Gram Stain of a Clinical Specimen of Candida albicans

Gram Stain of a Clinical Specimen of Candida albicans

Gram Stain of a Clinical Specimen of Candida albicans

Courtesy of University of Washington; with permission.

Growth on Sabouraud's Dextrose Media

الشريحه في الصو�رة عن تتحدثالتاليه

• Gram Stain• Gram + stain of cocci arranged in culster

Staphylococcus

Staphylococci Stained in Pus

Gram-Positive Streptococcus

Sputum stained with gram positive

microorganismpus

StreptococcusStaphylococcus

Streptococcus pyogenes = Group A Strep

Carried by many peoplein throat or on skin

often no symptoms

Cause of • strep throat• Impetigo الجلد اصابه• Necrotizing االنسجه موت fasciitis

القاموس منA bacterium that

causes the formation of pus or of fatal septicemias

Streptococcus pyogenes. Left. Gram stain of Streptococcus pyogenes in a clinical specimen. Right. Colonies of Streptococcus pyogenes on blood agar exhibiting beta (clear) hemolysis

Beta hemolysis

Alpha-hemolysis

• Alpha-hemolytic Streptococcus species "Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis.

احفظ االنواع

المسببه مهمه

Viridans group" streptococci, including species such as the Streptococcus mutans, mitis, and salivarius groups display alpha hemolysis. (Also see Figures 15,16 and 23-25).

Alpha-hemolytic Streptococcus species

Bacitracin مضاد اسمsensitivity

Demonstrations and practial

• Principle:– Bacitracin test is used for presumptive

identification of group A للترجيح– To distinguish between S. pyogenes

(susceptible حساس to Bacitracin) & non group A such as S. agalactiae (Resistant to B)

– Bacitracin will inhibit the growth of gp A Strep. pyogenes giving zone of inhibition around the disk

• Procedure:– Inoculate يلقح BAP ( فيها اللي الميديا االجار

with heavy suspension of tested المضاد (organism

– Bacitracin disk (0.04 U) is applied to inoculated BAP الملقحه العينه

– After incubation الحضانه any zone of ,فترهinhibition around the disk is considered as susceptible يعني المضاد هذا من حساسأي) ( قروب

الدائره هي

العالمه الفارقه

Group A

Optochin Susceptibility Test

Optochin resistantS. viridans

Optochin resistantS. viridans

Optochin susceptibleS. pneumoniae

اللي االختبار فكره نفس اختبار راح

هي هنا الحساسه البكتيريا : بسs.pneumonia

Lowenstein-Jensen medium

Lowenstein-Jensen medium

Mycobacterium tuberculosis

Mycobacterium tuberculosis

Name of its medium

Mycobacterium tuberculosis Ziehl-Neelsen stain

Acid-fast bacilli=redish colour

Aspergillus niger

Culture of Aspergillus niger. Conidial head of A. niger

Aspergillus niger

alpha hemolysis (green zone surrounding colonies). Note the zone of inhibition around a filter paper disc impregnated عليه with optochin. Viridans وضعstreptococci are not inhibited by optochin.

Streptococcus pneumoniae - optochin sensitivity test

Haemophilus Species

Direct smear لطاخه of H. influenzae in CSF in a case of meningitis. Note the intracellular and extracellular pleomorphic االشكال -gram متعددnegative bacilli.