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Maqsood A. Chotani, PhD (Jan 2019)
MAQSOOD AHMED CHOTANI, PhD, FCVS (APS) Associate Professor
Molecular Signaling Laboratory Center for Bioequivalence Studies & Clinical Research
Dr. Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences
University of Karachi, Karachi-75270, Pakistan maqsood.chotani@gmail.com maqsood.chotani@iccs.edu
Office (UAN): (+92-21) 111-222-292 (dial 353, then 513) Cell: +92-301-8208439
General Area of Expertise and Research Activity
Molecular & Cell Physiology Sub-field: Vascular Biology/Cell-Signaling/G Protein Coupled Receptors (GPCRs) Keywords: thermoregulation, cell signaling, G Protein Coupled Receptor, α2C-adrenoceptors,
microcirculation, vascular smooth muscle, Rap1A, in-silico modeling
Video link: “Your Cardiovascular Health: How Stress Impacts Your Tiny Blood Vessels”
www.youtube.com/watch?v=lMr5MSbZfqE
Pertinent experience
Fifteen years of academic research experience focusing on cell and molecular physiology of microvascular α2C-adrenoceptors, particularly mechanisms of gene expression and trafficking
Ability
Research, teaching and training, and administrative service experience in academic setting. Ability to run and manage an independent research laboratory, including project design, collaborations,
experimentation, submission and publication in manuscript form (as senior author), submission of research grants to federal and private agencies (as principal investigator), recruiting and supervising personnel and trainees.
Publication Metrics Cumulative impact factor: 137.0 (30 peer-reviewed publications) Total citations: 1,581 [includes 1,542 (for published manuscripts) plus 39 (for US Patent)];
(source: Google Scholar) H-index: 22 (source: Google Scholar; includes citations in journals, books,
dissertations, and patent)
Output (publications)
Journal impact factor 1 - 4.99 5 - 6.99 7 - 9.99 10
No. published 22 2 1 4
% 75.86 6.89 3.44 13.79
Maqsood A. Chotani, PhD
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Memberships in Professional Organizations
a. American Heart Association (Premium Professional Member - Silver Heart; Council on Basic Cardiovascular Sciences and Council on Peripheral Vascular Disease; 1998)
b. American Society for Pharmacology and Experimental Therapeutics (elected 2000, regular member)
c. American Society for Biochemistry and Molecular Biology (elected 2001, regular member)
d. American Physiological Society (elected 2010, regular member); Fellow of the Cardiovascular Section, elected April 23, 2018
e. The Asian Society for Vascular Biology (member, 2017)
Dissertation/Thesis
a. Chotani, M.A. 1997. Cellular and molecular analysis of regulatory mechanisms of human Fibroblast Growth Factor 1 gene expression. Dissertation. The Ohio State University, Columbus, Ohio USA
b. Chotani, M.A. 1988. Use of ordered deletions in place of shotgun cloning, and dideoxy sequencing of the mouse VL30 gene cloned in the pUC119 plasmid vector. Undergraduate Senior Independent Thesis. The College of Wooster, Wooster, Ohio USA
Honors
Sigma Xi and Phi Beta Kappa, 1988 Honors, Senior Independent Study Thesis, 1988, The College of Wooster, Wooster, Ohio, USA Department honors (Biology), 1988, The College of Wooster, Wooster, Ohio, USA Horace N. Mateer Prize in Biology, 1988, The College of Wooster, Wooster, Ohio, USA Awardee, May 10, 1994, The 20th annual ICSABER Society Graduate Student Forum, The Ohio State
University, Columbus, Ohio, USA Awardee, April 4, 2002, The First Annual Integrated OSU Medical Center Graduate & Postgraduate
Research Day, The Ohio State University, Columbus, Ohio, USA Awardee, Young Scientist Travel Award for the joint American Society for Biochemistry & Molecular
Biology/American Society for Pharmacology & Experimental Therapeutics meeting in Boston (June 4-June 8, 2000)
Awardee, Young Scientist Travel Award for the 10th International Symposium on Vascular Neuroeffector Mechanisms, Granlibakken, Lake Tahoe, California (July 12-15, 2002)
Gold Medal & cash award, Pakistan Academy of Sciences, recognizing scientific contributions in the field of Medical Sciences (2004)
Fellow of the Cardiovascular Section, American Physiological Society (April 23, 2018)
Primary & Secondary Education Karachi Grammar School, Karachi, Pakistan Government Adamjee Science College, Karachi, Pakistan
Maqsood A. Chotani, PhD
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Professional Preparation
