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Non-duplicate Unigenes Selection Protocol. Large-scale EST assembly using phrap. Contigs. Unassembled ESTs. Unigenes. Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs. Rearray selected ESTs to 384-well plates. - PowerPoint PPT Presentation
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Bioinformatics
Lab
Non-duplicate Unigenes Selection Protocol
Large-scale EST assembly using phrap
Contigs Unassembled ESTs
Remove undesirable sequences, such as, retrotranspsons, organellar gene sequences, E. coli and phage DNA sequences and rRNAs
Rearray selected ESTs to 384-well plates
Plasmid DNA preps
5’ sequencing
3’ sequencing PCR amplification of inserts
Unigenes
Sequence processing
EST validation and duplicates removal
Probe delivery
Bioinformatics
Lab
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