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APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
AGP MONOREAGENT 2
ND GENERATION
1. Reagent preparation Sample: Ready for use Reagent (R1): Monoreagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 250 Name AGP
R2 volume 0 Digits *
R3 volume 0 M-wave.L. 340
R4 volume 0 S-wave.L. 694
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 10 M-DET.P.l 0
Serum dil.method SPECIAL M-DET.P.m 40
Serum dil.s.vol 10 M-DET.P.n 41
Serum dil.volume 90 S-DET.P.p 0
Serum dil.posit RTT position diluent S-DET.P.r. 0
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 20 M-DET.P.m
Serum dilut.method (u) STD M-DET.P.n
Serum dil.smp.vol (u) 30 S-DET.P.p
Serum diluent vol (u) 120 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 5 * Endpoint
Serum dilut.method (d) SPECIAL Re.absorb (u) 1.700
Serum dil.smp.vol (d) 5 Re.absorb (d) -1.450
Serum diluent vol (d) 95
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 122A002 25 mL Monoreagent MPH/STH-001 Protein Standard High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
ASL (250 – 50 KIT) 1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Latex, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 100 Name ASL
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 594
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 6 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 6 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 3 117 CTT position diluent 9.999 -9.999
2 * ** Special 5 95 CTT position diluent 9.999 -9.999
3 * ** Special 10 90 CTT position diluent 9.999 -9.999
4 * ** Standard 30 120 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 129C004 1 x 10 mL Antiserum 2 x 25 mL Buffer ASL/STH-5X1 ASL Standard High, 1 mL ASL/CON-001 ASL Control, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 11.01.2007
APPLICATION NOTE ADVIA 1200
C1 ESTERASE INHIBITOR N-DIL (AUS-KIT) 1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 180 Name C1I
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 10 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 2 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 5 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Standard 30 120 CTT position diluent 9.999 -9.999
2 * ** Standard 30 120 CTT position diluent 9.999 -9.999
3 * ** Standard 30 120 CTT position diluent 9.999 -9.999
4 * ** Standard 30 120 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information C1I/AUS-000 1x 5 mL Antiserum 2x 25 mL Buffer MPS/STH-001 Protein Standard High, 1 mL MPS/STS-5X1 Protein Standard Set, 5 x 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 06.06.2006
APPLICATION NOTE ADVIA 1200
COMPLEMENT C3 BI (AUT-KIT) 1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 90 Name C3C
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 4 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 3 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Standard 30 120 CTT position diluent 9.999 -9.999
2 * ** Standard 30 120 CTT position diluent 9.999 -9.999
3 * ** Standard 30 120 CTT position diluent 9.999 -9.999
4 * ** Standard 30 120 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information C3C/AUT-000 1 x 10 mL Antiserum 5 x 25 mL Buffer MPS/STS-5X1 Protein Standard Set, 5 x 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
CRP (250 – 50 KIT) 1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 100 Name CRP
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 25 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 10 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 5 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 6
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information 117C004 1 x 10 mL Antiserum 2 x 25 mL Buffer CRP/STH-001 CRP Standard High, 1 mL CRP/COL-001 CRP Control Low, 1 mL CRP/COH-001 CRP Control High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 20.07.2010 APPLICATION TO BE VALIDATED BY THE USER
APPLICATION NOTE ADVIA 1200
CRP ULTRA SENSITIVE N-DIL (U2A–KIT) LATEX METHOD
1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 100 Name CRP
R2 volume 0 Digits *
R3 volume 24 M-wave.L. 600
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 4 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 2 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 6
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information CRP/U2A-000 1 x 10 mL Latex 2 x 25 mL Buffer CRP/SHU-001 CRP US Standard High ,1 mL CRP/COU-001 CRP Control Ultra low, 1mL CRP/COL-001 CRP Control Low , 1 mL CRP/COH-001 CRP Control High , 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