BA Biology, minor Chemistry (1988), The College of Wooster, Wooster, Ohio, USA
PhD Molecular, Cellular, & Developmental Biology (1997), The Ohio State University, Columbus, Ohio, USA
Postdoc Molecular & Cell Biology (1997-1998) The Ohio State University, Department of Internal Medicine, Columbus, Ohio, USA
Postdoc Vascular Biology/Molecular Physiology (1998-2002), Davis Heart & Lung Research Institute, The Ohio State University, Columbus, Ohio, USA
Appointments
2002-2008 Research Scientist, Davis Heart & Lung Research Institute, The Ohio State University, Columbus, Ohio, USA
2008-2013 Principal Investigator, Center for Cardiovascular & Pulmonary Research, Research Institute at Nationwide Children’s Hospital, Columbus, Ohio, USA
2009-2013 Assistant Professor, Department of Pediatrics, The Ohio State University Medical Center, Columbus, Ohio, USA
2009-2013 Graduate faculty member, Biomedical Sciences Graduate Program (BSGP), The Ohio State University Medical Center and the Interdisciplinary Molecular, Cellular, & Developmental Biology (MCDB) Graduate Program, The Ohio State University, Columbus, Ohio, USA
2013-2015 Freelancer/Seeking new opportunities 2015-2016 Foreign Faculty/Visiting Professor Dr. Panjwani Center for Molecular Medicine & Drug Research International Center for Chemical & Biological Sciences University of Karachi, Pakistan 2016-Present Associate Professor
Molecular Signaling Laboratory Center for Bioequivalence Studies & Clinical Research (CBSCR-Lab 111) Dr. Panjwani Center for Molecular Medicine & Drug Research
International Center for Chemical & Biological Sciences University of Karachi, Pakistan Peer-Reviewed Original Articles
*Corresponding author † Shared first authorship #Senior author IF: Impact Factor (2016, or as indicated. Source: ISI Web of Knowledge, Journal Citation Reports or
ResearchGate)
1) Hodgson CP, Fisk RZ, Arora P, Chotani M. Nucleotide sequence of mouse virus-like (VL-30) retrotransposon BVL-1. Nucleic Acids Res (Oxford University Press, ISSN: 0305-1048). 18(3): 673; 1990 (PMID 2155410). IF: 10.162; Citations: 33
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2) Piletz JE, Sarasua M, Chotani M, Saran A, Halaris A. Relationship between membrane fluidity and adrenoceptor binding in depression. Psychiatry Res (Netherlands, Elsevier Science, ISSN: 0165-1781). 38: 1-12; 1991 (PMID 1682966). IF: 2.528; Citations: 12
3) Piletz JE, Sarasua M, Whitehouse P, Chotani M. Intracellular membranes are more fluid in platelets of Alzheimer's disease patients. Neurobiol of Aging (Netherlands, Elsevier Science, ISSN: 0197-4580). 12: 401-406; 1991 (PMID 1770973). IF: 5.117; Citations: 41
4) Myers RL, Payson RA, Chotani MA, Deaven LL, Chiu I-M. Gene structure and differential expression of acidic fibroblast growth factor mRNA: identification and distribution of four different transcripts. Oncogene (Nature Publishing Group, ISSN: 0950-9232). 8: 341-349; 1993 (PMID 7678925). IF: 7.519; Citations: 74
5) Payson RA, Canatan H, Chotani MA, Wang W-P, Harris SE, Myers RL, Chiu I-M. Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA. Nucleic Acids Res (Oxford University Press, ISSN: 0305-1048). 21(3): 489-495; 1993 (PMID 7680120). IF: 10.162; Citations: 57
6) Myers RL, Ray SK, Eldridge R, Chotani MA, Chiu I-M. Functional characterization of the brain-specific FGF-1 promoter, FGF-1.B. J Biol Chem (American Society for Biochemistry and Molecular Biology, ISSN: 0021-9258). 270 (14): 8257-8266; 1995 (PMID 7713933). IF: 4.125; Citations: 51
7) Chotani MA, Payson RA, Winkles JA, Chiu I-M. Human fibroblast growth factor 1 gene expression in vascular smooth muscle cells is modulated via an alternate promoter in response to serum and phorbol ester. Nucleic Acids Res (Oxford University Press, ISSN: 0305-1048). 23(3): 434-441; 1995 (PMID 7533902). IF: 10.162; Citations: 36
8) Chotani MA, Chiu I-M. A recombinant PCR approach requiring only three non-chimeric primers to generate a minigene of interest. Genetic Analysis: Biomol Eng (Netherlands, Elsevier Science, ISSN: 1050-3862). 12(3-4): 133-135; 1996 (PMID 8673737). IF: 2.94 (2001; relaunched as Biomolecular Engineering); Citations: 2
9) Chotani MA, Chiu I-M. Differential regulation of human fibroblast growth factor 1 transcripts provides a distinct mechanism of cell-specific growth factor expression. Cell Growth Differ (American Association for Cancer Research, HighWire Press, ISSN: 1044-9523). 8(9): 999-1013; 1997. Erratum in 8 (11): 1241; 1997 (PMID 9300182). IF: 3.826 (2004; relaunched as Molecular Cancer Research); Citations: 36
