CARLOS HENRIQUE MARTINS DA SILVA , SIDNEI DE SOUZA , … · CARLOS HENRIQUE MARTINS DA SILVA1,...

Implementation of computerized process and a management system at the

Leishmania Collection of the Oswaldo Cruz Institute [Coleção de Leishmania

do Instituto Oswaldo Cruz] – CLIOC

CARLOS HENRIQUE MARTINS DA SILVA1, SIDNEI DE SOUZA2, ELISA CUPOLILLO3

Laboratório de Pesquisa em Leishmaniose, Coleção de Leishmania do Instituto Oswaldo Cruz

IOC/FIOCRUZ, Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ, Brasil 2Centro de Referência em

Informação Ambiental - CRIA

CLIOC was created in 1980 with the support of the Oswaldo Cruz Institute, the Oswaldo Cruz Foundation and the World Health Organization.

CLIOC is registered with the World Federation for Culture Collections (WFCC- WDCM 731).

CLIOC is a partner of the demonstration project for a Global Biological Resource Centre Network.

The ABC of BRCs:

Authentication process for biological material

Best practice in preservation

Confirmed, validated information

From culture collection to BRC

Here we describe the computerization process adopted at CLIOC

by using a management system

CLIOC received more than 3,000 samples for deposit and/or characterization

and today has more than 1,000 strains available, representing two sections, three

subgenera and about 30 species.

Crithidia Leptomonas Herpetomonas Blastocrithidia Leishmania Trypanosoma Phytomonas Endotrypanum

Tripanossomatidae

Protozoa

Kinetoplastida

LeishmaniaViannia

Subreino

Ordem

Família

Gênero

Seções

Subgênero

Complexo

Espécie

L. donovani L. tropica L. mexicana L. major L. braziliensis L. guyanensis

L. donovani¹

L. archibaldi¹

L. infantum²

L. chagasi²

L. tropica

L. killicki

L. aetthiopica

L. major8

L. gerbilli8

L. enrietti

L. venezuelensis

L. forattinii **

L. amazonensis³

L. garnhami³

L. mexicana4

L. pifanoi4

L. aristidesi

L. braziliensis5

L. peruviana5

L. naiffiL. guyanensis6,7

L. panamensis6

L. shawi7

L. lainsoni

L. equatoriensis

L. colombiensis

L. hertigi

L. deanei

L. herrem

EuleishmaniaParaleishmania

L. arabica L. turanica L. lindenbergi L. utingensis

Sauroleishmania*

L. agami

L. adleri

L. tarentolae

L.hemidactyli

L. ceramodactyli

L. zmeevi

L. hoogstraali

L. gymnodactly

CLIOC is now fully computerized through

this system, and is able to register a wealth

of information about the samples collected,

including biological (e.g. growth curve, in

vitro sensibility to antimonial, infectivity,

virulence), biochemical (e.g. isoenzymatic

pattern, proteomic), molecular (e.g. DNA

sequences, gene expression), clinical and

ecological traits.

Services through online forms and the online

catalog are available through its web site

(http://clioc.ioc.fiocruz.br).

Stock control and strain distribution

management are also covered by

Sicol.

CLIOC is also partially

georeferenced and connected

to the speciesLink network,

aiming to help predictive

studies on Leishmania species

and genotype dispersion.

These strains came from many localities and different hosts, most from

South America.

A constant concern of this collection has been with

epidemiological data associated with the strains collected.

All deposited strains are submitted to

biochemical identification. Biological and

molecular characterizations have also been

conducted through research projects, lead

by CLIOC or by other research groups.

Supported by

Records from the Country of Origin

Brazil - 2303 Algeria - 1 Argentina - 23

Azerbaijian - 2 Belize - 6 Bolivia - 71

China - 8 Colombia - 36 Costa Rica - 35

Egypt - 1 Ecuador - 111 Ethiopia - 6

France - 3 Greece - 2 Honduras - 18

India - 5 Iraq - 2 Israel - 16

Jordan - 3 Kenia - 3 Mexico - 14

Nicaragua - 17 not informed - 18 Panama - 21

Paraguay - 37 Peru - 16 Portugal - 4

Dominican Republic - 2 Senegal - 1 Sudan - 23

Sw eden - 1 Tunísia - 3 Turkmenistan - 1

USSR - 11 Uzbekistan - 1 Venezuela - 241