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1 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar1
Engineering Nanomaterial Surfaces forBiomedical Applications
Rajesh Kumar
NE 489 Final PresentationWinter 2010
2 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Introduction
o Nanomaterials possess unique physical and chemical properties
o Functionalized nanomaterials & synthetic ligandso Applications in:
o Bio-molecular sensingo Biological imagingo Drug delivery vehicleso Disease therapyo Scaffolds in tissue engineering
o Surfaces play a critical role in function
3 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Biomedically Important Materials
o Metal nanoparticles, eg. Au and Ag NPso Easy to prepare, stable, unique optoelectronic properties
o Quantum dotso Immunoassays for proteins and other analyses
o Magnetic nanoparticleso Eg. Iron oxides. Motion through external B field
o CNTso Good electrical and thermal conductivity, mechanical
strength and chemical stability
4 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Typical Nanomaterials
5 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Nanomaterial Surface Functionalization
o Non covalent: Physisorptiono Bonding through electrostatic interactions, hydrogen
bonding, and hydrophobic interactionso Surface coating for stabilizing individual NPso Steric repulsion inhibits agglomeration
o Covalent: Chemisorption
6 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Modification of nanomaterial surfaces
7 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Typical Complementary Functional Groups
8 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Photoinitiated Coupling Chemistry
o Carbohydrates are complex in structureo Hard to chemically derivatizeo But still important class of biomolecules
o Hence, use PFPA-functionalized NPso Then use them to covalently couple carbohydrates using UVo Synthesize by adding PFPA directly to NP solutiono Coated NPs are stable and homogenous in solutiono Can use same process for attaching polymers
9 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Gold Nanoparticles
o PFPA-functionalized Au NPs of 20nm in diameter
o Covalent attachment of carbohydrate ligand using UV activation (80% surface coverage using TGA)
o Developed into a calorimetric biosensor for probing carbohydrate-protein interactions
o Treated with Con A target protein
10 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Gold Nanoparticles Before/After Coupling
11 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Gold Nanoparticles Before/After Coupling
12 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
• Conclusions
o Nanomaterials and surface treatment are widely usefulo Applications in analysis, sensing, imaging, and diagnosticso Careful choice of ligands
o To prevent agglomerationo For molecular recognitiono Defining molecular properties of NMso For ligand couplingo For fixing polarity and solubility
13 NE489 Nano-Biomaterials Winter 2010 Rajesh Kumar
Thank You
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