10) Payson RA, Chotani MA, Chiu I-M. Regulation of a promoter of the fibroblast growth factor 1 gene in prostate and breast cancer cells. J Steroid Biochem Molec Biol (Netherlands, Elsevier Science, ISSN: 0960-0760). 66(3): 93-103; 1998 (PMID 9719443). IF: 4.561; Citations: 41
11) Chotani MA, Touhalisky K, Chiu I-M. The small GTPases Ras, Rac1 and Cdc42 transcriptionally regulate expression of human fibroblast growth factor 1. J Biol Chem (American Society for Biochemistry and Molecular Biology, ISSN: 0021-9258). 275(39): 30432-30438; 2000 (PMID 10849427). IF: 4.125; Citations: 27
12) Chotani MA, Flavahan S, Mitra S, Daunt D, Flavahan NA. Silent 2C-adrenergic receptors enable cold-induced vasoconstriction in cutaneous arteries. Am J Physiol. Heart Circ Physiol (American Physiological Society, ISSN: 0363-6135). 278:H1075-H1083; 2000 (PMID 10749700). IF: 3.324; Citations: 263
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13) Su B, Mitra S, Gregg H, Flavahan S, Chotani MA, Goldschmidt-Clermont PJ, Clark KR, Flavahan NA. Redox regulation of vascular smooth muscle cell differentiation. Circ Res (American Heart Association, ISSN: 0009-7330). 89:39-46; 2001 (PMID 11440976). IF: 11.551; Citations: 165
14) Jeyaraj SC, Chotani MA, Mitra S, Gregg HE, Flavahan NA, Morrison KJ. Cooling evokes redistribution of 2C-adrenoceptors from Golgi to plasma membrane in transfected human embryonic kidney 293 cells. Mol Pharmacol (American Society for Pharmacology and Experimental Therapeutics, ISSN:0026-895X ). (Accelerated Communication) 60:1195-1200; 2001 (PMID 11723226). IF: 3.931; Citations: 124
15) *Chotani MA, Mitra S, Su BY, Flavahan S, Eid AH, Clark KR, Montague C, Paris H, Handy D E, Flavahan NA. Regulation of 2-adrenoceptors in human vascular smooth muscle cells. Am J Physiol. Heart Circ. Physiol (American Physiological Society, ISSN: 0363-6135). [special “Translational Physiology” series article] 286: H59-H67; 2004 (PMID 12946937). IF: 3.324; Citations: 87
16) *Chotani MA, Mitra S, Eid AH, Han SA, Flavahan NA. Distinct cyclic AMP signaling pathways differentially regulate 2C-adrenoceptor expression: role in serum induction in human arteriolar smooth muscle cells. Am J Physiol. Heart Circ Physiol (American Physiological Society, ISSN: 0363-6135). [special “Translational Physiology” series article] 288: H69-H76; 2005 (PMID 15345481). IF: 3.324; Citations: 33
17) Eid AH, Maiti K, Mitra S, Chotani MA, Flavahan S, Bailey SR, Thompson-Torgerson CS, Flavahan NA. Estrogen increases smooth muscle expression of α2C-adrenoceptors and cold-induced constriction of cutaneous arteries. Am J Physiol. Heart Circ Physiol (American Physiological Society, ISSN: 0363-6135). 293: H1955-H1961; 2007 (PMID 17644575). IF: 3.324; Citations: 71
18) Bayoumi MM, Alkheraije KA, El-Sayed O, Wisel S, Sarker K, Chotani MA, Zweier JL, Nuovo G, Goldschmidt-Clermont PJ, Hassanain H. Vascular hypertrophy and hypertension caused by transgenic overexpression of profilin 1. J Biol Chem (American Society for Biochemistry and Molecular Biology, ISSN: 0021-9258). 282:37632-37639; 2007 (PMID 17942408). IF: 4.125; Citations: 43
19) Eid AH†, Chotani MA†, Mitra S, Miller TJ, Flavahan NA. Cyclic AMP acts through Rap1 and JNK signaling to increase expression of cutaneous smooth muscle α2C-adrenoceptors. Am J Physiol. Heart Circ Physiol (American Physiological Society, ISSN: 0363-6135). 295: H266-H272; 2008 (PMID 18487435). IF: 3.324; Citations: 28
20) Mihai C, Chotani M, Elton TS, Agarwal G. Mapping of DDR1 distribution and oligomerization on the cell surface by FRET microscopy. J Mol Biol (Netherlands, Elsevier Science, ISSN:0022-2836). 385: 432- 445; 2009. Corrigendum in 425 (10): 1846; 2013 (PMID 19007791). IF: 4.517; Citations: 69
21) Jeyaraj SC, Unger NT, Eid AH, Mitra S, El-Dahdah NP, Quilliam LA, Flavahan NA, *#Chotani MA. Cyclic AMP-Rap1A Signaling Activates RhoA to Induce α2C-Adrenoceptor Translocation to the Cell Surface of Microvascular Smooth Muscle Cells. Am J Physiol. Cell Physiol (American Physiological Society, ISSN: 0363-6143). 303: C499-C511; 2012 (PMID 22621783). IF: 3.395; Citations: 36
This article is accompanied by an editorial by Martin C. Michel and Paul A. Insel: Michel MC, Insel PA. Can you blame cold feet on Epac (and Rap1A)? Focus on “Cyclic AMP-Rap1A signaling activates Rho to induce α2C-Adrenoceptor Translocation to the Cell Surface of Microvascular Smooth Muscle Cells.” Am J Physiol Cell Physiol. 303: C488-C489; 2012.
