance (pressure) liquid chromatography (HPLC)
Mass spectrometric detection and quantization
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