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Body tube Rotates to move eyepiece
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Compound Light Microscope
• Has two lenses• Light must pass through object to be seen
Eyepiece
•Maginifies 10X
•Do Not Touch
•Clean with lens paper only
Body tube
Rotates to move eyepiece
Revolving Nosepiece
Turns to changes objectives
High Power Objective
Magnifies 40X
Total magnification 400X
Low Power Objective
Magnifies 4x
Total 40X
Medium Power
Magnifies 10X
Total 100X
Stage
Place slide to be viewed
Diaphragm-disc under stage
Changes the amount of light passing through specimen
Base
Place hand under base to hold or carry
Coarse Adjustment
Moves stage to focus
Never use when on High Power
Fine Adjustment
Moves stage small amount to focus
Arm
Hold to carry microscope
Rules for using the Microscope
• Never touch lenses• Use lens paper only to clean lenses
(eyepiece and objectives)• Carry with two hands, one on arm and the
other under the base• Never use coarse adjustment with high
power objective
Important Words To Know• Specimen-the object or organism you are
going to look at through the microscope• Slide-rectangle piece of glass to put your
specimen on• Cover glass-glass square you put over the
specimen• focus-to make clear• Field of view- the circle of light you see
when looking through the microscope
Making a slide
• Place specimen in center of slide• Place drop of water over specimen• Place cover slip over specimen at an angle
Microscope Procedure
• Start with low power• Focus with coarse adjustment, make final
adjustment with fine adjustment• Center object in field of view• Change to medium power• Focus and center object• Change to high power• Focus with fine adjustment only
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