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This article was included in “Editor’s Picks” for summer 2012, and was highlighted in a press release by the American Physiological Society on July 31, 2012 and HealthDay news (http://consumer.healthday.com/Article.asp?AID=667527) (which was picked up by MSN (icy feet, icy reception video), US News & World Report, Raynaud’s Association, TV channels, and Popular Science).
22) Motawea HK†, Jeyaraj SC†, Eid AH, Mitra S, Unger NT, Ahmed AA, Flavahan NA, *#Chotani MA. Cyclic AMP-Rap1A Signaling Mediates Cell Surface Translocation of Microvascular Smooth Muscle α2C-adrenoceptors Through the Actin Binding Protein Filamin-2. Am J Physiol. Cell Physiol (American Physiological Society, ISSN: 0363-6143). 305: C829-C845; 2013 (PMID 23864608). IF: 3.395; Citations: 19
This is a follow-up paper to manuscript #21 and describes the mechanism of α2C-adrenoceptor translocation coupled to cyclic AMP-Rap1A intracellular signaling. The findings reported in this article were recommended for institutional press-release by the journal Editor-in-Chief.
23) Motawea HK, Blazek A, Zirwas M, Pleister A, Ahmed AA, McConnell BK, *#Chotani MA. Delocalization of endogenous A-kinase antagonizes cyclic AMP-Rap1-Rho-α2C-adrenoceptor signaling in human microvascular smooth muscle cells. J Cytol Mol Biol (Avens Publishing Group, USA and India, Open Access, ISSN: 2325-4653). 1 (1): 9; 2014 (PMID 24701590). IF: NA; Citations: 6 (new open-access journal launched 2013-2014)
This paper was curated by The Comparative Toxicogenomics Database (CTD), which is a USA National Institute of Environmental Health Sciences (NIEHS)-funded public database. The goal of CTD is to elucidate molecular mechanisms by which environmental chemicals affect disease. The CTD contains chemical-gene-disease interactions curated from the scientific literature. http://ctdbase.org/authordirect.go?acc=24701590
24) Pawlowski M, Saraswathi S, Motawea HKB, Chotani MA, Kloczkowski A. In-silico modeling of human α2C-adrenoceptor interaction with filamin-2. PLoS ONE (Public Library of Science, San Francisco, Open access, ISSN: 1932-6203) 9(8): e103099; Aug 11, 2014. doi:10.1371/journal.pone.0103099 (PMID 25110951). IF: 4.411; Citations: 8 (total views: 2,049; includes 350 downloads)
25) Khan I, Ali A, Akhter MA, Naeem N, Chotani MA, Tuba M, Salim A. Preconditioning of mesenchymal stem cells with 2, 4-dinitrophenol improves cardiac function in infarcted rats. Life Sciences (Molecular, Cellular and Functional Basis of Therapy) 162: 60-69; 2016 (PMID 27543341). IF: 2.685; Citations: 14
26) Khan I, Ali A, Akhter MA, Naeem N, Chotani MA, Iqbal H, Kabir N, Atiq M, Salim A. Epac-Rap1 activated mesenchymal stem cells improve cardiac function in rat model of myocardial infarction. Cardiovascular Therapeutics, 35 (2): e12248 (1-12); 2017 (PMID 28039940). IF: 2.478; Citations: 9
27) Motawea HKB, Chotani MA, Ali M, Ackerman W, Zho G, Ahmed AAE, Buhimschi CS, Buhimschi IA. Human placenta expresses α2-adrenergic receptors and may be implicated in pathogenesis of preeclampsia and fetal growth restriction. Am. J. Pathology (Cardiovascular, Pulmonary, and Renal Pathology) 188: 2774-2785; 2018 (PMID 30273604). IF: 4.07
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Invited Reviews
28) Jeyaraj SC, Unger NT, *#Chotani MA. Rap1 GTPases: an emerging role in the cardiovasculature. Life Sciences (Molecular, Cellular and Functional Basis of Therapy) (Netherlands, Elsevier Science, ISSN: 0024-3205), 88: 645-652; 2011 (PMID 21295042). IF: 2.685; Citations: 39
29) Chotani MA, Flavahan NA. Intracellular α2C-adrenoceptors: storage depot, stunted development or signaling domain? Biochimica et Biophysica Acta-Molecular Cell Research (Netherlands, Elsevier Science, ISSN: 0167-4889), 1813(8): 1495-1503; 2011 (PMID 21605601). IF: 5.128; Citations: 20
This collaborative effort with Johns Hopkins University included a timely discussion and review of G protein coupled receptors that are preferentially retained within intracellular ER and Golgi compartments, and focuses primarily on α2C-adrenoceptors as an example. This peer-reviewed article filled a gap in the field.
