Ninhydrid Test of Protein

>> 0 >> 1 >> 2 >> 3 >> 4 >>

*To proof there is free amino acids in protein

*Protein + Ninhydrin + Heat Purple

complex of soul

WHAT IS

THE NINHYDRIN

TEST OF

PROTEIN

Page 5

Ninhydrin

Ninhydrin (2,2-Dihydroxyindane-1,3-dione)

is a chemical used to detect ammonia or

primary and secondary amines. When

reacting with these free amines, a deep blue

or purple color known as Ruhemann's

purple is evolved. Ninhydrin is most

commonly used to detect fingerprints, as

amines left over from peptides and proteins

(terminal amines or lysine residues)

sloughed off in fingerprints react with

ninhydrin.

Protein

• Proteins are complex, specialized

molecules composed of carbon,

hydrogen, oxygen, and nitrogen. Many

proteins also contain sulfur. The building

blocks of proteins are the amino acids.

* Rack tube

* A test tube

* Pipette drops

*Heater

* Tube clamps

* Albumin 2%

*Gelatin 2%

* Pepton 1%

*Ninhydrin reaction 0,1%

*3 provided a clean test tube and

dry, 2ml subtance test

* Added 5 drops of ninhydrin

reaction

*Then, burn on during ±5 minute

*Seen the colour results

No. Test Subtance Result Ninhydrin

Test

Free amimo

acid (+/-)

1. Albumin 2% Purple +

2. Gelatin 2% Purple +

3. Pepton 1% Purple +

>> 0 >> 1 >> 2 >> 3 >> 4 >>

CONCLUTION

From the results of an experiment in getting results :

• Albumin + ninhydrin solution + heat purple

• Gelatin + ninhydrin solution + heat purple

• Pepton + ninhydrin solution + heat purple

So the solution is a positive reaction is Albumin, Gelatin

and Pepton.

*

Leader Winna Rismala Yeniar Moderator Riyafi Rosmiati Member Annisa Nurul Chaerani

Dewita Novitasari

Thank’s to Mr. Ryan & all my friend

Design By

Annisa Nurul Chaerani

SEKOLAH TINGGI ILMU KESEHATAN

JENDERAL AHMAD YANI 2010