Diaserve Laboratories GmbH · Catalogue Number MBP1 Clone, Isotype MBP Clone 1, IgG2a Format IgG...

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BioServUKLtdTheInnovationCentre,217Portobello,Sheffield,S14DPTel:+44(0)1142242235Email:info@bioservuk.com

DiaserveLaboratoriesGmbHSeeshaupterStr.27,82393,Iffeldorf,GermanyTel:+49(0)8856803605Email:contact@diaserve.de

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DatasheetAnti-MBPClone1ProductName AntiHumanMBP1

CatalogueNumber MBP1

Clone,Isotype MBPClone1,IgG2a

Format IgG

TestedApplications WB,IHC,ELISA,ICC

Description:MyelinBasicProtein(MBP)isinvolvedintheprocessofmyelinationofnerves.MBPClone1recognizesanepitopeinthe129-138regionofMBP,usefulinclinicaldiagnosistodetectMBPlevels.ProductDetails:Forminstock:IgG,purified–1.0mg/mL.Alsoavailableasunpurifiedsupernatant.Host:MouseSpecificity:ReactswithMBPfromhuman,bovineandrat,recognizingepitope129-138.Fusionpartner:SpleencellsfromimmunisedNIHmicewerefusedwithcellsoftheNS0mousemyelomacellline.Histologypositivecontrol:HumanbraintissueStorage:Storeat+4°Cor-20°C.Avoidrepeatedfreezingandthawing.Shelflife:18monthsfromdateofdispatch.Regulatory/Restrictions:Forresearchandcommercialpurposes.Applications SuggestedDilutionWesternBlot 1:10–1:200Immunohistochemistry 1:10ELISA 1:200–1:1000Immunocytochemistry 1:30002

©BioservUK2017

BioServUKLtdTheInnovationCentre,217Portobello,Sheffield,S14DPTel:+44(0)1142242235Email:info@bioservuk.com

DiaserveLaboratoriesGmbHSeeshaupterStr.27,82393,Iffeldorf,GermanyTel:+49(0)8856803605Email:contact@diaserve.de

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Applications:

MBPClone1usedtodetectmyelindensityintheopticnervebyIHC-PImagecaption:(B)IgGNMO+reducedmyelinexpression(MBPimmunoreactivity)(P < 0.01).(Marignier,Retal.)

MBPClone1usedtodetectMBPexpressioninmyelinsheathsofadultratsbyICC(Brunner,Cetal.)Dilutionused:1:3000

©BioservUK2017

BioServUKLtdTheInnovationCentre,217Portobello,Sheffield,S14DPTel:+44(0)1142242235Email:info@bioservuk.com

DiaserveLaboratoriesGmbHSeeshaupterStr.27,82393,Iffeldorf,GermanyTel:+49(0)8856803605Email:contact@diaserve.de

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MBPClone1usedtodetectmyelinexpressioninneonatalrats(braintissue)byIHC-PImagecaption:...B...HYP+NACandShamgroupsarenotappreciablydifferentinPLPorMBPstaining,indicatingpreservationofmyelinexpression...(Jatana,Metal.)Dilutionused:1:200

References:

1. Marignier,R.,Nicolle,A.,Watrin,C.,Touret,M.,Cavagna,S.,Varrin-Doyer,M.,Cavillon,G.,Rogemond,V.,Confavreux,C.,Honnorat,J.,Giraudon,P.(2010)OligodendrocytesaredamagedbyneuromyelitisopticaimmunoglobulinGviaastrocyteinjury.Brain;133(9):2578-2591.

2. Brunner,C.,Lassmann,H.,Waehneldt,T.V.,Matthieu,J-M.andLinington,C.(1989)DifferentialUltrastructuralLocalizationofMyelinBasicProtein,Myelin/OligodendroglialGlycoprotein,and2′,3′-CyclicNucleotide3′-PhosphodiesteraseintheCNSofAdultRats.JournalofNeurochemistry,52:296–304.

3. Jatana,M.,Singh,I.,Singh,A.K.,Jenkins,D.(2006)CombinationofSystemicHypothermiaandN-acetylcysteineAttenuatesHypoxic-IschemicBrainInjuryinNeonatalRats.NatureNews.NaturePublishingGroup.

4. Chujor,S.N.,Regele,H.,Bernheimer,H.,Meeker,H.C.,Levis,W.R.,Schwerer,B.(1991)Serumantibodiesagainstperipheralnervoussystemantigensinleprosy.InternationalJournalofLeprosy;59(4):590-7.WB,Dilutionused1:2,000-1:8,000

5. Groome,N.P.,Harland,J.,Dawkes,A.(1985)Preparationandpropertiesofmonoclonalantibodiestomyelinbasicproteinanditspeptides.NeurochemistryInternational,Volume7,Issue2,Pages309-317,ISSN0197-0186.ELISA,Dilutionused1:1000

6. Groome,N.P.,Dawkes,A.,Gales,M.,Hruby,S.,Alvord,E.C.Jr.(1986)Region-specificimmunoassaysforhumanmyelinbasicprotein.JournalofNeuroimmunology;12(4):253-64.ELISA