30) Flavahan, N.A., Flavahan, S., Mitra, S., Chotani, MA. The Vasculopathy of Raynaud’s Phenomenon and Scleroderma. In: Rheum. Dis. Clin. North Am. (Scleroderma; White B., Guest Editor) (WB Saunders Publisher, ISSN: 0889-857X), 29:275-291; 2003. (PMID 12841295). IF: 2.791; Citations: 98
Published Abstracts: 2013-Present (from 27 total)
1) #Chotani MA. Rap1A GTPase and the Vasculature: insights from studies on microvascular smooth muscle cells. Journal of Vascular Research. 53(1): 26 (2016). Presented at the “Mechanisms of Vasodilatation 12th International Symposium” at the Mayo Clinic Rochester, Minnesota (November 7-9, 2016).
2) #Chotani MA. Integrated Omics approaches to elucidate targets of cyclic AMP/Epac-Rap1A signaling in microvascular smooth muscle cells: identification of role in production and deposition of extracellular matrix fibrillary collagen. Circulation Research 119 (Suppl 1): A124 (2016). Peer-reviewed, and accepted for presentation at the American Heart Association Basic Cardiovascular Sciences Scientific Sessions 2016 July 18-21, Phoenix, Arizona.
3) Motawea H, Chotani M, Ahmed A, Zhao G, William A, Buhimschi CS, Buhimschi IA. “α2Adrenergic Receptors (α2ARs) in Pathogenesis of Preeclampsia: A Possible Role for (α2CARs) in Modulating Trophoblast Syncytialization”. Presented at the Society for Reproductive Investigation 63rd Annual Scientific Meeting, March 18, 2016, Montreal, Canada. Published in Reproductive Sciences, 23: 238A; 2016.
4) Motawea H, Zhao G, Summerfield T, Ackerman W, Gonzalez I, Chotani M, Buhimschi C, Ahmed A, Buhimschi I. “Evidence of presence and engagement of α2-adrenergic receptors (α2-ars) in the pathogenesis of preeclampsia”. Published in Am J Obstet Gynecol. 212 (1); S217, January, 2015.
5) Motawea HKB, Jeyaraj SC, Pawlowski M, Kloczkowski A, #Chotani MA. “Cyclic AMP-Rap1A signaling mediates cell surface translocation of microvascular smooth muscle α2C-adrenoceptors through the actin binding protein filamin-2”. Experimental Biology 2013, Boston, MA. Published in FASEB J. 27:729.15, April 9, 2013.
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6) Randive R, Jeyaraj SC, Chotani, MA, Stewart, Jr., JA. “Rap1a mediates extracellular matrix (ECM) remodeling through AGE/RAGE signaling in diabetes mellitus”. Experimental Biology 2013, Boston, MA. Published in FASEB J. 27:1188.4, April 9, 2013.
Intellectual Property
(US Patent & Trademark Office No. 6,444,681, WO Patent No. 2,000,076,502; Co-discoverer) “Methods and compositions for treating Raynaud's phenomenon and Scleroderma”
Editorial Assistance Contributor, “Paper Alerts: Pharmacology”, Current Opinion in Pharmacology (January 2001- September
2002) Assistant to the Guest Referee Editor, American Journal of Physiology (Fall 2001) Ad Hoc reviewer (scientific journals): J. Pharmacology & Experimental Therapeutics
Life Sciences Clinical Autonomic Research Journal of Cellular & Molecular Medicine
Journal of Cytology & Molecular Biology Editorial Board, Journal of Cytology & Molecular Biology; Journal of Advanced Pharmacy Research Invited Abstract Reviewer (Treatment of Arrhythmias: Pharmacologic category), Scientific Sessions 2016
and Resuscitation Science Symposium (ReSS), American Heart Association, Committee on Scientific Sessions Program, USA.
Invited talks
Chotani, M.A. “Gene regulation of human fibroblast growth factor-1”. Presented to The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute for Biological Studies, August 11, 1995, La Jolla, California.
Chotani, M.A. “Expression of 2-adrenoceptors in human vascular smooth muscle cells”. Presented to the Department of Biological and Biomedical Sciences, The Aga Khan University, College of Medicine, August 5, 2003, Karachi, Pakistan.
Chotani, M.A., Mitra, S., Eid, A.H., Flavahan, N.A. “Rap1 and filamin-2 regulate subtype-specific, cell-surface delivery of α2-adrenoceptors”. Featured Topic Session on Receptor Biology: intracellular signaling and second messengers, Experimental Biology 2007 Symposium, Washington DC, April 28-May 2, 2007.
Chotani MA. “Vascular α2C-adrenoceptors: from skin punch biopsy to the culture dish”. Invited lecture, 2nd International Workshop-cum-Training Course on Molecular Medicine and Drug Research, Jan 5-7, 2009, Karachi, Pakistan (unable to attend due to other commitments; sent regrets).
Chotani, M.A. “Regulated Cell surface delivery of α2C-adrenoceptors”: Invited session lecture 2009 Research Day, The Research Institute at Nationwide Children’s Hospital, May 15 th 2009 (CME approved presentation).
Integrated Biomedical Science Graduate Program (IBGP), and the Interdisciplinary Molecular, Cellular, & Developmental Biology (MCDB) Graduate Program, Ohio State University seminars (September 2, 2011,
Maqsood A. Chotani, PhD
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Graves Hall 1187, and September 13, 2011, 111 Parks Hall).
