A member of CGIAR consortium www.iita.org

Biotechnology

and

Genetic Improvement

R4D Week 2015

A member of CGIAR consortium www.iita.org

Strategic Objectives:

1. Enhancing the management,

characterization, and use of

plant genetic resources

2. Enhancing the efficiency and

effectiveness of plant breeding

3. Efficient diagnosis and

monitoring of biological systems

Biotechnology Strategy

Cryo Bank

A member of CGIAR consortium www.iita.org

Genetic Improvement Strategy

Strategic Objectives

1.Increased yields of staple crops

2.Increased resilience of staple crops to biotic and abiotic

stresses

3.Increased nutrient density and suitability of staple crops to

new utilization pathways

4.Raised efficiency of breeding staple crops

5.Enhanced germplasm of staple crops and increased

potential of selected underutilized crops

A member of CGIAR consortium www.iita.org

Management and utilization of genetic resources

Application of tools and methods for genotyping and

phenotyping

Transgenic approaches

Data capture and management systems

Strengthening partnerships and capacity building

Deployment of knowledge and products

Enabling resources and technologies

A member of CGIAR consortium www.iita.org

Cassavabase:

A global open

access resource

All data available with Open Access with agreement to observe the

Toronto Protocol regarding use of unpublished data

Databases

A member of CGIAR consortium www.iita.org

Regional Cassava Breeders

Meeting West Africa and DRC

A member of CGIAR consortium www.iita.org

1. Integrated Genotyping Service and Support (IGSS) (BecA-

ILRI Hub)

2. Shared Industrial-Scale High-Throughput & Low cost

Genotyping Facility (ICRISAT )

3. Integrated Breeding Platform (including the Breeding

Management System)

4. Improving Breeding Program Effectiveness with the Breeding

Program assessment Tool (University of Queensland )

5. Genetic Gain Platform?

Other Initiatives

A member of CGIAR consortium www.iita.org

Crop Country No. of Varieties Released with Partners

2013 2014 2015

Cassava Liberia - 3 -

Nigeria - 3 -

DRC 6 - -

Sierra Leone - 2 -

Tanzania - - 4

Uganda - - 2

Cowpea Nigeria - - 2

Sierra Leone - - 2

Burkina Faso 2 - -

Swaziland - - 6

Tanzania 2 - 2

Maize Benin - - 6

Ghana - - 5

Mali 2 6 -

Nigeria 5 4 4

Soybean Nigeria - 2 -

Sierra Leone - - 4

Zambia - - 2

A member of CGIAR consortium www.iita.org

Post-release

Seed companies [maize]

Community-based seed

production [cowpea, soybean,

maize]

Outgrower schemes/

development agencies

Bilateral projects [cassava

(5CP, Hplus, seed syst.

Nigeria), yam, cowpea, maize]

A member of CGIAR consortium www.iita.org

Sensitive & Robust Virus Diagnostic Tools

Developed Type Purpose Stage of development

Multiplex RT-PCR Simultaneous detection

of 3 RNA viruses

Completed

Direct Binding PCR Episomal yam

badnavirus detection

Validation

Antibodies for YMV,

YMMV and Badnavirus

ELISA, IC-PCR and

LFD based diagnostics

Validation

RT-LAMP and RPA On-site tool kit Validation & standardization

for on-site use

Single tube multiplex PCR RT-LAMP

A member of CGIAR consortium www.iita.org

1. Sampling yam young vines 2. In vitro introduction

Meristem excision, culture and plantlet re-growth (2 to 6 months)

Bulk propagation of meristem cultures by nodal cuttings

(2 - 3 months)

Tuber planting in field or screenhouse. Virus indexing and selection for in vitro induction

(1 to 3 months)

3. In vitro nodal cultures

Virus indexing

Virus indexing by multiplex RT-PCR on in vitro samples

(1 week)

Re-indexing for reconfirmation (2 to 6 months)

4. Acclimatization for re-indexing

Bulk propagation of virus-free plants by in vitro nodal cuttings or vine propagation

(2 to 4 months)

5. Selection of virus-free plants & bulking

Establishing clean seed stocks

A member of CGIAR consortium www.iita.org

Temporary Immersion Bioreactor

Aeroponic System

Circumvents dormancy period

Links to vine propagation

Perennial growth habit

Pre-basic and basic seed production

Rapid Propagation

A member of CGIAR consortium www.iita.org

Crop breeding programs

Phenotyping

Stresses; GIS; Morphology; Nutritional & end-use quality

Genotyping, Data Management & Mechanization

Cross-cutting topics

Private sector breeding; Delivery of products; Communication

Satellite Meetings

QTL, MAS, BMS

Epigenetics and Gene Expression

Breeding Values

Workshop on Genetic Improvement Sept.

A member of CGIAR consortium www.iita.org

Genebank data and information management system Marimagne Tchamba

Recent advances in phenotyping advanced drought

tolerant and striga resistant maize genotypes for

tolerance to combined drought and heat stress

Silvestro Meseka

Regeneration and genetic transformation of yam Rajesh Manoharan

Towards molecular breeding in banana: challenges and

opportunities working with perennial polyploid crops

Allan Brown

Enhancing proVitamin A in cassava breeding using new

tools

Elizabeth Parkes

Presentations

A member of CGIAR consortium www.iita.org

Thank you