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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Workshop 2“Research on Genetic Resources
and the Genome: A Need for regional Partnership. The Example of Sugar Cane”
Louis-Marie RABOINUMR PVBMT CIRAD – University of La Réunion
Meeting for the Advancement of Research in the Indian Ocean RegionSaint-Denis de la Réunion – 3-5 June 2003
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Research on Plant genome
Biodiversity evaluation of genetic resources
⇒Management and conservation of geneticresources
⇒Valorisation of genetic resources
Plant breeding
⇒New methods (MAS, GMOs…)
⇒Plant variety protection (UPOV)Seeds of Traditionnal Reunionese vegetables «légumes lontans» (M.Roux-cuvelier)
Sugarcane chromosomes (A. D’Hont)
0 mSSCIR4m5
25,5 R137Ss td25,6 aagctt1927,2 atctt2228,2 aaccac628,8 attcag30,7 Rus t res is tance 32,9 actc tg9r32,9 CDSR2932,9 aaccgt32,9 aagcta23
33 acgctt1O36 acgctt17
59,2 acgcag6
0 accctt16
16,5 RGA024Ss ta19,4 RGA183Ss ta20,3 mSSCIR52m6
24 aggcat526,3 aagcat2333,5 ac tca t4
44 mSSCIR13m2
52,3 RGA313Ecoe52,3 RGA313Ecob
75,2 acgcag7
Genetic map of sugarcane cultivar R 570 Maize Hybridization (J. Dintinger)
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
On the way back : A good knowledge of genes will facilitate the exploration, management and use of available genetic resources
Towards the localisation, identification and isolation of interesting genes
1 BAC100 kb
1 chromosome100 000 kb
Sequence
Genetic map BAC contigs
Genome10 000 000 kb
1 Gene1-5 kb
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
INTEREST OF MODEL PLANTS
recombinants
S. spontaneum
S. officinarum
Maize2n=20
5 500 Mbp
Syntenic Relationships
Sugarcane2n=c.a.115
10 000 Mbp
Arabidopsis2n =10
660 Mbp
Rice2n =24
860 Mbp
Genoplante
(Collaborative projects)
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
High throughput technologies« more markers, more samples, less time »
Experimentation
Knowledge of genetic resources
Identification of genes
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Maize
-Maize germplasm from Reunion: a unique source of resistance to viral diseases
-Study of genes involved in those resistance and introgression in adapted lines
Examples of Research Programmes conducted in Plant Protection Pole (3P)
Vanilla (project)
- Study of genetic diversity of a collection of Vanilla Planifolia (cultivated in Reunion)
Tomato
- Study of resistance to viruses and bacteria
Tomato yellow leaf curl virus
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Sugarcane
-Sugarcane : number one crop in Reunion
-Resistance to sugarcane diseases has been a concern for CERF since its creation in 1929
-Exploration of the very complex genome of sugarcane with the objective to identify genes involved in sugarcane resistance to its different pathogens and pests
Gumming rust
Leafscald
Examples of Research Programmes conducted in Plant Protection Pole (3P)
Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
CSIRO,BSESAustraliaCytogenetic
mappingCIRADet Univ.Reunion(UMR PVBMT)
Genetic mappingDisease résistancesugar content
CERFBrasilESTR genesBAC sequencing
Barbados Sugar content taggingIntrogression
China
Europe, projet riz plante modelFrance, génopole, génoplante, génoscope, inra, Ird
CIRAD- BiotropFingerprintingGenetic mapping, Gene tagging, cloning, cytogenetic
United statesBAC library
ISSCTInt. Soc. Sugarcane Technol.
ICSBInt. Cons. Sugarcane. Biotech.
SASEX South AfricaSmut resistanceLinkage disequilibrium
MSIRIMauritius
Genetic mapping
Example of the collaborative network of CIRAD sugarcane genome analysis project
India
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Meeting for the Advancement of Research in the Indian Ocean Region - Saint-Denis de la Réunion – 3-5 June 2003
Concluding remarks
Work in collaboration is more efficient to face high cost and heavy task of this research (regional collaborations should be strengthened)
Research programmes should be focused
-on regional problematics (Sugarcane, vanilla…)
-on the study of original regional genetic resources (either natural or domesticated)
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