FlowJo 中文实用手册

I FlowJo support@flowjochina.com 400-680-5527

FlowJo FlowJo7.6.5 2012/06/20

FlowJo ......................................................................................................................................1 FlowJo ...............................................................................................................1 FlowJo(Windows) .........................................................................................................1 FlowJo ...............................................................................................................................5

FlowJo ......................................................................................................................6 ...........................................................................................6 .........................................................................................................................10

FlowJo ............................................................................................................13 .................................................................................................13 .....................................................................................................................16 .........................................................................................................................16

FlowJo ....................................................................................................................18 FlowJo..............................................................................18 FlowJo .........................................................................................................18 .....................................................................................................................................24

FlowJo ....................................................................................................................27 .........................................................................................................................27 .........................................................................................................................32 .........................................................................................................................35

FlowJo ............................................................................................................43 .................................................................................................................................43 FlowJo .........................................................................................................44 .........................................................................................................................45

PatrickUnderline

PatrickUnderline

PatrickUnderline

PatrickUnderline

II FlowJo support@flowjochina.com 400-680-5527

FlowJo ....................................................................................................47 .........................................................................................................................47 FlowJo .................................................................................................48 .................................................................................................51 .........................................................................................................................53

PatrickUnderline

III FlowJo support@flowjochina.com 400-680-5527

1 FlowJo support@flowjochina.com 400-680-5527

FlowJo

FlowJoLeonard HerzenbergFACS

90FlowJo

FlowJo

FlowJo

1. PC

Windows XPVistaWindows7

512MB

2.

OSX10.3

512MB

FlowJo(Windows)

http://www.flowjochina.com/content/download


V7.6.5

http://www.flowjochina.com/sites/default/files/7.6.5_X32.exe

FlowJo

FlowJo

NEXT


NEXT

NEXT

Install

Done


a. DongleFlowJo

b. Serial NumberUse

Serial Number

c. Get Trial LicenseFlowJo

Use Serial Number


d. DemoFlowJo

Run in Demo ModeDemoFlowJoDemo

FlowJo

FlowJo


FlowJo

,flowjochina

www.flowjochina.com/sites/default/files/demodata.rar

3 color compCy5PE comp.fcs

1. FlowJo

2. 3 color compFlowJo


3 color comp3 color

comp

Cy5PE comp.fcs


1) XSSC

SSC

2) YFSC

FSC

3)

:


1) XCy5PE:CD4YHistogram

2)

a.

CD4CD4


b.

CD4CD

a.

WORDPPT

b.

SVG

PNGEMFJPG


c. FlowJo

d.


e.

data001.JPGJPG, PNG, GIF, EMF,

SVG, PDF

FlowJoPNG

f.


FlowJo

FlowJo

FlowJo3-color-experiment931115-

B02-Sample01.FCS

1. 3-color-experimentFlowJo

2. 931115-B02-Sample01.FCS

XFSCYSSC


3. XFluor::CD3YFSC

CD3T


4. TXCy5PE::CD4Y

PhyEry::CD8

Q1CD8CD8+ T

Q2

Q3CD4CD4+ T

Q4


TREGCD4/CD25/FOXP3SSC vs. CD4

CD4+CD25 vs. FOXP3

CD25+/FOXP3+CD4+/CD25+/FOXP3+

TREG


FlowJo

FlowJo

FlowJo

1.

2.

3.

FlowJo

FlowJoFlowJo

4

http://www.video.flowjochina.com/v75%20Compensation_conv.html

1. FlowJo

3 color compCy5PE comp.fcs, FITC

comp.fcs, PE comp.fcs3-COLOR.FCS

Cy5PE, FITC, PE

PatrickHighlight

PatrickHighlight

PatrickHighlight


2.

Sample

FlourFITC comp.fcs


3. 23FlowJo

4.

>


(poor control )FlowJo

1. 1,2,3

FlowJoPE


1) PEcw_Size

2) Clear

2.

