Phylogenetics of the grass family (poaceae)

PHYLOGENETICS OF THE GRASS FAMILY

(POACEAE)

Vishnu Mohanan

Supervisor: Dr. Trevor Hodkinson

WHY GRASSES?

Biodiversity

Over 9000 species

Pioneer species and establishment of ecosystems

Economic

70% of all food crop

Construction

WHY PHYLOGENETICS?

How the group is organised

Improved resolution when used in conjunction with

geographical and morphological data.

Major diversification events

Eg: Emergence of woodiness in Bamboos

Resolution of taxonomic conflicts

Similar morphology, different group?

Plantae Angiosperm Monocot Poales Poaceae

OUR STUDY

Sequence grass DNA

Huge species number

Generic level analysis

Asymmetry in phylogenies

METHOD

PCR Cleanup

Extraction and purification

Electrophoresis gel

Amplification of gene region

using PCR

Collection and Storage

Cycle Sequencing

Cycle Sequencing cleanup

Fragment Analysis using Genetic Sequencer

Electrophoresis gel

Yes No

AGAROSE GEL ELECTROPHORESIS

Total DNA

PCR Products

ELECTROPHEROGRAM

SEQUENCES ATTAINED

18 sequences attained

12 Phylostachys species

2 Fargesia species

1 Pleoblastus species

ALIGNMENT

Aligned by eye in PAUP

PHYLOGENETIC TREE

Well resolved BEP clade

Bambuseae as a

monophyletic tribe

Olyreae sister to

Bambuseae

Pooideae sister to

Bambusoideae

BEP Clade

Bambusoideae

BAMBUSEAE

Separation in

geography

Temperate bamboos

Phylostachys

Fargesia

Pleoblastus

Paleotropical

Neotropical

COMPARING TREES

BEP Clade

Bambusoideae

DISCUSSION

Ancestral bamboo herbaceous or woody?

Woodiness has evolved independently number of times

According to the results, likely that ancestor herbaceous

Olyreae remained herbaceous

Bambuseae developed woodiness

Conflicts with published studies

Further sampling of Olyreae

DISCUSSION

Storage of plant material in liquid CTAB viable?

High degradation of DNA material

Hard to work with

Unreliable PCR amplification

ACKNOWLEDGEMENTS

Dr. Trevor Hodkinson

Postgraduates: Ruth, Muriel and Caoimhe

Trish!

Fellow ‘UREKA’ns

Dr. Daniel Kelly, Fiona Moloney and Dr. Martyn Linnie

Luke McNally, our fearless leader

Science Foundation Ireland

QUESTIONS?

Phylogenetics of the grass family (poaceae)

Documents

Six Plant Families of Economic Importance - David Bogler€¦ · Six Plant Families of Economic Importance . Six Top Agricultural Crop Families • Grass family (Poaceae)—rice,

INFLUENCE OF CHEMICAL, CULTURAL AND MECHANICAL …ufdcimages.uflib.ufl.edu/UF/E0/04/29/29/00001/chaudhari_s.pdf · Para grass is an invasive, perennial grass that belongs to the Poaceae

Poaceae Sistemática Vegetal

Identification of the grass family (Poaceae) by using the ... · Poaceae is 4th largest family of flowering plant while among monocotyledon it is at 2nd position. There is a great

Poaceae (Gramineae)

Evolution of Delphacidae (Hemiptera: Fulgoroidea ...delphacid.s3.amazonaws.com/5509.pdf · combined-evidence phylogenetics reveals importance of grass host shifts JULIE M. URBAN1,

2018 Fall Plant Sale - ncbg.unc.eduncbg.unc.edu/uploads/files/2018_FallSale_Images.pdf · Poaceae Grass Family Perennial Grass/Grasslike White Sep, Oct 3-9 ft. Sun Dry, Average ERGI

Laboratory 22: “Graminoid” Monocotsib.berkeley.edu/courses/ib168/LabHandouts/Lab22Handout.pdf · terminology used in this group of plants (particularly the grass family Poaceae)

Grass Family (Poaceae or Graminae)

Family Poaceae

Phylogenetics - University of Wisconsin–Madison · phylogenetics is compounded by issues of “time”and “space”and “form”. Phylogenetics. Phylogenetics In natural and

Created in Uganda, February 2016 ... · FACTSHEETS FOR FARMERS Created in Uganda, February 2016 Rottboellia cochinchinensis Recognize the problem Family: Poaceae (grass family) Common

Central Piedmont Community Collegejohn.royals.people.cpcc.edu/Turfgrass Identification.pdf25 tribes, 600 genera and 2500 species, the grass family (Gramineae or Poaceae) is one of

Poaceae in Catalonia - lap.uab.cat · 7th ICA, Montebello, 2002 Poaceae pollen in Catalonia 2 Catalonian grasses •296 grass species are cited of Catalonia1. •They have an ubiquitous

Florida Native Plant Society...Problema byssus), skippers. Deer eat the hard, yellow, corn-like seed produced by this grass. Incidentally, both corn and Gama Grass belong to the Poaceae

Unit II: Poaceae (Gramineae) · Poaceae (Gramineae) : Grass Family • Large and ubiquitous family of monocotyledonous flowering plants known as grasses • Name “Poaceae” was

Next Generation Phylogenetics Phylogenetics of Pathogenic

Polyphyly of the grass tribe Hainardieae (Poaceae ...€¦ · The economic and ecological importance of the grass sub-family Pooideae (Poaceae) for humans and animals has induced

Poaceae The Grass Family Alex Stalboerger. Poaceae 900 Genera 9,000 – 10,000 species of “true grasses” Monocot, flowering plants Comprise 20% of vegetation

FAMILY POACEAE (GRASS FAMILY)