IGA MONOREAGENT 2
ND GENERATION
1. Reagent preparation Sample: Ready for use Reagent (R1): Monoreagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 250 Name IGA
R2 volume 0 Digits *
R3 volume 0 M-wave.L. 340
R4 volume 0 S-wave.L. 694
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 4 M-DET.P.l 0
Serum dil.method SPECIAL M-DET.P.m 40
Serum dil.s.vol 10 M-DET.P.n 41
Serum dil.volume 90 S-DET.P.p 0
Serum dil.posit RTT position diluent S-DET.P.r. 0
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 4 M-DET.P.m
Serum dilut.method (u) STD M-DET.P.n
Serum dil.smp.vol (u) 30 S-DET.P.p
Serum diluent vol (u) 120 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) SPECIAL Re.absorb (u) 1.700
Serum dil.smp.vol (d) 5 Re.absorb (d) -1.450
Serum diluent vol (d) 95
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 104A002 25 mL Monoreagent MPH/STH-001 Protein Standard High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
IGG MONOREAGENT 2
ND GENERATION
1. Reagent preparation Sample: Ready for use Reagent (R1): Monoreagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 250 Name IGG
R2 volume 0 Digits *
R3 volume 0 M-wave.L. 340
R4 volume 0 S-wave.L. 694
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 2 M-DET.P.l 0
Serum dil.method SPECIAL M-DET.P.m 40
Serum dil.s.vol 10 M-DET.P.n 41
Serum dil.volume 90 S-DET.P.p 0
Serum dil.posit RTT position diluent S-DET.P.r. 0
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 4 M-DET.P.m
Serum dilut.method (u) STD M-DET.P.n
Serum dil.smp.vol (u) 30 S-DET.P.p
Serum diluent vol (u) 120 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) SPECIAL Re.absorb (u) 1.700
Serum dil.smp.vol (d) 5 Re.absorb (d) -1.450
Serum diluent vol (d) 95
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 105A002 25 mL Monoreagent MPH/STH-001 Protein Standard High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 27.11.2012
APPLICATION NOTE ADVIA 1200
IgM KIT 2nd
Generation 1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 150 Name IgM
R2 volume 0 Digits *
R3 volume 30 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 5 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 2 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Standard 30 120 CTT position diluent 9.999 -9.999
2 * ** Standard 30 120 CTT position diluent 9.999 -9.999
3 * ** Standard 30 120 CTT position diluent 9.999 -9.999
4 * ** Standard 30 120 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information 106C002 1 x 10 mL Antiserum
5 x 25 mL Buffer MPS/STS-5X1 Protein Standard Set, 5 x 1 mL 139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
IGM MONOREAGENT 2
ND GENERATION
1. Reagent preparation Sample: Ready for use Reagent (R1): Monoreagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 250 Name IGM
R2 volume 0 Digits *
R3 volume 0 M-wave.L. 340
R4 volume 0 S-wave.L. 694
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 20 M-DET.P.l 0
Serum dil.method SPECIAL M-DET.P.m 40
Serum dil.s.vol 10 M-DET.P.n 41
Serum dil.volume 90 S-DET.P.p 0
Serum dil.posit RTT position diluent S-DET.P.r. 0
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 20 M-DET.P.m
Serum dilut.method (u) STD M-DET.P.n
Serum dil.smp.vol (u) 30 S-DET.P.p
Serum diluent vol (u) 120 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 10 * Endpoint
Serum dilut.method (d) SPECIAL Re.absorb (u) 1.700
Serum dil.smp.vol (d) 5 Re.absorb (d) -1.450
Serum diluent vol (d) 95
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 106A002 25 mL Monoreagent MPH/STH-001 Protein Standard High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
MICROALBUMIN (250 – 50 KIT) 1. Reagent preparation Sample: Centrifuged urine, ready for use Reagent: Buffer, ready for use Start: Antiserum, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 100 Name MAL
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 10 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 4 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 2 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 6
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information 102C004 1 x 10 mL Antiserum 2 x 25 mL Buffer MAL/STD-001 Microalbumin standard, 1 mL MAL/CON-001 Microalbumin control, 1 mL MAL/CON-005 Microalbumin control, 5 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
RF 3RD
GENERATION Hu Agg IgG Method
1. Reagent preparation Sample: Ready for use Reagent: Buffer, ready for use Start: Reagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 100 Name RF
R2 volume 0 Digits *
R3 volume 20 M-wave.L. 340
R4 volume 0 S-wave.L.
