CLINICAL MICROSCOPY/UBF

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Definition

Clinical Microscopy (MTE07)

• The course deals with the macroscopic, chemical and microscopic study of the different non-blood body fluids and their by products

Clinical Microscopy

• urine, feces, bronchial and bronchoalveolarlavage, sputum, gastric and duodenal contents, pancreatic exocrine secretions, cerebrospinal fluid, synovial fluid, serous fluids (transudates and exudates), sweat, seminal fluid and amniotic fluid.

• Collection, handling, processing, reference ranges and clinical significance

PRE-REQUISITES:

MICROBIOLGY

PHYSIOLOGY

COURSE CREDIT:

2 units lec; 1 unit lab

REQUIREMENTS:1. Color code: YELLOW (LAB)

ORANGE (LEC)2. Orange highlighter and

blue ballpen3. Activity notebook4. Lecture notes &

compilation of hand-outs5. Portfolio – deadline (final

exam)6. Textbook7. Quota – processing of

specimen and case report, 5 per person (20% of FE)

8. Lab: preliminary reports, post-exam conference, protocols, data sheets

9. PPE; specimen; Group materials

10. Conference – prelims (case ana); SF (quiz show)

QA and Safety

• Basic terms

• Quality Assurance

• Laboratory Safety– Biological hazards

– Sharp hazards

– Chemical hazards

– Radioactive hazards

– Electrical hazards

– Fire/explosive hazards

– Physical hazards

Microscopy

• Types of Microscopy

• Brightfield Microscope

• Care and Use of the Microscope

Renal Function

• Renal Anatomy

• Renal Blood flow

• Renal Physiology– Functions

– Processes• Glomerular Filtration

• Tubular Reabsorption

• Tubular Secretion

Urinalysis

• Introduction (History, Urine formation, composition, volume, specimen collection, handling)

• Physical examination of urine

• Chemical examination of urine

• Microscopic examination of urine

• Other methods

Renal and Renal-Associated Dses

• Glomerular

• Tubular

• Interstitial

• Vascular

• Renal Failure

• Renal lithiasis

• Metabolic disorders

Other Exams for Renal Dses

• Basic terms

• Urinary sediment Quantification

• Stone Analysis

• Assessment of GFR

• Concentration tests

• Tubular Secretory fxn tests

OBF’s

• Pregnancy Test and HCG Assay

• CSF

• Seminal fluid

• Synovial fluid

• Serous fluid

• Amniotic fluid

• Sweat

• Bronchial washings, bronchoalveolar lavage & sputum

• Gastric fluid, Duodenal content and pancreatic exocrine secretion

• Fecal analysis

References

Brunzel, Nancy A. Fundamentals of Urine and Body Fluid

Analysis. W.B. Saunders, Philadelphia. 1994

Henry, John Bernard et al. Clinical Diagnosis by Laboratory

Methods. 20th Edition, W.B. Saunders, Philadelphia.

2001

Estridge, Barbara et al. Basic Medical Laboratory

Techniques. 4th Edition. Delmar, USA. 2000

Leihmann, Craig A (ed). Saunder's Manual of Clinical

Laboratory Science. W.B. Saunders, Philadelphia. 1998

References

McBride, Landy J. Textbook of Urinalysis and Body Fluids: A

Clinical Approach. Lippincott, Philadelphia. 1998

Sacher, Ronald A & McPherson, Richard A. Widmann's

Clinical Interpretation of Laboratory Tests. 11th Edition.

F.A. Davis Company, Thailand. 2000

Strasinger, Susan K. & Di Lorenzo, Marjorie S. Urinalysis

and Body Fluids. 5th Edition. F.A. Davis Company,

Thailand. 2008