View
5
Download
0
Category
Preview:
Citation preview
Rudolf Valenta
Div. of Immunopathology
Department of Pathophysiology
Center of Pathophysiology, Infectiology & Immunology
Medical University of Vienna, Austria
Nahrungsmittelallergien
www.oegai.org
www.meduniwien.ac.at/
allergy-research-program
www.meduniwien.ac.at/
allergy-research-christian-doppler
Adverse reactions to food
Toxic Non Toxic
Non Immune Mediated Immune Mediated
Enzymatic
Pharmacological
Undefined
IgE
Mixed
Non IgE
Classification of food intolerance
Clinical manifestations of adverse food reactions
1. Skin reactionsContact urticaria
Urticaria / angioedema
Flush, pruritus
Oral allergy syndrome (local itching and tingling and/or edema of lips,
tongue, palate and pharynx)
Protein contact dermatitis
Atopic eczema
2. Reactions of respiratory systemRhinoconjunctivitis
Asthma bronchiale
Laryngeal and/or pharyngeal edema
Hoarseness
3. Reactions of the gastrointestinal tractAbdominal pain
Abdominal distension
Nausea, vomiting
Gastritis
Gastroenteritis with diarrhea
Anorexia
Flatulence
Colitis
4. Reactions of the cardiovascular
systemCardiac dysarhythmia
Hypotension
Vascular collaps
Anaphylactic shock
4. Reactions of the cardiovascular
systemCardiac dysarhythmia
Hypotension
Vascular collaps
Anaphylactic shock
Urticaria
Angioedema
Atopic Dermatitis
Mechanisms of allergic inflammation
Larche M, et al., Nat Rev Immunol 2006, 6:761-71
Valenta R, et al., Adv Immunol 2004, 82:105-53
Diagnosis of type I allergy
Provocation testing Detection of allergen-
specific IgE antibodies
ALLERGEN EXTRACTS
Allergen
MC
Allergen source
Definition: Allergen source, allergen extract, allergen
Mixture of allergenic
and non-allergenic
components
Allergen extract
Disease-eliciting
molecule
Definition and production of recombinant allergens
DNA sequence
analysis
Expression of the
recombinant
allergen
IgE Immunoscreening
Isolation of
Phage DNA
cloning into a
vector
Isolation of the mRNA
Synthesis of cDNA
Phage library
Infection of E.coli
Allergen source (e.g., pollen,
animal dander, house dust mite....)
Serum IgE
panel of recombinant
allergens
Three-dimensional structures of important allergens
7 8 9 10 11 12
13 14 15 16 17 18
1 2 3 4 5 6
19 20 21 22 23 24
25 26 27 28 29 30
Bet v 1, the major birch pollen allergen:
Predictor for tree pollen (e.g., birch alder, hazel,
hornbeam, oak) and plant food sensitization
apple: Mal d 1
cherry: Pru av 1
alder: Aln g 1
hazel: Cor a 1
hornbeam: Car b 1
celery: Api g 1
carrot: Dau c 1
Kazemi-Shirazi L, et al., Int Arch Allergy Immunol.2002;127:259-68
Reading the IgE reactivity profiles of allergic patients
for the better managment
of allergic diseases
Hiller R, et al., FASEB J. 2002;16:414-6
Skin testing versus chip testing
Skin testing versus chip testing
Recombinant fish parvalbumin for diagnosis
of fish allergy
Reduced allergenic activity of the parvalbumin mutants
Hypoallergenic food allergens for
toleranceinduction and vaccination
Acknowledgements
Thank you for your attention!
Rudolf Valenta
Recommended