nematodes morphology

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Lecture 2Nematode Morphology

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Topics

Size

Shape

Body wall (Outer body tube)

Digestive system (inner body tube)

Reproductive system

Excretory system

Nervous system

Respiratory and Circulatory systems

Definition

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Size

Human and Animal parasites are big and visible to the naked eyes Ascaris lumbricoides …………………...30 cm

Dracunculus medinensis……………….1m

Placentonema gigantissimma ………...8 m (longest)

Free-living nematodes microscopic Greeffiella minutum…………………….82 µm (smallest)

Plant parasitic nematodes Microscopic, range……………………..0.3 – 2.0 mm

Paralongidorus epimikis ……………1.2 cm (longest)

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Relative Size & Shape

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Shape

Normal shape

Vermiform Sexual dimorphism

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Body regions, Colouration, Segmentation

Head – Continuous or set off

Tail - body portion beyond anus, shapes variable

Appendageless

Colourless

Body wall transparent

No metameric segmentation

All natural openings on one side - ventral side

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Body Organization and Symmetry

Body tubular Outer tube – Body wall

Inner tube – Alimentary canal

Body Cavity – Pseudocoelome

Bilateral symmetry

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Body Wall

1. Cuticle 2. Hypodermis 3. Muscle layer

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Cuticle

– Non-cellular

– Tough but elastic

– Exoskeleton

– Regulates permeability

– Regulates body growth

– Helps in locomotion

– Plays role in respiration

– Plays role in excretion

– Moults four times

– Consists of structural proteins

(keratin, collagen, matricin)

– Chitin absent in cuticle

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Cuticular markings

A.Transverse

Smooth Striated Annulated

Striated Annulated

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Cuticular markings

Caudal alae (Bursa)

Leptoderan Peloderan

B. Longitudinal

Lateral alae

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Hypodermis & Musculature

Hypodermis: Cellular or syncytial, secretes new cuticle after every moult,

forms 4 hypodermal chords

Musculature : Somatic, Specialized

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Musculature

Somatic muscles Attached to body wall

Nvbnvbn

• Specialized musclesAssociated with special organs e.g., stylet, spicules

A. Platymyarian B. Coelomyarian C. Circomyarian

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Alimentary Canal – The Inner Body Tube

Stomodaeum – The Fore gut

Oral aperture (Mouth)

Stoma ( Buccal cavity)

Oesophagus (Pharynx)

Cardia (Oesophago-intestinal valve)

Mesenteron – The Mid gut

Intestine

Proctodaeum – The Hind gut

Rectum

Anus

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Stomodaeum - Oral aperture

En-face view

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Microbivorous Predator Phytophagous

Stomodaeum - Stoma16

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Cephalic Sclerotization & Stylet17

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Stomodaeum - The StyletStomatostylets in Tylenchids

Conus

Shaft

Knobs (

3)

Lum

en

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Stomodaeum - The Oesophagus

Corpus

Postcorpus

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Stomodaeum - PPNs

No Gland overlap Gland overlap

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Feeding Tube21

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The Mesenteron and Proctodaeum

MesenteronIntestineWall made up of single layer

of epithelial cells;

inner margins lined with microvilli

ProctodaeumRectumIn males, intestine joins vas deferens

to form common tube – cloaca

AnusFemales have a separate opening;

pore-like on ventral side

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Reproductive System

Nematodes are dioecious

Both sexes look alike

Reproductive system is tubular

Males v/s Females

• Males are slightly shorter

• Tail end curved ventrally

• Possess secondary sexual organs

• Spicules

• Gubernaculum

• Bursa

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Female Reproductive System

Ovary

Germinal zone: Production of oogonia

Growth zone: Oogonia increase in size

Oviduct

A narrow passage for oogonia

Spermatheca

Store sperms; oogonia get fertilized

when pass through

Uterus

Columellar glandular cells – deposit

egg shell around fertilized egg

Vagina

Expulsion of egg

Vulva

Slit-like on ventral side; help in egg

laying and copulation

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Female Reproductive System

Terminology

No. of genital tracts• Monodelphic

One

• Didelphic

Two

Direction of genital tracts• Prodelphic

Directed anteriorly

• Opisthodelphic

Directed posteriorly

• Amphidelphic

One directed anteriorly,

second posteriorly

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Male Reproductive System

TestisProduction of spermatogonia

Seminal vesicleStorage of sperms till mating

Vas DeferensPassage for sperms, merges with intestineto form cloaca

CloacaCommon tube for digestive and reproductive systems

Cloacal apertureCommon opening for both systems

SpiculesSclerotized, a pair, movable, mating organ

GubernaculumPlate-like, not movable, guides the movement of spicules

BursaExternal cuticular extensions on lateral side, a pair, leptoderan or peloderan, hold female during mating

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Excretory System

Phylum Nematoda divided into two classes based on the type of excretory system

Function speculatory, may be secretory, osmotic regulation etc.

Canalicular or Tubular typePresent in Class Secernentea

Basically H–shaped, variable

Two main longitudinal excretory ducts running

in lateral hypodermal chords

Joined in oesophageal region by a transverse duct

A terminal duct arises and opens on ventral side -

the excretory pore

Glandular typePresent in Class Adenophorea

Single (Renette) cell leads to an excretory duct

and opens outside through pore

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Nervous System

• Main nerve centre (Brain)

is a ring called Nerve Ring

or Circum-oesophageal commissure

encircling the isthmus region of

oesophagus

• Main nerves arise from nerve ring

and run anteriorly and posteriorly

in the hypodermal chords

• These longitudinal nerves are

interconnected at regular intervals

throughout length

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Sense organs

• Chemoreceptors(to perceive the sense of chemicals in vicinity)

AmphidsA pair, opening on lateral sides in head region,

help in locating plant roots

(Host finding/Chemotaxis)

PhasmidsA pair, opening on lateral sides in tail region,

enlarged phasmids are called Scutellae

• Tactoreceptors(to perceive the sense of touch)

Papillae/SetaeCephalic

Cervical

Caudal

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Other Systems

Respiratory System

Well developed organs lacking

Oxygen required for aerobic PPNs diffuses through cuticle

Circulatory System

Well developed organs lacking

Pseudocoelomic fluid serves as the means of transport since it bathes different organs

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Definition

Nematodes are triploblastic,

bilaterally symmetrical,

unsegmented,

pseudoceolomate invertebrates,

with four main hypodermal chords,

a triradiate oesophagus,

circum-oesophageal nerve ring,

and lacking specialized organs for

respiration and circulation;

they have tubular gonads

which open separately in females

but join alimentary canal in males

to open through a cloacal aperture.

Gross Morphology

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