NO. 18 都市部花粉アレルゲン特性調査プロジェクト

PowerPoint Presentation:

In Japan, Japanese cedar (Cryptometria japonica) and cypress (Chamaecyparis obtusa) pollens are considered to be the major unique allergens which can give rise to severe pollinosis and their extent of dispersal is quite wide, especially in the urban area. Cry j 1 is a causative substance of Japanese cedar pollinosis, and it may deteriorate by Cry j 1 invasion to a lower respiratory tract. We observed airborne particles containing Cry j 1 by an immunofluorescence technique using a fluorescence microscope. We evaluated a Cry j 1 reacted with various air pollutants by liquid phase reaction. My study will pay attention to pollen morphological observation, allergenic measurement of airborne pollens and cross-antigenicity evaluation in Japanese urban areas, also including the investigation of various pollens in Chinese urban areas. Morphological changes of pollens will be observed by an optical microscope, a fluorescence microscope and a scanning electron microscope to confirm the existing evidences of visualized suspended particulate matter containing the Japanese cedar and cypress pollen allergens.
Abstract
ELISA3-
Fig. 2. The result of One-Side ELISA method (n = 3).
Cry j 11.1 m
5 (< 1.1 m) 3-
: (AHV)
: 566 L/min
: 71
: 1 (> 7.0 m)2 (3.3 ~ 7.0 m)5 (< 1.1 m)


: (AHV)
: 566 L/min


BSA/PBS100 L372()
Tween 20PBS (TPBS)PBS3- (PBS1 : 100) 100 L372
TPBSPBSIgG (PBS1 : 5000)100 L371
TPBSPBSo-100 L30
*
0
1
2
3
4/12-4/184/20-4/26
Polyclonal antibody
Cry j 1 concentration (ng/m
3
3
0
1
2
3
4/12-4/184/20-4/26
Monoclonal antibody cry j 1
0
1
2
3
4/12-4/184/20-4/26
Polyclonal antibody
Polyclonal antibody
Cry j 1 concentration (ng/m
3
3