Chotani, M.A. Invited Works-in-Progress lecture, Department of Pathology, Ohio State University, October 6, 2011, 2-3 PM, Room 137 Hamilton Hall “Mechanisms of vascular α2C-adrenoceptor expression and mobilization”
Chotani MA. “Experimental and Computational Modeling Prediction Approaches To Elucidate Mechanism of Vascular α2C-Adrenoceptor Translocation”. Invited session lecture, Disease Related Proteins, Zing Conference on Mathematical and Computational Medicine, December 1- December 5, 2012, Mayan Riviera at the Occidental Grand Xcaret Resort, Playa del Carmen, Mexico.
Chotani MA. Invited lecture, 4th International Symposium-Cum-Training Course on Molecular Medicine and Drug Research (MMDR-4), Jan 7-10, 2013, Karachi, Pakistan (unable to attend due to other commitments; sent regrets).
Chotani MA. “Application of Experimental & Computational Modeling Prediction Approaches to Elucidate Vascular α2C-Adrenoceptor Function”. Invited session lecture, Collaborations: Role of Bioinformatics and Its Positive Impact on Translation Medicine, panelist (Nationwide Children’s Presents a Multifaceted Computational Approach to Pediatric Care), Bioinformatics in the Genome Era, Understanding the Evolving Role of Bioinformatics in Molecular Medicine, February 14, 2013, Molecular Med Tri-CON, Moscone North Convention Center, San Francisco, CA.
Chotani MA. Invited lecture, 5th International Symposium-Cum-Training Course on Molecular Medicine and Drug Research (MMDR-5), Jan 12-15, 2015, Karachi, Pakistan (unable to attend due to other commitments; sent regrets).
Chotani MA. “Discovering the Biological Role of Vascular α2C-Adrenoceptors: Novel Insights From Ex-Vivo, In-Vitro, and In-Silico Studies”. Invited Online Lecture, Virtual Education Project Pakistan, March 3, 2015, Video Conferencing Room, L.E.J. National Science Information Center, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Pakistan.
Chotani MA. “Targeting Intracellular α2C-adrenoceptors Using Experimental and In Silico Approaches”. Scientific Session on Molecular Methods, 4th Pak-France Bi-national Workshop on Drug Discovery & Molecular Medicine (October 13, 2015), International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Pakistan.
Chotani MA. “Rap1A GTPase and the Vasculature: insights from studies on microvascular smooth muscle cells”. Mechanisms of Vasodilatation 12th International Symposium at the Mayo Clinic Rochester, Minnesota USA (November 8, 2016).
Chotani MA. “The Small GTPase Rap1A: New Insights of Role in the Peripheral Circulation”. The 6th International Symposium-cum-Training Course on Molecular Medicine and Drug Research (MMDR-6), Karachi, Pakistan (November 6-9, 2017).
Chotani MA. “The α2C-adrenoceptors: uncovering the biological role in the peripheral circulation”. The 8th Scientific Meeting of the Asian Society for Vascular Biology, Shanghai, China (November 2-4, 2018).
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Teaching, Training/Mentoring, and Thesis Committees 2009 - Present (high school, undergraduate, graduate, and postdoctoral)
Past and Current Trainees
1. Paul N. El-Dahdah, BS (post-graduate). Accepted to medical school, Ohio University, Athens, Ohio USA. Paul was a Research Assistant in my lab.
2. Ryan C. Rauck (undergraduate). Accepted to medical school, Ohio State University, College of Medicine USA.
Ryan was a Pre-Medicine student at Johns Hopkins University, Baltimore, Maryland. Ryan worked in my lab during the summer of 2009 and received 3 course credits through Johns Hopkins University for Research (Biology 020.597). His primary project involved structure-function studies on the G protein coupled α2-adrenoceptors (α2A- and α2C-adrenoceptors). Specifically, domain swapping studies to elucidate the role of the carboxyl terminus in regulating receptor trafficking. He presented this work at the annual undergraduate Tri-Beta Biology Honor Society poster session at Johns Hopkins, and graduated with honors.
3. Robert Fidelibus (undergraduate, Aug 24, 2010 – June 7, 2012). Undergraduate studies, Chemical Engineering, Ohio State University, Columbus, Ohio USA
Robert was selected as an American Physiological Society (APS) Undergraduate Summer Research Fellow for 2011. Robert was one of 24 national winners. Robert’s project was developed further for an honors thesis for his graduation. He presented his work at the national Experimental Biology 2012 meeting in San Diego, CA. Robert accepted a position with The Dow Chemical Company (Engineers Driven for Growth & Excellence (Edge) Program) in New Orleans, LA USA.
4. Joel Eversole, BS (post-graduate, March 15, 2011 – July 31, 2012). Volunteer trainee. Joel received training in basic laboratory research techniques. He subsequently applied to schools with a graduate program in Physician Assistant Studies (PA Program).
5. Nicholas T. Unger, BA, MS (July 2009 - April 11, 20012). Research Associate, Center for Cardiovascular & Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio USA. Nick was initially a Research Assistant in my lab and was promoted. He completed his Master’s degree and transitioned to a full-time job with a local company.