1) PEFSC/SSC


2) XPhyEry::aCD8Y

Histogram PEPE

a.

b.

3) PE-PE

PE+


3. FlowJo

3-color comp

>

0Log

0

LogicalFlowJo

3-color comp3-

COLOR.FCS

1. 3-COLOR

XCD3YCD8

gate135.1%


2. XYT

3. T

Change transform values


X-10-30

a.

b. FCS3.0FlowJoFCS2.0

FlowJo


FlowJo

Titration DataCD8Sample 1

Sample 2 ~ Sample 82

1.

Sample 1


XFL1-HYHistogram Sample 1

FL1-H+

RenameCD8+FL1-H-

CD8-

2.


CD8+

MedianParameter

FL1-HCD8FL1-H


(FL1-H):MedianCD8-CD8

FL1-H

3.

Sample 1Sample 2

Sample 2Sample

1


Sample 1

Titration Data

Sample 1

ALTSample 1

Titration Data

Sample 1


1.

Sample 1CD8+CD8-

(FL1-H):Median


2.

Keyword

Antibody Concentration

OK

3.

CD8+ (FL1-H) Median CD8- (FL1-H) MedianCD8

CD8MFI

OK


4.

MFI

1MFI1.

1.

Sample 1


FlowJoTitration Data

FlowJo


2. (Overlay)

1

Titration Data

(1) Sample 1Sample 2

Sample 1

(2) SpecifyControl

Sample 1Sample 1OK


(3)


2

Titration Data

(1) Sample 1CTRL

Sample 1

Sample 1


(2)


(3)

(4) coloringfilled

(5)


FlowJo

Annexin V-FITC/PI

Annexin V-FITC/PI

PSVAnnexin V-FITC

PS

PSPI

PS

AnnexinPI

Annexin V-FITC/PI

AnnexinAnnexin, PI


FlowJo

apoptosisZL-2011-12-20_400.fcs

1. FlowJo

XFSC-A,YSSC-A

2. SSC/FSC


XFITC,AnnexinV-FITCYPE-Texas,PI

1.

2.

Q1(AnnexinV-FITC)-/PI+

Q2: (AnnexinV+FITC)+/PI+

Q3(AnnexinV-FITC)+/PI-

Q4(AnnexinV-FITC)-/PI-


Q2+Q3

AnnexinV


FlowJo

G1

SG2MG1S

DNAG2DNA

M

DNAPIPI

DNARNA

DNA

DNA


FlowJo

cell cycle 5206.001PI

1.

5206.001XFSC-HY

SSC-H


2.

XFL2-W(width)YFL2-A(area)

BDFL2-A

FL2-WFL2-AFL2H

BeckmanXFL3-LIN,PI

YFL3-PEAK,PI


3. FlowJo

G1SG2G1CV

G2CV


http://www.flowjochina.com/content/tutorial-video#cellcycle

1.

RMS

RMS(root mean square error)

RMS3.85

2.

WatsonDean-Jett-Fox

RMSRMS

Could not fit.

Watson

Dean-Jett-Fox


3.

G1G2meanCV

1 G2G12

G1G2G1

2(2G1)G2G1G20.5

2 G1G2

G1mean

G1mean

3 CV

DNAG1G2CV

G1 CV

=G2 CVG2 CV G1 CV

CVCV

CV


1. RMSroot mean square errorRMS

2. G1G1

SS

G2G2

3. G1G1

G2G2

4. CVcoefficient of variationG0/G1

CVCV

G1 cvG1G1

G2 cvG2

Flowjo

FlowJo FlJ FlowJo

FlowJo 中文实用手册

Documents

Introduction to FlowJo Flow Cytometry Core Facility Diana

Dir 605 A1 中文实用手册

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Public Disclosure Authorized 手册第二卷：实施手 …documents.worldbank.org/curated/en/354591486025472708/...传真：+1 202-522-2422 手册 (四) 第二卷：实施解决公司治理纠纷