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Advances in Hydrocarbon Remediation Technology
and Water Treatment
Ben Lee, Refine Innovations, LLC
Advances in Hydrocarbon RemediationTechnology and Water Treatment
Benjamin Lee -- Refine Innovations, LLC
Common Remediation Methods
• Contaminant Removal: “Dig and dump”• Mixture: “Solution by Dilution”• Flash off: “Land-farming”• Thermal/Incineration• Bioremediation -- Bacillus
© 2015 Refine Innovations, LLC Proprietary and Confidential
Classical Bioremediation: Bacillus© 2015 Refine Innovations, LLC Proprietary and Confidential
Bacillus MicrobeSpore
Two general bioremediation categories:• Bio-stimulation• Bio-augmentation
© 2015 Refine Innovations, LLC Proprietary and Confidential
Typical Bacillus Performance
Classical Bioremediation: Bacillus
© 2015 Refine Innovations, LLC Proprietary and Confidential
Pseudomonas Culture
Advanced Bioremediation: Pseudomonas
© 2015 Refine Innovations, LLC Proprietary and Confidential
Typical Pseudomonas Performance
Advanced Bioremediation: Pseudomonas
Pseudomonas Treatment
Pseudomonas Treatment is a Bio-augmentation process and involves 3 components:
• Biological (Culture, transport, and apply Pseudomonas Microbes at high concentrations)
• Chemical (Bio-surfactant)• Mechanical (Application equipment that ensure
even and complete contact)
© 2015 Refine Innovations, LLC Proprietary and Confidential
Chemistry© 2015 Refine Innovations, LLC Proprietary and Confidential
Bio–Surfactant• Allows microbes to move more easily through the
contaminated site• Specialized chemistry does not harm pseudomonas• Highly biodegradable (90% degraded in 8 days)
Mechanical© 2015 Refine Innovations, LLC Proprietary and Confidential
Prepped Mixture Tanks
Earth Cleaning Machine
Treated Soil Conveyor
Microbial Mixing Trailer
Application equipment ensures successfulremediation in a one-time application:• Calibrated Earth Cleaning Machine to ensure
the pseudomonas microbes are appliedevenly and effectively to all contaminatedsoil.
© 2015 Refine Innovations, LLC Proprietary and Confidential
Earth Cleaning Machine
Treated Material© 2015 Refine Innovations, LLC Proprietary and Confidential
Treated Material
Case Study: Soil Application 1
This graph shows results for a soil application performed recently in the southwest U.S. This data was obtained by an independent party, taking samples on a site treated during remediation operations on a contaminated site in North America.
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Soil Application 2
This graph shows results for a soil application, broken out by hydrocarbon chain length. This data was obtained by an independent party, taking samples on a site treated during remediation operations on a contaminated site in North America.
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Soil Application 3© 2015 Refine Innovations, LLC Proprietary and Confidential
This graph shows results for a sludge application conducted in Texas during Summer, 2015. Data is broken out by hydrocarbon chain length. This data was obtained by an independent party, taking samples on a site treated during remediation operations on oilfield sludge, tank bottoms, and drilling cuttings.
Week 1 Week 5 Week 9TPH 139500 17421 120.12C6-C12 7200 3551 24.5C12-C28 121000 12870 92.1C28-C35 11300 1000 3.52
0
20000
40000
60000
80000
100000
120000
140000
160000
ppm
Sludge Treatment - Texas, Summer 2015
Case Study: Frac Pond, South Texas
Before
After
© 2015 Refine Innovations, LLC Proprietary and Confidential
Background: Client had a Hydrocarbon (TPH and BTEX) Contaminated Frac Pond in South Texas. Pond was treated with pseudomonas: TPH and BTEX tested out at non-detect 10 days later. This project saved the client 80% over their next closest remediation option.
Case Study: Freshwater Application
This table shows the before and after contamination levels for a contaminated freshwater lake. This data was also independently obtained on a site treated in North America. Our product also works in salt water environments.
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application© 2015 Refine Innovations, LLC Proprietary and Confidential
Pre Treatment Post Treatment
Case Study: Freshwater Application© 2015 Refine Innovations, LLC Proprietary and Confidential
Pre Treatment Post treatment Water and soil are now treated, allowing natural state to return.
Case Study: Freshwater Application© 2015 Refine Innovations, LLC Proprietary and Confidential
Pre Treatment Post Treatment
Case Study: Freshwater Application© 2015 Refine Innovations, LLC Proprietary and Confidential
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
© 2015 Refine Innovations, LLC Proprietary and Confidential
Case Study: Freshwater Application
• Limonene• Citronellal• Chlorinated Solvents• Aliphatic Hydrocarbons• Creosote• Amines (DEA/etc…)
• Dichlorobenzene• Dichlorotoluene• Methyl Ethyl Ketone• Methylene Chloride• Naphthalene• Fluorine• Benzene• Toluene• Ethylbenzene• Xylene• Crude oils / LCM• Petroleum• Isoprenoids
Successful at Degrading:© 2015 Refine Innovations, LLC Proprietary and Confidential
FEATURES: • All components are TSCA, and EPA NCP approved
• Proven biological system that degrades organics to carbon dioxide and water • Readily biodegradable, water based formula • Pathogen free • DNA tested • May be shipped non-regulated world wide by any means
BENEFITS: • Total hydrocarbon degradation • Eliminate costly transportation and landfill charges, especially in remote sites • Reduce liability: removal of dozens of trucks off the roads vs “dig and dump”
remediation.• Eliminate future potential liabilities from landfill containment failure • Significant dollars savings per project • Success achieved within days/months rather than years
Overview of Technology© 2015 Refine Innovations, LLC Proprietary and Confidential
Oil Spills• Land• Water
Ground WaterBrownfields (Retail and Bulk Fuel Stations)
• Underground storage tanksAbandoned PropertyOil and Gas Industry
• Drill Cuttings• Soil• Sumps• Tank Bottoms• LCM• Flow-back and Produced Water Treatment
Applications© 2015 Refine Innovations, LLC Proprietary and Confidential
Contact
Benjamin Lee: Ben@refine-innovations.com
Phone: 866-477-1510
16301 Interstate 27, Suite BCanyon, TX 79015
www.refine-innovations.com
© 2015 Refine Innovations, LLC Proprietary and Confidential
Conference participants are eligible for up to 13 contact hours 1.3 CEUs. Forms will be available after lunch on Wednesday at
the registration are.
Return completed forms at the conclusion of the conference.
Please remember to fill out the conference survey. This will be sent to you by email.
https://www.surveymonkey.com/r/2015OGENV
Advances in Hydrocarbon Remediation Technology
and Water Treatment
Ben Lee, Refine Innovations, LLC
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