Motor mechanisms. Keywords (reading p. 1080- 1086) Bundle, fiber, myofibril, sarcomere Z-line, thick...

Motor mechanisms

Keywords (reading p. 1080-1086)

• Bundle, fiber, myofibril, sarcomere• Z-line, thick filament, thin filament• Actin, myosin, sliding filament model• Molecular basis for filament movement• Troponin, tropomyosin• Sarcoplasmic reticulum• Integration of synaptic signals• neurotransmitters

Motor mechanisms

• Create movement

• Can be cilia, flagella, contractile proteins, muscles

• Will focus on skeletal muscle– Muscle of vertebrates that is under voluntary

control

Structure of skeletal muscle

• Bundle

• Fiber

• Myofibril

• sarcomere

Part of Campbell 6th edp. 1081

Features of muscle cells• # of nuclei- multinucleate; formed by fusion

of embryonic cells

• length - this results in very long cells

Part of Campbell 6th edp. 1081

Sarcomere• Structure gives muscle a striated appearance

• Z line, thick filaments (myosin), thin filaments (actin)

Part of Campbell 6th edp. 1081

Part of Campbell 6th edp. 1081

Sliding-filament model of muscle contraction Part of Campbell 6th ed

p. 1081

Thick and thin filaments slide past each otherPart of Campbell 6th ed

p. 1081

At maximal contraction, there is no space at end of thick filament,

thin filaments overlap

Part of Campbell 6th edp. 1081

Molecular basis for movement of filaments against each other

Part of Campbell 6th edp. 1082

http://www.youtube.com/watch?v=InIha7bCTjM&feature=related

ATP bound, head retracted and unattached

Part of Campbell 6th edp. 1082

Hydrolysis of ATP cocks head

Part of Campbell 6th edp. 1082

Myosin head attaches to actin filament

Part of Campbell 6th edp. 1082

Release of ADP + Pi causes a further conformational change

pushing against the actin filament

Part of Campbell 6th edp. 1082

Binding of ATP to myosin head causes dissociation from actin

filament

Part of Campbell 6th edp. 1082

Cycle repeats and sarcomere shortens

Part of Campbell 6th edp. 1082

Control of muscle contraction by Ca++

• Tropomyosin- blocks the myosin binding sites on the actin filament when muscle is at rest

• Troponin complex-binds calcium and controls the position of tropomyosin

At rest, myosin cannot bind because sites are covered by

tropomyosin

Part of Campbell 6th edp. 1083

http://www.youtube.com/watch?v=WRxsOMenNQM

During muscle contraction Ca++ levels rise. Ca++ binds to troponin which then pulls tropomyosin way from the

binding sitesPart of Campbell 6th edp. 1083

What triggers the Ca++ rise that induces muscle contraction?

[Ca++] regulated by

the sarcoplasmic

reticulum

Campbell 6th edp. 1083

Structure of the sarcoplasmic reticulum

• T tubules - are a network of the fiber plasma membrane that goes deep into the muscle fiber.

• This allows transmission of the action potential into the fiber

Sequence of events leading to muscle contraction

• Motor neuron releases acetylcholine

• Depolarization of the muscle fiber membrane results in action potentials

• Action potentials trigger release of Ca++ from the sarcoplasmic reticulum

• Increased Ca++ allows actin and myosin to slide against each other

Characteristics of other muscle types:

• Cardiac muscle - found only in the heart, striated, gap junctions allow direct electrical signaling between cells

• Smooth muscle - involuntary muscle, meshwork of actin and myosin, can contract more (greater shortening), but with less tension.