Assembly and Deformation of the Bacterial Cell Wall

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Assembly and Deformation of the Bacterial Cell Wall. Rico Rojas Huang and Theriot Labs. Cell Morphogenesis – The cell wall defines cell shape. Spirochete Bacteria. Micrasterias. Phycomyces. Acetabularia. Pollen tube growth. Pulsatile pollen tube growth. - PowerPoint PPT Presentation

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Assembly and Deformation of the Bacterial Cell Wall

Rico Rojas

Huang and Theriot Labs

MicrasteriasSpirochete Bacteria

PhycomycesAcetabularia

Cell Morphogenesis – The cell wall defines cell shape

Pollen tube growth

Pulsatile pollen tube growth

Actin homologs orchestrate cell wall assembly in bacteria

Jones 2001

Research Agenda

1. What is the growth rate dependence on turgor pressure?2. What determines MreB localization?

What is the growth rate dependence on turgor pressure – varying the turgor pressure

Furchgott 2011

Growth rate dependence on turgor pressure – computational framework

MreB Localization – MreB binds ATP and mutations around the nucleotide binding pocket affect MreB localization and cell shape in Caulobacter

Dye 2011

Using MreB mutants as a perturbation to study localization

E. coliC. crescentus

WT

D189G

A325P

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