Isb presentation

ISB - Ion Source & Biotechnologies

"We semplify mass spectrometry world"

Simone Cristoni, Ph.D.Founder & CEO

Cell: +39-340-963-9745Cell: +39-340-963-9745

Email: [email protected]

ISB-Headquarters & Laboratories: Insubrias BioBiopark, Via Roberto Lepetit 34

Gerenzano (VA) – Italy. Tel. 02/96474438; Fax 02/700410252.

Company Overview

Founded on September 5th, 2006

ISB Core Business

Development and Engineering of new Ionization Sources for

Mass Spectrometry

3 Italian government grants have financed ISB start-up

and its initial growth

Core Business

SERVICES

ISB offers an extensive

array of services, ranging

from development of

customized analytical

APPLICATIONS (kits)

ISB has developed a

series of standardized

analytical methods that

can be directly used in

IONIZATION SOURCES

ISB has patented two

innovative ionization

sources for mass

spectrometry allowing forcustomized analytical

methods, to the analysis

of specific proteins and

biomolecules.

can be directly used in

our laboratory or

transferred to the

customer.

spectrometry allowing for

a considerable reduction

of analysis times and

costs.

Multidisciplinary Scientific Team

• Lorenzo Zingaro - Chemist;

• Vincenza Valveri - Biologist;

• Cristina Canton - Pharmacologist;

• Vincenza Flora Dibari - Bioinformatics;

ISB experienced and multidisciplinary technical team iscomplemented by a talented group of PhD andundergraduate students recruited through collaborations withthe University of Milan.

Strategic Location

Insubrias BioPark

is one of the eight technological parks and bio-incubators of the Italianis one of the eight technological parks and bio-incubators of the Italian

biotechnology sector. It is located in Gerenzano, Varese. The BioParkcovers an area of 52,000sm; 15,000sm of which include office space

and laboratories, equipped with the most advanced technologies in thebio-medical sector.

The BioPark is located in a beautiful green area in a geographical and economical

strategic position aiming at creating an effective network of partners in the territory. It was

created by the cooperation of Italian and Swiss entrepreneurship found in the historical

area called Insubriac Region, shared by Lombardia, Piemonte and Cantone Ticino.

Regio Insubria and Insubrias BioPark

A "Euroregion”,

complying with thecomplying with the

Madrid Agreement by

the Council of Europe

which since 1995

promotes cross-

boundary cooperation in

the Italian-Swiss region

of the three pre-Alpine

lakes regions including

Lake Como, LakeLake Como, Lake

Lugano and Lake

Maggiore.

Insubrias BioPark

Mission

• Make mass spectrometry easy: Produce highly

innovative and ready to use kits for clinical analysis

based on proprietary Ionization Sources for mass

spectrometry.

• Assay Development Program: develop a custom

tailored research and development program using a

strong in-house technological expertise.strong in-house technological expertise.

• Services Program: provide state-of-the-art services to

our customers based on innovative proprietary

technologies.

Why ionization source

Ionization source is a key component of mass

spectrometry influencing the following key factors:

• Sensitivity: it is a function of ionization efficiency and

chemical noise produced by ion charged solvent

clusters.

• Quantitative Accuracy: Matrix effect dependent.

• Throughput: a source that exhibits high sensitivity and

low matrix effect can in principle increase the

throughput.

Company structure

ISB ionization sources portfolio

• Surface Activated Chemical Ionization (SACI; Cristoni • Surface Activated Chemical Ionization (SACI; Cristoni

S. et al. Mass Spectrom Rev. 2007 Sep-Oct;26(5):645-

56);

• Universal Soft Ionization Source (USIS; Cristoni S.

International Patent n. WO/2007/131682);International Patent n. WO/2007/131682);

Mass analyzers

• HCT ultra Ion Trap (Bruker Daltonics);

• ORBITRAP high resolution mass spectrometry

(Thermoelecton);

Surface Activated Chemical Ionization(SACI)

Cristoni et al. Mass Spectrom Rev. 2007 Sep-Oct;26(5):645-

56.

SACIBenefits

• Increased sensitivity operating in full scan (chemical

noise reduction).

• Lower matrix effect.

• Operates in ion exchange chromatographic conditions.• Operates in ion exchange chromatographic conditions.

SACIChemical noise reduction

Figure 1 – Analysis of Cocaine and Morphine, HCT & APCI

Retention time (minutes)

Sig

nal I

nte

nsity

Figure 2 – Analysis of Cocaine and Morphine, HCT & SACI

Cocaine

Morphine

Retention time (minutes)

Sig

nal I

nte

nsity

SACILimitations

• Compound dependent (It is particularly suitable for

peptide and protein analysis).

• Not very effective for Biomarker Discovery.

Universal Soft Ionization Source(USIS)

Reference patent

USISBenefits

• Increased sensitivity operating in full scan (chemical

noise reduction).noise reduction).

• Lower matrix effect.

• Operates in ion exchange chromatographic conditions.

• Can ionize all kind of polar and not polar compounds.• Can ionize all kind of polar and not polar compounds.

• Ideal for full scan profiling (e.g. Bacterial

Characterization through metabolite profiling).

USISApplications

Polar Low polar

USISLimitations

• The detectable m/z ratio depends on the mass

analyzer.

Future development

• USIS for breath analysis.

• Deposit of other provisional patents to improuves

ionization source performances.

ISBContacts

Contact us:

Dr. Simone Cristoni

Tel: +39-02-96474438

Cel: +39-340-9639745

e-mail: [email protected]

Web page: http://www.isbiolab.comWeb page: http://www.isbiolab.com

Technology

Isb presentation