Cytology1 prelab

LAB 2 CYTOLOGY

1. Single mitochondria, RER & Lipid droplet (EM)2. Mitochondria ,RER & Secondary lysosomes (EM)3. Mitochondria, SER & glycogen granules (EM)4. T.S of centriole , Golgi complex &coated vesicle (EM)5. Golgi complex (EM)6. Peroxisome & Golgi complex (EM)7. Golgi apparatus in epididymis (LM)

Mitochondria

A. Mitochondria.Outer membrane and inner folded membrane.Mitochondrial cristae. Inter membranous space.Matrix space. B. Lipid droplet.Large round black structure.C. RER.Network of membranous tubules.Parallel stacks of flattened cisternae.Ribosomes are attached to the surface of RER.

1.A: Single mitochondria, B:Lipid droplet & C:RER.

A

B

C

A. Mitochondria. B. RER. C. Secondary lysosomes. Filled with vacuoles called residual bodies. Lysosomes are membranous cell organelles.

Contains heterogeneous materials with light and dark areas.

A

B

c

2. A:Mitochondria ,B:RER &C: Secondary lysosomes

• A. Smooth endoplasmic reticulum.• Network of branching tubules.• No ribosomes on its surface.• B. Glycogen granules.• C. Mitochondria.

3.A: SER, B:glycogen granules & C:Mitochondria.

A

B

C

Smooth endoplasmic reticulum

• Consists of tubular cisternae.• Has NO ribosomes on its

membrane.

Rough endoplasmic reticulum

• Consists of saclike, parallel stacks of flattened cisternae.

• Has ribosomes on its membrane.

4.GOLGI COMPLEX..

• 1- Flattened saccules: arranged one above the other forming a stack.

a- Immature face ( cis face) which is usually convex in shape and directed towards the nucleus.

b- Mature face (trans face) which is usually concave in shape and directed towards cell membrane or free surface.

• 2- Small vesicles (transfer vesicles).• 3- Large vesicles (secretory vesicles ).

4. Golgi complex

4.GOLGI COMPLEX..

5. T.S of centriole , Golgi complex & coated vesicle.

T.S of CENTRIOLE..

• 1.They are non membranous organelles. 2.Each centriole is cylinder in shape.• 3.In cross section, the wall of the centriole is formed of 9

bundles of microtubules and each bundle is formed of 3 microtubules (triplet).

PEROXISOME..

• Spherical membranous organelles.• Have a dense central region called crystaline core.• Fine granular homogenous content.

6. PEROXISOME & GOLGI COMPLEX.

7. Golgi apparatus in epididymus a. Dark stained area in •cells of epididymis

b. Supranuclear in position

A