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 30 M-DET.P.l 0
Serum dil.method A dilution 40
Serum dil.s.vol 30 41
Serum dil.volume 120 17
Serum dil.posit RTT position diluent 18
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 15 M-DET.P.m
Serum dilut.method (u) NONE M-DET.P.n
Serum dil.smp.vol (u) 0 S-DET.P.p
Serum diluent vol (u) 0 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 10 * Endpoint
Serum dilut.method (d) STD Re.absorb (u) 1.700
Serum dil.smp.vol (d) 30 Re.absorb (d) -1.450
Serum diluent vol (d) 120
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 6
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5 * ** Standard 30 120 CTT position diluent 9.999 -9.999
* Data entered by operator ** Concentration of standard
3. Order information 100C011 1 x 10 mL Reagent 2 x 25 mL Buffer RHF/SST-001 RF Standard Super High, 1 mL RHF/CON-001 RF Control, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
APTEC DIAGNOSTICS 19.02.2008
APPLICATION NOTE ADVIA 1200
TRF MONOREAGENT 2
ND GENERATION
1. Reagent preparation Sample: Ready for use Reagent (R1): Monoreagent, ready for use Calibrator: Ready for use. Use saline 9 g/L as zero point.
2. Analytical Parameters (Chemistry) TYPE #
Analytical conditions Sub-analy. conditions
R1 volume 250 Name TRF
R2 volume 0 Digits *
R3 volume 0 M-wave.L. 340
R4 volume 0 S-wave.L. 694
R1 diluent vol 0 Analy.Mthd EPA
R2 diluent vol 0 Calc.Mthd MSTD
R3 diluent vol 0 Qualit.judge Not do
R4 diluent vol 0 Calculation method setting
Serum reac.s.vol 7 M-DET.P.l 0
Serum dil.method SPECIAL M-DET.P.m 40
Serum dil.s.vol 10 M-DET.P.n 41
Serum dil.volume 90 S-DET.P.p 0
Serum dil.posit RTT position diluent S-DET.P.r. 0
Reaction time 10 min. Check D.P.I. 0
Reagent 1 stir Weak Limit value 0.003
Reagent 2 stir Weak Variance 10.0
Reagent 3 stir Weak * Prozone
Reagent 4 stir Weak Prozone form NONE
Prozone limit
Reanalysis conditions Prozone judge
Serum reac.smp.vol (u) 7 M-DET.P.m
Serum dilut.method (u) STD M-DET.P.n
Serum dil.smp.vol (u) 30 S-DET.P.p
Serum diluent vol (u) 120 S-DET.P.p
Serum diluent posi (u) 0 S-DET.r
Serum reac.smp.vol (d) 7 * Endpoint
Serum dilut.method (d) SPECIAL Re.absorb (u) 1.700
Serum dil.smp.vol (d) 5 Re.absorb (d) -1.450
Serum diluent vol (d) 95
Serum diluent posi (d) 0
Standard settings Multi-STD
Formula LOGIT– LOG3 Axis conv. NO CONVERT Points 5
POS Coeff (FV) Dil.method Dil.smp.vol Diluent vol. Diluent pos. STD-H STD-L
BLK *
1 * ** Special 2 148 CTT position diluent 9.999 -9.999
2 * ** Special 3 117 CTT position diluent 9.999 -9.999
3 * ** Special 5 95 CTT position diluent 9.999 -9.999
4 * ** Special 10 90 CTT position diluent 9.999 -9.999
5
* Data entered by operator ** Concentration of standard
3. Order information 109A002 25 mL Monoreagent MPH/STH-001 Protein Standard High, 1 mL
139F003 Immunology Control Low, 1mL 139F002 Immunology Control High, 1 mL
Recommended