ITSP Introduction

Introduction to ITSP

ITSP Introduction

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This presentation is intended to familiarize viewers with

ITSP – Instrument Top Sample Prep.

ITSP is actively in use in clinical, environmental, and toxicology laboratories in the Americas, Europe, and Asia Pacific to prepare samples for LC-MSMS/GC-MS analysis.

On a PAL autosampler the ITSP cartridge can be moved over trays or

vials to facilitate sample clean-up through JIT automated filtration or

SPE (Solid Phase Extraction).

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ITSP is a Consumable Solution, Not an Instrument

The heart of the ITSP system is a patented cartridge containing customer-defined filter and/or SPE media.

ITSP is Instrument Top Sample Prep

JIT – Just In Time – prep the next sample while the previous sample is being analyzed,

which means no analyte degradation between first and last samples, as can be

seen in batch processes using liquid handlers or vacuum manifolds!

ITSP Markets and Applications

• Clinical/Forensic –• Many different compounds of interest from different biological sample matrices

(urine, blood, hair, saliva):• Pain management drugs from patient urine samples,• Vitamin D from patient blood plasma samples,• Forensic drugs of abuse from case urine or corpse blood samples,• Steroids from blood plasma or serum, and• Immunosuppressants from blood plasma or serum samples.

• Environmental/Food and Beverage –• Pesticide residues from food QuEChERS extract clean-up, and• Contaminants from beverage or drinking water.

• Pharmaceuticals –• Small sample volume – “micro-sampling” (2-5µL) – automated SPE of pharma COI’s

(Compounds Of Interest) from biological or botanical sample matrices.

• Emerging Markets –• Legalized marijuana in the US (both potency of – and contaminants in – THC).

Posters and Application Notes at www.ITSPsolutions.com

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Why should a lab consider ITSP?

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ITSP can provide many benefits:• Improve quality of chromatography by improving peak shape and reducing

interferences,

• Extend LC column life by more than 1,000% over dilute-and-shoot methods,

• Reduce risk of carry-over,

• Improve chain-of-custody verification,

• Increase lab throughput,

• Reduce costs of capital equipment,

• Reduce cost of solvent acquisition and hazardous waste disposal –in some cases by 90% or more (ITSP is green), and

• Utilize valuable analytical chemist’s time for data analysis instead oftaking up time for sample prep.

ITSP uses standard SPE protocols, except miniaturized and automated.

ITSP Market Drivers

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• End users in several markets are seeing a general trend from GC toward LC.• We are seeing robust LC/MSMS instrument sales/opportunities.

• Less expensive analytical instruments with ITSP-prepared samples.• Can use 6430’s instead of 6460’s or 6490’s.

• For the global market - 6430’s use nitrogen generators, while more sensitive instruments require ultra-high pressure nitrogen sources.

• No requirement to buy a liquid handler.

• ITSP uses standard PAL hardware and software.

• With labs experiencing tighter budgets, ITSP allows use of lab technologists (BS degree) rather than analytical chemists (PhD degree) to prepare samples and run instruments. The analytical chemists can focus on analyzing data and reporting results.

ITSP reduces costs while improving consistency and quality of sample prep

ITSP Cartridge Components

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PAL Lower Needle Guide(modified for ITSP)

PAL Syringe Needle

SPE Sorbent or Filter Media

The small inner diameter of ITSP needle guide - reduces volume to improve efficiency, - maintains vertical position of ITSP cartridge during transport, and can be - color coded for different sorbents.

Needle Guide

The cartridge septum grips the needle to allow the PAL to transport the ITSP cartridge.

Septum

Media Cup

8mm Crimp SealThe crown of the ITSP cartridge seal mates with the nipple on thePAL lower needle guide to center the needle in the cartridge.

ITSP Cartridge Configurations

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• ITSP SPE cartridges are available in two nominal bedmass sizes:– 10mg (standard) and 30mg (large).

• A large variety of phases are available from many different sorbent manufacturers:• Reverse phase,• Normal phase,• Ion exchange,• Copolymeric (mixed-mode),• “Special” configurations,

e.g. QuEChERS blend, coconut charcoal, and• Custom configurations – tell us what you want.

The autosampler syringe replaces the column sample reservoir above the sorbent bed as found in manual SPE cartridge formats.

The needle penetrates the septum and creates a seal so that the sample or solvent is forced through the sorbent bed by positive pressure when the plunger is depressed.

Solvents and sample are pushed through the SPE sorbent bed by the autosampler syringe plunger at a controlled rate.

The sample is eluted into a collection plate or vial and then injected into the LC injection valve or GC inlet.

Unlike manual SPE methods which require a vacuum manifold, ITSP solvent and sample flow rates can be precisely controlled with the syringe plunger.

How ITSP Performs SPE

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ITSP Hardware Kit for PAL-xt

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ITSP Prep / Waste Station ITSP PAL Lower Needle Guide

The crown of the ITSP cartridge seal mates with the nipple on the ITSP PAL Lower Needle Guide to center the needle in the cartridge.

(HW-KIT-APM outfits two complete SPE stations)

ITSP Hardware Kitfor PAL3

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ITSP Hardware Kitfor QuEChERS

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Solvent Reservoirs

ITSP PAL Accessories

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TrayHolders

ITSP PAL Accessories

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ITSP Prep Trays

ITSP PAL Accessories

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ITSP ElutionTrays

ITSP PAL Accessories

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Sample Trays

ITSP PAL Accessories

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Additional ITSP Resources are on theUSB Drive included in the ITSP Hardware Kit• ITSP Applications

• Published app notes• PAL macros and methods• List of standards and other consumables• Lab workflows (SOP)

• ITSP PAL Objects (for PAL-xt)• Sample ITSP CC Macros and Methods

(for PAL-xt)• ITSP Automated Method Development• ITSP Setup Guide• Other resources – such as catalogs, videos,

and presentations with more information about ITSP solutions.

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19www.ITSPsolutions.com

ITSP Solutions, Inc.706-395-8300855-395-8300 toll free

Kim GamblePresident and CEOKim.Gamble@ITSPsolutions.com

Rick YoungbloodDirector of TechnologyRick.Youngblood@ITSPsolutions.com

Mailing Address212 Northlake DriveHartwell, GA 30643

Shipping Address10 S. Carolina StreetHartwell, GA 30643Mark Hayward

Chief Science OfficerMark.Hayward@ITSPsolutions.com