6. Selvi C. Jeyaraj, PhD (Postdoctoral, March 2009 - March 23, 2012). Selvi was a trainee in my lab at the Center for Cardiovascular & Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio USA. Selvi was supported by a T32 training grant. She chose a career in teaching and was Adjunct Faculty, Department of Biological Sciences, Capital University, Columbus, Ohio, prior to her relocation to Kentucky.
7. Hanaa K.B. Motawea, MS (February 29, 2012 - 2017). Hanaa was a visiting graduate student and part of a joint PhD supervision program with Helwan University, Cairo, Egypt. Hanaa completed her PhD requirements in 2017.
8. Muzzammil Ahmed (March 16, 2012 – August 21, 2012). Muzzammil was a sophomore at Dublin Coffman High School, Dublin, Ohio USA, and was an Intern in my laboratory.
9. Ramsha Khurshid, Sabah Farhat, Nimra Naz Siddiqui, Hira Jawed, Syed Hira Hassan, Hadiqa Nasir, Mashal Anis, Departments of Physiology, Biotechnology, and Biochemistry MSc Program, University of Karachi (June-July 2015, 2016, 2017, 2018), Summer Interns, Dr. Panjwani Center for Molecular Medicine
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& Drug Research, International Center for Chemical & Biological Sciences, University of Karachi, Pakistan (Summer Internship Program for Youth).
10. Hina Hazrat, Shafaq Javaid, Sadaf Zubair, Sadaf Shaikh, Dur-e-Shahwar, Durreyshahwar, (2015 – present), M.Phil. students, Dr. Panjwani Center for Molecular Medicine & Drug Research, International Center for Chemical & Biological Sciences, University of Karachi, Pakistan.
11. Saima Mohsin, (2018-present), PhD student, Dr. Panjwani Center for Molecular Medicine & Drug Research, International Center for Chemical & Biological Sciences, University of Karachi, Pakistan.
12. Bushra Taj Muhammad Khan, PhD student (Co-supervisor), Dr. Panjwani Center for Molecular Medicine & Drug Research, International Center for Chemical & Biological Sciences, University of Karachi, Pakistan. Bushra completed her degree requirements in 2017.
Rotation graduate students 13. Brian Jan (Molecular, Cellular and Developmental Biology Graduate Program, Ohio State University USA,
June 28-July30, 2010). 14. Shengwei Chang (Molecular, Cellular and Developmental Biology Graduate Program, Ohio State
University USA, July1-August 5, 2010). 15. James Boslett (Molecular, Cellular and Developmental Biology Graduate Program, Ohio State University
USA, January 3, 2012- March 9, 2012). 16. Alisa Blazek (Molecular, Cellular and Developmental Biology Graduate Program, Ohio State University
USA, March 26, 2012- June 7, 2012).
Maqsood A. Chotani, PhD
12
Current and Past External Funding as Principal Investigator (PI)
PI (name): MAQSOOD A. CHOTANI
Sr. # Title of Project
Initiation date
Completion date
Amount(s) requested/ awarded
Funding source(s)
1. “Deciphering the Role of the Small GTPase Rap1A in Microvascular Remodeling”
(HEC/R&D/NRPU/2017/7440) National Research Program for Universities
(Approved for funding) Role: PI
Pending funds release
36 months
PKR:
3.07 million (requested)
Higher Education Commission (Islamabad, Pakistan)
2. “Microvascular α2C-adrenoceptors: Targeting Protein-Protein Interactions for Treatment of
Peripheral Vascular Disease” (5742/Sindh/NRPU/R&D/HEC/2016)
National Research Program for Universities (Active) Role: PI
January, 2018
36 months (On-going)
PKR:
16.927585
million (awarded)
Higher Education
Commission (Islamabad, Pakistan)
3. “Mechanism of Vascular α2C-adrenoceptor Mobilization” (No. R21HL088087)
(Completed) Role: PI
March 04, 2008
February 28, 2012
US $: Direct costs:
275,000 Indirect costs:
137,500
National Heart, Lung, and Blood
Institute, NIH (Bethesda, United
States) 4. “Targeting Expression and Mobilization of
Vascular α2C-adrenoceptors” (No. 0765204B) (Completed) Role: PI
July 01, 2007 June 30, 2010 US $: Direct costs:
110,000 Indirect costs:
11,000
American Heart Association
(Texas; Ohio Valley Affiliate, United States)
5. “Role of α2-adrenoceptors in Vascular Smooth Muscle Cell Biology: Toward Development of an
In-Vitro Model for Scleroderma” (No. 9920625V)
(Completed) Role: PI
July 01, 1999 June 30, 2001 US $:
60,000
American Heart Association
(Texas; Ohio Valley Affiliate, United States)
Maqsood A. Chotani, PhD
13
Other Support (MAQSOOD A. CHOTANI)
Sr. # Type of Support
Initiation date
Completion date
