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COOPERATIVE EDUCATION IN AGRICULTUREat Bayer CropScience
In PhilippinesMiss Atitaya Chumpol ID. 543030503-6
Plant Pathology1
OUTLINE
I. COMPANY ESTABLISHMENT
II. PROJECT
III. ADVANTAGE
IV. ACKNOWLEDGEMENT
2
COMPANY ESTABLISHMENT
3
COMPANY ESTABLISHMENT
•Bayer CropScience (ADSEA)
•The Masiit Trial Station
•Organization
COMPANY ESTABLISHMENT
COMPANY ESTABLISHMENT
Bayer CropScience (ADSEA):
The Agronomic Development Southeast Asia (AD-SEA) Field Research and Development Center is part of Bayer CropScience’s global AD field testing organization.
COMPANY ESTABLISHMENT
The Masiit Trial Station•8.3 hectares•70% - lowland paddy (rice) •30% - upland area (vegetable and field crops) •Strategically located near science community of Los Banos. •Serves as the main field research and development center of Bayer Crop Science in Southeast Asia. •It also serves as Training Center for APAC.
COMPANY ESTABLISHMENTOrganization
•Our team in Philippines consists of 8 Researchers, a Trial Manager and an Administrator.•In Thailand, we have a lean team of 4 in the field.•In Vietnam, we contract up to 20 trials to the local development team
PROJECTCulture media and light condition for
sporulation of Pyricularia grisea
9
PROJECT
•Introduction
•Objectives
•Materials and Methods
•Results
•Conclusion
PROJECT
INTRODUCTION
Symptom of rice blast Pyriclaria grisea
PROJECT
OBJECTIVES
To study culture media and light
condition for sporulation of Pyricularia
grisea and study morphology of
Pyricularia grisea isolates on the
difference of culture media.
PROJECT
MATERIALS AND METHODS
I. Collection and isolation
II. Culture media under light conditions for
Pyricularia grisea and study morphology of
Pyricularia grisea isolate
III. Counting spore
PROJECT
MATERIALS AND METHODS
I. Collection and isolation
Pyricularia griseaClorox 10% Rice blast symptom
2 isolates: Pia isolate and Film isolate
PROJECT
MATERIALS AND METHODS
II. Culture media under light conditions for Pyricularia griseaand study morphology of Pyricularia grisea isolate (10 days)
1. Potato Dextrose Agar (PDA)
2. Rice Dextrose Agar (RDA)
3. Rice Polish Agar (RPA)
4. Prune Juice Agar (PJA)
5. Vegetable Juice Agar (V8 agar)
Culture media
1. dark 10 days
2. dark 7 days + black light blue lamp 3 days
3. dark 7 days + yellow light 3 days
4. dark 9 days + black light blue lamp 1 day
5. dark 9 days + yellow light 1 day
Light conditions
PROJECT
MATERIALS AND METHODS
III. Counting spore
Scraped spore
Pyricularia grisae microscope
Haemacytometer
PROJECT
RESULTS OF MORPHOLOGY
0
1
2
3
4
5
6
7
8
9
Pia Film Pia Film Pia Film Pia Film Pia Film
2 4 6 8 10
PDA
RDA
RPA
PJA
V8
Figure1 . Graph of evaluate diameter mycelium of Pyricularia grisea isolates on different culture media every 2 days for 10 days.
Number of diameter (cm)
Culture media
Isolates
DatePDA = Potato Dextrose AgarRDA = Rice Dextrose AgarRPA = Rice Polish AgarPJA = Prune Juice AgarV8 = Vegetable juice Agar
PROJECT
Date IsolateNumber of diameter (cm) on media¹)
PDA RDA RPA PJA V8
2 Pia ++ ++ ++ ++ ++
Film - - - - +
4 Pia +++ +++ +++ +++ +++
Film + + + + +
6 Pia +++ +++ +++ +++ +++
Film + + + + ++
8 Pia +++ +++ +++ +++ +++
Film + + + + ++
10 Pia +++ +++ +++ +++ +++
Film + + + + ++
Table1. Evaluate densities mycelium of Pyricularia grisea isolates on different culture media every 2 days for 10 days.
Densities of mycelia+ = low++ = medium+++ = height
¹)PDA = Potato Dextrose AgarRDA = Rice Dextrose AgarRPA = Rice Polish AgarPJA = Prune Juice AgarV8 = Vegetable juice Agar
RESULTS OF MORPHOLOGY
PROJECT
PDA RDA RPA
PJA V8
Pia isolate
RESULTS OF MORPHOLOGY
PROJECT
Film isolate
PDA RDA RPA
PJA V8
RESULTS OF MORPHOLOGY
PROJECT
RESULTS OF SPORULATION
Isolates
Media¹)/light
conditions
Number of conidia (10⁴/ml)
Dark
10days
Dark 7days+
Yellow light 3
days
Dark 7days+
Black light
blue 3 days
Dark 9days+
Yellow light 1
day
Dark 9days+
Black light
blue 1 day
PDA 0 0 0 0 0
RDA 0 0 0 0 0
Pia RPA 0 0 0 0 0
PJA 0 0 0 0 0
V8 0 0 0 0 0
PDA 0 0 0 0 0
RDA 0 0 0 0 0
Film RPA 0 0 0 0 0
PJA 0 0 26.5 0.83 9.8
V8 0 6.5 24.4 7.2 16
Table2. Effect of different treatments to induce Pyricularia grisea isolates to sporulationat 10 days after incubation.
¹)PDA = Potato Dextrose AgarRDA = Rice Dextrose AgarRPA = Rice Polish AgarPJA = Prune Juice AgarV8 = Vegetable juice Agar
PROJECT
CONCLUSION
• The best culture media for mycelium was rice polish agar media of isolates, Pia isolate on rice polish agar full plates at 4 days, for mycelium and density of Pyricularia grisea depend on isolates.• The best culture media and light conditions for sporulation was prune juice agar at dark 7days+ black light blue 3 days light conditions. In
the future should study light condition for sporulation of Pia isolate.• In the future could use prune juice agar under dark 7days+ black
light blue 3 days light conditions for sporulation because no mycelium, so easy to management.
ADVANTAGE
23
ADVANTAGE
•Learning from internship
•Advantage for company
•Other activities
ADVANTAGE
Learning from internship
1. Learning about real working
2. Punctuality
3. Learning about working with other people
4. Learning about company system
5. Can apply internship experience to real working in the
future
6. Learning about planing for work
7. Responsibilities
8. Learning additional language
ADVANTAGE
Advantage for company
Culture media and light condition for sporulation of Pyricuralia grisea
ADVANTAGE
Other activities
Rice transplantingThe north of Philippines
ADVANTAGE
Other activities
Planting transplantingApply vermicompost
ADVANTAGE
Other activities
Apply fertilizer TrellisingCover soil
ACKNOWLEDGEMENT
30
ACKNOWLEDGEMENT
• The Agronomic Development Southeast Asia (AD-SEA) Field Research and Development Center(Bayer CropScience)• Mr. Leo Hawod and every body at Bayer CropScience• Faculty of agriculture and Khon kaen university • Dr. Anan Wongcharone and all my teacher • My family and my friends
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