U-PLEX Human Grp1 Custom Quick Guide - Meso Scale/media/files/handout/u... · Prepare 2X Read...

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Reagent Preparation

Prepare Conjugated Capture and Detection Antibodies

Prepare the U-PLEX Plate

Step 1 Create the Individual U-PLEX-Coupled Antibody Solutions

Step 2 Prepare the Multiplex Coating Solution

Step 3 Coat the U-PLEX Plate

Prepare Calibrator Standards

Reagent Preparation

Reagent Preparation/Assay Protocol Dilute Samples

Prepare Detection Antibody Solution

Prepare 2X Read Buffer T

Step 1 Add Samples and Calibrators

Step 2 Wash and Add Detection Antibody Solution

Step 3 Wash and Read

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