Aedes aegypti control programmes in Brazil · 2017-05-23 · Aedes aegypti control programmes in...

Aedes aegypti control programmes in Brazil

Margareth L. Capurro mcapurro@icb.usp.br

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 6, June 2006

Integrate Control for Aedes aegypti Population Supression

Integrate Control for Aedes aegypti Population Supression

Integrate Control for Aedes aegypti Population Supression

Integrate Control for Aedes aegypti Population Supression

80%

Integrate Control for Aedes aegypti Population Supression

80%

80%

Integrate Control for Aedes aegypti Population Supression

80%

80%

Step 2. Replacement

Natural Population

Step 1. Suppression

Open Field Release of OX513A Aedes aegypti Transgenic line evaluation

tTA Minimal

promoter tetO

Antidote (Tetra)

death

Thomas et al. 2000 Science 287: 2474-6

Repressive of Insects carrying a Dominant Lethal gene (RIDL)

X

Males for releases 1,5 million/week

Project Phase 2 – Jacobina - Bahia

Pupa Transportation

Pupa transportation (LEMI)

C25 BOD 16oC ON

Preparation for release Arriving at LEMI Emergency , Monitoring and Information Lab

180,000 per container

First Trial

Second Trial

•We need Genetic Sexing Strain (GSS)

•Producing Sterile male strain (no Larvae)

•Use of tetracycline only in colonies

What do we learn?

Sterility Conditional Construct - SCC

♀

♂ Antidote

(Tet +) Viable Eggs

♀

♂ No

Antidote No Viable Eggs

X

SCC Transgenes

DsRed SV40 tTAV ttO Michelob_X 3XP3 B2tubulin

IAP Antagonist

Two Effector molecules:

DsRed SV40 tTAV ttO Endonuclease 3XP3 B2tubulin

Endonuclease

Transgenic Lines

150 pb 350 pb 150 pb

60% Homozygous 80% Reduction

Sterile Conditional Construct (SCC)

Improving transgenic lines Aedes aegypti and Aedes albopictus

• Producing Sterile male strain (no Larvae) ✔

• Producing GSS (Genetic Sexing Strain)

Dsx knockout

Step 2. Replacement

Natural Population

Step 1. Suppression

Gene Introduction Virus-regulated mosquito gene Suicidal Model (Double death model) Natural Population

Gene Introduction Virus-regulated mosquito gene Suicidal Model (Double death model) Natural Population

+ Introduction

Gene Introduction Virus-regulated mosquito gene Suicidal Model (Double death model)

Virus Infection

Natural Population +

Introduction

Gene Introduction Virus-regulated mosquito gene Suicidal Model (Double death model)

Virus Infection

Natural Population +

Introduction

HS-PRO

NS3 Cleavage site

RRRRSAG

Gene Introduction Virus-regulated mosquito gene Suicidal Model (Double death model)

Lúmen do RER C-terminal

L2L4

L6L8

Sec61γSec61β

Lúmen do RER C-terminal

L2L4

L6L8

Sec61γSec61β

Sec61γ

ER localization

Mx

Lethal protein Constitutive Promoter

Step 2. Replacement

HS EGFP Mx Sec61

RRRRSAGRRRRSAG

A5

Cleavage sites

1 ER Lumen

Cytoplasm SA

G

RR

RR

2

3

C

pr

M

2A

NS3 4A 4B NS5

E

NS1

2B

4

6 X or

APOPTOSIS

5

• Double death model - Infection phenotypes in dengue challenged mosquito?

