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8/13/2019 DNA Testing for Personal Identification
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FORENSIC AND MEDICOLEGAL DEPARTMENT
FACULTY OF MEDICINE DIPONEGORO UNIVERSITY
RSUP DR. KARIADI SEMARANGPeriod 23 September 19 Oktober 2013
Mentor: dr. Gatot Suharto, SH, SpF, MKes, DFM
Fasillitator: dr. Donald Rinaldi K.
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Dika Gita Pratama 22010112210012 FK UNDIP
Gita Soraya 22010112210026 FK UNDIPUntari K. 0861050131 FK UKI
Nurul Charla H. 0861050134 FK UKIAaron Y Tehupuring 0961050104 FK UKI
Aldo I. R. Sitanggang 0961050112 FK UKI
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DNA
PersonalIdentification
http://www.google.co.id/imgres?imgurl=http://www.clker.com/cliparts/6/4/7/7/1197106712447482105johnny_automatic_walking.svg.hi.png&imgrefurl=http://www.clker.com/clipart-14727.html&usg=__dE6HqDio0ZunpstXTZ-0R7S2dvA=&h=254&w=600&sz=27&hl=id&start=15&sig2=knv66vJ95adBzP9edL1CYg&zoom=1&tbnid=nusd6ns-vttH5M:&tbnh=57&tbnw=135&ei=fHRJUo6lBInmrAernIGgCQ&itbs=1&sa=X&ved=0CEYQrQMwDg8/13/2019 DNA Testing for Personal Identification
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General Aim
Knowing the function of DNA examination forpersonal identification
Spesific Aim
Definition and classification of personalidentification
Definition, structure, and DNA testingtechniques
The benefits, procedure as well as theapplication of DNA testing in theidentification of personal
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FORENSIC
IDENTIFICATION
is an attempt to determine theidentity of a person for the benefitof forensic, which the interests of
justice
PERSONAL
IDENTIFICATION
One of efforts of the individualdifferences with other individuals
or attempt to determine ownership
of body parts, body fluids or otherbody parts are still good either
been damaged or that have beendestroyed even though the owner
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Whole medicolegalcasses
suspect of muderer
Suspect of rapist
Child identification
Missing kids
Person
Blasting
Burning
Train and airplanesaccident
Flood
Mortality case
Corpse
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Conventional
Fingerprint examination
Visual Method
Document examination
Clothes and jewelry watching
Medical examination
Dental examination
SerologyExclusion
Mutilating identification
Skeletal examination
Modern- Molecular Forensic (DNA Testing)
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DNA is the genetic material that carriesinformation that can be derived.
DNA that usually use are c-DNA and mt-DNA. The most accurate DNA sample is c-DNA
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3 main components : Fosfat chains
deoxyribose Nitrogen base :
adenin (A),
Guanin (G)
Sitosin (C)
Timin (T).
http://id.wikipedia.org/wiki/Polimerhttp://id.wikipedia.org/wiki/Polimer8/13/2019 DNA Testing for Personal Identification
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Movement of DNA to the positivepool when it puts on electric field
GelElectrophoresis
Quick method for DNA duplication
PolymeraseChain Reaction(PCR)
Standart technique to analyze andseparate DNA fragment thenduplicate it
CapillaryElectrophoresis(CE)
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RFLP (Restriction Fragment Lenght Polymorphisms)
Short Tandem Repeats(STR)
Pentanucleotide repeats
Genetic markers based on repetitive sequences
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SNP (Single nucleotidepolymorphisms)
HLA-DQA1
Polymarker (PM)
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Mitochondrial
DNA (mtDNA)
Y ChromosomeMarkers
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mt-DNA is another choiceto examine the DNAsample that cantdetermine by RFLP or STR
Every mothers have theirown mt-DNA
This examination confirma relation between motherand descent.
Y chromosome passed onfrom father to all his sonsso that the DNA on the Ychromosome can be usedto find the descendants ofthe male and is usually
used for cases of sexualcrimes
This examination candetect man profile whenwomen profile had clearwith STRs
Y-SHORT TANDEM REPEATS(Y-STRS)DNA MITOKONDRIA
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Is a result from PCR
application
Its shows the
differences of
pattern each
individual
Probability of error isminimum
The accuracy almost100%
Time-consuming
expensive
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(+) (-)
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To identificate suspect
To determine the criminalitynetwork
its used as secondary evidenceneeds other evidence to support
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There are so many technique for DNAidentification. Every technique has their own
advantages and disadvantages. The techniqueare depends on many factors, example cost,sample, etc. Then finally the result of the
identification can determine the identity of aperson, body parts and body fluids which theinterests of court.
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Definition RFLP is a DNA
polymorphism that occurs
due to variation in thelength of DNA fragmentsafter cut with a specific
restriction enzyme.
Techniques
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This method uses a short marker, repeat pattern
alleles at mikrovarian segment between 3-7 basepairs.
Advantages : damaged samples, limited sample,etc.
Limitation : relatively expensive and limitedPossibility for DNA contamination
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