Optical Cryoimaging of Rat Kidney and the Importance of Chromosome 13 in Salt-sensitive Hypertension...

Optical Cryoimaging of Rat Kidney and the Importance of Chromosome

13 in Salt-sensitive Hypertension

F. Salehpoura, T. Kurth b, A.W. Cowley, Jr b, M. Ranji*a

a Department of Electrical Engineering, University of Wisconsin Milwaukeeb Department of physiology, Medical College of Wisconsin, Milwaukee

OBJECTIVE AND INTRODUCTION

• Hypertension is the biggest risk factor for the development and progression of stroke, heart failure, and end-stage renal disease.

• Excess dietary salt consumption plays a causal role in the development of salt-sensitive hypertension and renal oxidative stress and injury.

• In this study we used fluorescence imaging in cryogenic temperature to evaluate whether the increased consumption of salt contributes to excess oxidative stress and salt-sensitive hypertension.

METHODOLOGY

A/D Digital I/O Board

Frame Grabber

1)Lens2)Stepper Motor3)CCD Camera4)Tissue Block5)Microtome Blade6)Excitation Filter Wheel7)Emission Filter Wheel8)Ultra Low Freezer9)Excitation Source10)Power Supply

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• NADH and FAD autofluorescent images from each group of kidneys, were processed to extract NADH RR values using MATLAB.

• Composite images were created using all image slices from cryoimager for each tissue, for both NADH and FAD signals.

• The image stacks were analyzed in MATLAB to measure the 3-D max projection NADH RR of tissue and their histograms.

RESULTS AND CONCLUSION

RESULTS AND CONCLUSION