PIE – EGFP

GFP filter

DAPI Merge

Light

Sec61 – Mx – EGFP

GFP filter

DAPI Merge

Light

ER marker red dye

GFP filter Light

Merge

Sec61 – Mx – EGFP

• C6/36 cells - Transfection and D2 challenge:

3 days after DEN2

Sec61 – Mx – EGFP

DAPI

GFP filter Light

Merge

Intrathoracic injection - Survivorship

0 2 4 6 8 10 12 146065707580859095

100

DUI-Y CS (n=81)DUI-Y IS (n=66)p=0.026

Days after injectionPe

rcen

t sur

viva

l0 2 4 6 8 10 12 14

6065707580859095

100

Wild type CS (n=77)Wild type IS (n=82)

p=0.389

Days after injection

Perc

ent s

urvi

val

Survival rates of wild type (Higgs-strain) and DUI-Y transgenic line challenged with DENV-2 via intrathoracic injection. Five-to-seven day old females were injected with control supernatant (CS) and DENV-2 infected supernatant (IS) (vial load = 7.0 x 106 genomic copies). Females were recovered during 24hs after injection and survived females were maintained on 10% sucrose solution and censored during 14 days after injection (14DPI). Solid lines are showing survival curves of females injected with CS and dotted lines are showing survival curves of females injected with IS. Survival curves were statistically analyzed by Log-rank (Mantel-Cox) test.

Bringing new technology to the field

Technical

Regulatory Community

Male

Nature

Wild

Sterile

Males Female

X

How the Sterility works? How the Sterile Mosquito Works? Why releasing male mosquitoes you kill mosquitoes?

Before releasing mosquitoes

• Site selection • Public Engagement:

• Evolving Public Authorities (Government and agencies);

• Local ones (community engagement); • Local people - explain what we are going to

do in that area.

Community Engagement

Total people 17,101,269 in Brazil – Based on the Brazilian Institute of Public Opinion and Statistics (IBOPE) data

Community Engagement

Talks and Lectures

City Hall Public Hearing

Leaflet distribution

Mosquito Aedes \Dengue Muriçoca (Culex)

Pica durante o dia (bite during the day) Pica durante a noite (bite during the night)

Nectar

Bar – Blood for Sale!

Only females (girls) bite

Font

e: G

oogl

e

ACTION TARGET

POPULATION LEVEL #

EVENTS #

PEOPLE Presentations/Lectures Local/Regional 10 962 Leaflets (¹) Local - 10,000 Jingle (¹) Local - -

Meetings National/ International

39 6,020

Interviews (radio) Regional 15 1,500 Interviews (TV) Regional/National 09 17,094,000 (2) Interviews (newspaper/magazine)

Local/Regional/ National

13 -

Internet (website / social network)

Regional/National 24 -(³)

Houses visited/interviewed with residents

Local 581 2,341

Meetings with local leaders, health agents

Local 16 820

Presentations at elementary and middle school

Local 08 452

Presentation at community center/city hall/others

Local 06 456

Driving truck with loudspeakers in the releasing area

Local - 500

Spots, jingles and short messages broadcasted in local radio station

Local 52 1,200 (4)

TOTAL 17,101,269

STRATEGIES

Mandatory Recommended Suggested

- Visit/interview sample/every house in the target area

- Lectures at community centers/churches – targeting adults

- Action within a local event (parade, carnival, street fairs)

- Meetings with local leaders, school principals, district managers

- Radio spots, jingles and messages broadcasted

- Driving truck with loudspeakers in the targeting area – jingle and messages

- Lectures at schools – targeting kids/teens

- Press releases by Moscamed journalists

- Use of social media: Facebook and twitter

- Press coverage at local/regional level of PAT activities: production, releases

- PAT technical personnel interviewed by local/regional/(inter)national radio stations

- Press coverage at international level of PAT activities: releases

- Press coverage at national level of PAT activities: production, releases

Spot and Jingle

SPOT

To control dengue Moscamed is releasing in this community

A large amount of TRANSGENIC MOSQUITOES .

We would like to recall that this mosquitoes are not the well known CULEX

They are transgenic MALES and they DON’T BITE.

They are good fellows that will give you protection against dengue.

For more information call a health agent or get in touch with

MOSCAMED

By the phone

(74) 3612-5399

PAT –AEDES TRANSGENIC PROJECT

This one makes the difference.

Jingle Transgenic Aedes

Universidade de São Paulo

Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular

Dr Jeff Powell

Dr Lincoln Suesdek

Dr Peter Piermarini

Dr Chun-Hong Chen

LEMI UPAT