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Supplementary Figure 3: Measurement of thermodynamic stability (Tm) and hence the affinity between PGC-1α 220 and PPARγ. (A and B) Representative thermal denaturation curves using Far-UV CD spectra at pH 7.5 and pH 6.0. (C) Tabulated triplicate Tm values, average values and the standard deviation of the binary complex at pH 7.5 and pH 6.0. The differences are not statistically significant. Thermodynamic measurement using CD is performed according to [35] except the wavelength used is 230 nm.
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