Amount(s) awarded
Funding source(s)
1. Graduate Research (MPhil Scholarships, total 6) Primary Role: Faculty Supervisor
August 2015
On-going
PKR:
16,000/month
(each)
1.152 million/year
Dr. Panjwani Center for Molecular Medicine and Drug Research,
International Center for Chemical & Biological Sciences, University of
Karachi, Karachi, Pakistan
2. International Scholarship Primary Role: PhD Advisor (Joint Supervision Program with Helwan University, Cairo, Egypt) (Completed)
2012
2013
US $:
40,000/year
Government of Egypt
3. Institutional training grant Primary Role: Sponsor Lab (Completed)
January 2010
December 2011
US $:
97,790
T32 (NIH, USA)
4. Travel award (Postdoctoral Trainee) Primary Role: Mentor (Completed)
2010 2010 US $:
1,200
American Society for Biochemistry &
Molecular Biology, USA
5. Summer Undergraduate Research Fellowship (10 weeks) Primary Role: Research Advisor/Host Lab (Completed)
2011 2011 US $:
5,600
American Physiological Society,
USA
Maqsood A. Chotani, PhD
14
Graduate/Undergraduate Committees (MAQSOOD A. CHOTANI)
Sr. # Trainee Name
Role on Committee Document Type; Title
1. Elizabeth C. McNally (Graduate Program in Education: Teaching &
Learning, Ohio State University)
Graduate Faculty Representative final oral
examination for doctoral degree (August 6, 2010)
(PhD) “Examining literacy development:
Differential participation in narratives and literacy practices in one linguistically
diverse kindergarten classroom” 2. Brian E. Jan
(Molecular, Cellular, and Developmental Biology; Ohio
State University)
Graduate Committee member for non-thesis written and oral
Master’s examination
(MS)
“Collagen and the Heart”
3. Nicholas T. Unger (Biophysics Graduate Program;
Ohio State University)
Master’s Thesis Committee (Winter 2012)
(MS) “Blockade Of The Transient Receptor
Potential Vanilloid (TRP) by Ruthenium Red Does Not Suppress Hypothalamic
Neuronal Thermosensitivity” 4. Zoltan I. Buzas
(Political Science; Ohio State University)
Graduate Faculty Representative Final Oral
Examination for doctoral degree (June 29, 2012)
(PhD) “Race and International Politics: How
Racial Prejudice Can Shape Discord and Cooperation among Great Powers
5. Robert Fidelibus (Chemical Engineering; Ohio
State University)
Undergraduate Honors Research Thesis Committee
(Spring 2012)
(BS) “Peptide expression and purification to
elucidate the mechanism of vascular α2C-adrenoceptor translocation”
6. Hanaa KB Motawea (visiting graduate student, Department of Pharmacology & Toxicology,
Helwan University, Cairo, Egypt)
PhD Advisor (May 2017)
(PhD) “Pharmacological and Molecular
Approaches to Elucidate Mechanism of Vascular α2C-Adrenergic Receptor
Translocation” 7. Bushra Taj Muhammad Khan
(Dr. Panjwani Center for Molecular Medicine & Drug
Research, International Center for Chemical & Biological
Sciences (PCMD, ICCBS), University of Karachi)
PhD Co-Advisor (Nov 2017)
(PhD) “Identification of Tyrosine
Phosphorylated Proteins Causing Hyperphosphorylation and Apoptosis Resistance in Human Lymphoma Cell
Lines”
8. Hina Hazrat (PCMD, ICCBS, University of
Karachi)
MPhil Advisor (Aug 2015 - Present)
(MPhil; studies ongoing) “Behavioral Phenotyping and
Neurotransmitter Analysis of Rap1 Subtype A (Rap1A) GTPase Knockout
Mice”
Maqsood A. Chotani, PhD
15
Graduate/Undergraduate Committees (MAQSOOD A. CHOTANI) - continued
Sr. # Trainee Name
Role on Committee Document Type; Title
9. Shafaq Javaid (PCMD, ICCBS, University of
Karachi)
MPhil Advisor (Aug 2015 - Present)
(MPhil; studies ongoing) “Elucidating the role of Rap1 subtype A
(Rap1A) GTPase in the Cardiovascular System”
10. Sadaf Shaikh (PCMD, ICCBS, University of
Karachi)
MPhil Advisor (Aug 2016 - Present)
(MPhil; studies ongoing) “Proteomic Analysis of Rap1A GTPase-
deficient C57BL/6J Mouse Heart” 11. Sadaf Zubair
(PCMD, ICCBS, University of Karachi)
MPhil Advisor (Aug 2016 - Present)
(MPhil; studies ongoing) “Studies of the Small GTPase Rap1 in
Insulin Regulation In Vivo” 12. Dur-e-Shahwar
(PCMD, ICCBS, University of Karachi)
MPhil Advisor (Aug 2017 - Present)
(MPhil; studies ongoing) “Targeting microvascular α2C-
adrenoceptor protein-protein interaction using in-silico and experimental
approaches” 13. Saima Mohsin
(PCMD, ICCBS, University of Karachi)
PhD Advisor (Aug 2018 – Present)
(PhD; studies initiated)
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