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Topic 8: DNA Exercise I: Isolation Analysis Plasmid DNA
Topic 7: Cellular Genetics.Exercise II Mitosis Cell Cycle
Exp: Cell cycle onion tips
Structure of Escherichia coli.
Two strains of E.coli: pBR322 and pUC18.
Purification Plasmid DNA 1:
Purification Plasmid DNA 1:
Spin and remove most contaminants:
Purification Plasmid DNA 2:
Addition of very small volumes:
DNAse and RNAse treatment:
Preparing the Agarose gel:
Agarose Gel.
Electrophoresis:
Electrophoresis:
Electrophoretic Mobility of Nucleic Acids:
pBR322 pUC18
ET D R ET D R
pUC18 pBR322 ET D R ET R D
MITOSIS: Onion root tip.
Onion root tip.
Onion root tip.
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